Vemuri Rudra Subramaniyam – Unlocking the Secrets of Biotechnology: My Journey from University to Groundbreaking Research

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Vemuri Rudra Subramaniyam
New Delhi, India

Profile Summary
Biotechnologist with a strong foundation in molecular biology and microbiology. Hands-on experience in culture and cultivation of E. coli culture, tobacco, and Drosophila systems for Genotypic and phenotypic research. Adept in collaborating in research teams and maintaining laboratory. Proficient in experimental design, data analysis, and interpretation for impactful research outcomes.

Key Skills
– Gateway cloning
– Fluorescence microscopy
– RT-PCR
– Bacterial and yeast culture
– SDS PAGE
– Specimen processing
– Effective team collaboration
– Time management
– Presentation

Education
Master of Science in Biotechnology and Enterprise | University of Manchester
September 2023 – September 2024
– Relevant coursework: Commercialisation of life sciences, research skills (lab-based research project), Advanced Biotechnology: expression systems for biopharmaceutical and industrial proteins, synthetic biology, disease modeling and genome editing.
– Passed without backlogs
– Projects:
– ELISA test kit development using Chromition lumispheres.
– Functional Analysis of Proteins for Plant Cellulose Synthase Complex trafficking.

Bachelor of Science in Biotechnology | Manav Rachna International Institute of Research and Studies, Faridabad, Haryana
June 2019 – May 2023
– Relevant coursework: Bioinformatics, cell biology, microbiology, molecular biology, immunology, Fermentation technology, animal biotechnology, plant biotechnology, food technology, molecular diagnostics, cell and tissue culture, stem cell technology.
– Achieved 7.5 CGPA (75%), graduated with Distinction
– Leadership Roles: Head of Environ Club, Class Representative
– Projects:
– Biodegradable packaging from wheat straw.
– Genetic and phenotypical analysis of Drosophila melanogaster.

Projects and Research Experience
Research Intern | University of Manchester | Jan 2024 to Sep 2024
– Executed Gateway cloning for over seven cDNA samples.
– Bacterial and Yeast cell culture.
– Conducted transfection procedures on Arabidopsis and Tobacco plants.
– Protein purification using biotinylated protein pulldown assay.
– Cultivated Arabidopsis strains to support university research.
– Documented experimental results and collaborated with senior researchers on project design and data interpretation.

Research Intern | Indian Agricultural Research Institute, Pusa | Jan 2023 to July 2023
– Engaged in research titled Understanding DWnt4 functions during embryo patterning and ovary development in Drosophila melanogaster.
– Crossing Drosophila for phenotypical analysis and performed ovary dissections for GFP and DAPI stained fluorescence analysis across five phenotypes.
– Organized and maintained Drosophila culture tubes and presented research insights to senior management.

Certifications
– Cell Culture Technologies (NPTEL)
– Cancer Biology (Coursera)
– Protein Structure Prediction and Molecular Docking Workshop (NPTEL)
– RT-PCR Workshop (Bionest Bioincubator, RCB, Faridabad)
– New Age Diagnostics Workshop (Bionest Bioincubator, RCB, Faridabad)

Skills
Gateway cloning, Fluorescence microscopy, RT-PCR, Bacterial and yeast culture, SDS PAGE, Specimen processing, Effective team collaboration, Time management, Presentation
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Cover Letter:

**Subject:** Application for Microbiologist Position – Clinical & Diagnostic Laboratory
Dear Hiring Manager,
I am writing to express my strong interest in the Microbiologist position, as outlined in your job description. With a Master’s degree in Biotechnology and practical experience in both molecular and microbial diagnostics, I am confident in my ability to contribute meaningfully to your clinical microbiology and infection control efforts.
During my academic and internship tenures, I gained hands-on experience in culturing and analyzing various microbial specimens, performing biochemical and molecular tests such as PCR and ELISA, and adhering to strict biosafety protocols. At the Indian Agricultural Research Institute, I worked on _Drosophila melanogaster_ models, conducting fluorescence microscopy and genetic analysis—developing precision, documentation skills, and a strong foundation in experimental techniques.
My training has instilled a deep understanding of pathogen identification, antimicrobial resistance monitoring, and infection control standards. I am also proficient in data interpretation, maintaining microbial culture repositories, and collaborating across multidisciplinary teams. I am especially drawn to roles that integrate diagnostics with infection prevention and antimicrobial stewardship—areas where I believe microbiologists play a critical, proactive role.
I am detail-oriented, organized, and passionate about contributing to high-quality laboratory science in clinical settings. I would be honored to support your mission in enhancing diagnostics, reducing healthcare-associated infections (HAIs), and improving patient outcomes through laboratory excellence.
Please find my CV attached for your review. I would welcome the opportunity to discuss how my skills and background align with your team’s goals.
Thank you for your time and consideration.
Warm regards,
**V Rudra**

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