Tiyasha Koley – Unlocking the Secrets of Biotechnology: The Rising Star You Need to Know!

Tiyasha Koley

Biotechnologist

Electronic City Phase 2, Bangalore, 560100

Summary

Entrepreneurial individual with hands-on experience in gene cloning, PCR, and molecular biology, seeking a challenging position to leverage expertise in biotechnology and bioinformatics.

Experience

Online Tutoring, Freelance Subject Matter Expert (2023 – ongoing)

Research Experience

Telscie Genetics Pvt Ltd, Durgapur, West Bengal (January 2023 – March 2023)

• Spearheaded a research project by BIRAC
• Executed microbial culturing, screening, competent cell preparation, gene cloning, transformation
• Proficiently conducted SDS PAGE, western blotting, PCR, RT-PCR, DNA/RNA/Protein Extraction and isolation

Internship

“Biomedical Sciences” (University of Kalyani, July 2022 – August 2022)

• Acquired profound theoretical and practical knowledge about biochemical analysis and immunological analysis

Education

• [hidden] in Biotechnology, University of Burdwan
• [hidden] in Biotechnology, Panskura Banamali College

Project

• Initiated and executed a study on the “IN VITRO ANTHELMINTIC ACTIVITY OF AKANDA (Calotropis gigantea) WHOLE PLANT METHANOLIC EXTRACT IN INDIAN ADULT EARTHWORM”

Dissertation

• Investigated the “MECHANISM OF INFLAMMATION AND ITS ASSOCIATED PATHOGENESIS”

Core Competencies

• Molecular biology
• Biochemistry
• Computational Biology
• Next-Generation Sequencing (NGS)
• Understanding of Genetic Disorders
• Critical Thinking and Problem-Solving
• Collaboration and Communication Skills
• Microsoft Office

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Cover Letter:

Respected recruiter,
Following your advertisement on Biotechnika, I would like to submit my application for Junior Genome analyst. I have done [hidden] Biotechnology.
Previously, I worked on a BIRAC project for ‘The development of novel antibacterial therapeutics & recombinant protein production’ at Telscie Genetics Pvt. ltd. in Durgapur, West Bengal. I attained as a trainee in Biomedical sciences in Dept. of Biophysics and Biochemistry, at Kalyani University. Moreover, I completed an Animal cell culture-certified course from the department of Zoology at Kalyani University. Also, I have done an internship as a trainee in Molecular biology and HLA lab (COVID LAB) at Medica Superspeciality Hospital, Kolkata.
Some of the key requirements that I have gained expertise with include:-
1. **DNA/RNA/ Protein isolation and extraction, Electrophoresis, qPCR, RT PCR, Colony PCR, Recombinant protein extraction and purification, Immunological techniques, SDS-PAGE, Gibson Cloning, Restriction Digestion and Western Blotting.**
2. **Protein separation, Protein Isolation, Western Blotting, Affinity chromatography.**
3. **Biochemical tested, ELISA, RFLP, Microscope, Handling Spectrophotometer, auto pipettes, pH meter, Incubator, and Spectrophotometry.**
4. **Bioinformatics Software Knowledge of software such as BLAST, Bioconductor, alignment tools.**
5. **Next-Generation Sequencing (NGS) Familiarity with NGS platforms like Illumina, PacBio, Knowledge of genetic disorders and ACMG guidelines**
6. **Critical Thinking and Problem-Solving Ability to navigate through complex genomic data and tackle challenges during analysis.**
7. **Animal cell culture.**
8. **Python(Basic), MS Office.**
Please find my resume attached for your review. I look forward to the opportunity to discuss how my background and skills align with the needs in more detail. I believe that given a chance I will prove my above mentioned credentials in an interview.
Sincerely,
Tiyasha Koley