SUSHANTIKA RAJPUT – Discover the Future of Biotechnology: A Rising Star in Metagenomics and Microbial Research

SUSHANTIKA RAJPUT

PROFILE

I am a motivated and enthusiastic biotechnology graduate. I am passionate about research. Specialization in metagenomics and next-generation sequencing (NGS), with a focus on analysing microbial communities. Experienced in NGS library preparation, advanced microbiological techniques, and proficient in experimental design and data analysis. I have acquired hands-on expertise in microbiology, molecular biology, and bioinformatics to study the diverse and functional aspects of microbial communities. Currently, I am involved in a project that focuses on metagenomics, where I aim to unravel the changes in microbial communities across different environments. I’m eager to use my knowledge and skills to contribute to the progress of microbiome research and biotechnological advancements.

SKILL SETS

• Uterine swab collection from Buffalo
• DNA isolation, Gel Electrophoresis
• Conventional PCR and Digital PCR
• Library Preparation for 16S sequencing
• Microbiome Analysis
• Bioinformatics – Python, R, DADA 2
• Microbiological Techniques
• Competent Cell Preparation and Transformation
• Protein purification
• Microscopy
• Spectrophotometry, Basics of LC-MS

EDUCATION

• Gujarat Biotechnology University, Gujarat (2022-24)
[hidden] in Biotechnology, [hidden] CGPA
• Specialization in Animal Biotechnology- Animal Breeding, Genome Selection and others
• Additional Subjects- Entrepreneurship and Innovation, Product Discovery and Development, Data Science, Science Communication

• Sri Ramswaroop Memorial University, Lucknow (2019-22)
[hidden] in Biotechnology, [hidden] CGPA

RESEARCH PROJECT

‘-Omics’ studies of Endometritis in Buffaloes
My research focuses on investigating endometritis in buffaloes by comparing the microbial compositions of healthy individuals with those affected by the disease. Furthermore, I aim to assess the impact of different treatments, such as Normal Saline and antibiotics, on these microbial communities. By collecting uterine swab samples, performing DNA extraction, and employing sequencing techniques, particularly from microbial communities, I aim to precisely identify and characterize various microbial species within each sample. This will be achieved through the utilization of software tools like DADA2 to ensure accurate analysis and interpretation of the sequencing results.

TRAINING / WORKSHOPS

• MRD Life Sciences Pvt Ltd
  • 2 months training (Nov 2020 – Jan 2021)
  – Microbiological Techniques (Antibiotic Sensitivity Test, Minimum Inhibitory Concentration)
  – DNA isolation
• Attended workshop on Entrepreneurship by iRISE at IIT Gandhinagar
• Participated in GenVision organized by IIT Bombay

AWARDS & ACHIEVEMENTS

• GAT-B Qualified (2022)
• Silver Medal in Bachelors (2022)

REFERENCES

• Dr Varun Shah (Assistant Professor) Gujarat Biotechnology University
  [hidden]
  [hidden]
• Dr Vishal Suthar (Associate Professor) Gujarat Biotechnology University
  [hidden]
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KEYWORDS

Biotechnology, Research, Metagenomics, NGS, DNA Isolation, PCR, Bioinformatics, Microbiology, Data Analysis, Protein Purification, Spectrophotometry

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Cover Letter:

I have completed my master’s degree in biotechnology from Gujarat Biotechnology University. My dissertation project was on the topic “Omics analysis of Endometritis in buffaloes” where I have analyzed the bacterial diversity during endometritis condition, healthy and after giving them treatment. I have performed 16s rRNA sequencing of all the three kinds of samples collected from the uterus of buffaloes. And then I have analyzed the sequenced data using DADA2 software.