SAKSHI NAUTIYAL

MSc Microbiology

Nauti, Uttarakhand, 246487

Summary

Accomplished Microbiology Researcher with over 3 years of experience integrating biotechnological techniques and high-quality microbial research. Demonstrated ability to optimize laboratory operations and deliver comprehensive reports aligned with strategic scientific objectives, with a passion for advancing innovations.

Experience

JRF/PRS-I – NIPER-Mohali, Punjab

Worked as Junior Research Fellow/ Project Research Scientist-I in a project entitled “Development of a generic method for aptamer-based detection of protein oligomers” under the guidance of Prof. Ipsita Roy, Head of Department, Department of Biotechnology, NIPER, SAS Nagar, Mohali, Punjab, from March 2022 to November 2024.

Dissertation – ICMR JALMA, Agra

Completed Master’s thesis entitled “Expression Analysis of Chemokine Receptor (CXCR2) and Vascular Endothelial Growth Factor (VEGF) during Mycobacterium tuberculosis in Lungs of Guinea Pig (Cavia porcellus) Using Real Time PCR” under the guidance of Dr. D. S. Chauhan (Scientist-E), Head of Department, in Microbiology and Molecular Biology division, ICMR JALMA, Agra from Feb, 2021 to Sep, 2021.

Responsibilities

Master of Science in Microbiology – Hemvati Nandan Bahuguna Garhwal University, Srinagar, Garhwal Uttarakhand (2019-2021) – 7.86/10

Bachelor of Science – Hemvati Nandan Bahuguna Garhwal University, Srinagar, Garhwal Uttarakhand (2016-2019) – 8.05/10

Expertise

• Proficient in BSL-II and BSL-III laboratory practices
• Molecular cloning techniques such as cloning and expression in E. coli, clone generation, and assessment in shake flask
• Aptamer design and selection using SELEX technology
• Protein purification using FPLC and other methods
• Proficient in molecular biological techniques like PCR, RT-PCR, and more
• Worked in animal cell-culture and cell-based assays
• Hands-on trained on Guinea pig handling and related techniques

Publications

• Kaur, J., Nautiyal, S., & Roy, I. (2025) Harnessing RNA Aptamers to Disrupt Oncogenic HSP90α: A Targeted Approach. Journal of Medicinal Chemistry. (Submitted)
• Sarkar, S., Sharma, M., Karmakar, A., Jahan, K., Saproo, S., Biswas, S., Nautiyal, S., Roy, I., Bharatam, P.V., & Naidu, S. (2025). Selective Inhibition of RNA Polymerase I Transcription by NSH76 Demonstrates Potent Anti-cancer Activity. European Journal of Medicinal Chemistry. (Submitted)

References

Prof. Ipsita Roy, Dept. of Biotechnology, NIPER-SAS Nagar

Email Id: [Email Hidden]

Dr. Vineet Kumar Maurya, Assistant Professor, Dept. of Botany and Microbiology, HNBGU

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Skills

BSL-II, BSL-III, Molecular Cloning, Aptamer Design, Protein Purification, PCR, RT-PCR, Cell Culture, Cell-Based Assays, Guinea Pig Handling

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Cover Letter:

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