Sakshi Devram Shenkar – Unlocking the Secrets of Biotechnology: A Rising Star in Cancer Research

Sakshi Devram Shenkar

Location: Pune, India

Email: [hidden]

Mobile: [hidden]

Profile

Highly motivated and detail-oriented postgraduate with a Master of Science in Biotechnology seeking a position to gain knowledge and start my career. Possesses a strong foundation in scientific principles and laboratory techniques, with a dedication to continuous learning and skill development.

Education

• MSc Biotechnology 2022-2024
  [hidden] Y. Patil Arts, Commerce and Science, Pimpri Pune
  CGPA: 8/10
• BSc Biotechnology June 2019-2022
  D Y Patil International University, Akurdi Pune
  CGPA: [hidden]
• HSC Science (Physics, Chemistry, Biology, Mathematics) Feb 2019
  Abhishek Arts, Commerce and Science college
  Percentage: 67%
• SSC March 2017
  Abhishek Vidyalayam, Pune
  Percentage: 83%

Technical Skills

|Fluorescence in Situ Hybridization |DNA/RNA Isolation |Antibiotic Sensitivity testing| Microbial culture and isolation |Confocal Microscopy |Fluorescence Microscopy ELISA |PCR| Western Blotting | SDS-PAGE |

Experience

1. Breast Cancer Research: Laboratory Techniques, and Diagnostics
   Dr. [hidden] Patil Medical College, Hospital & Research Centre, Pune Jan 2024 – June 2024
   • Extensive experience working with breast cancerous tissue (DCIS, HER2 positive breast cancer tissue)
   • Skilled in Fluorescence in Situ Hybridization (FISH) technique, including deparaffinizing slides and HER 2-probe hybridization
   • Performed IHC staining on breast cancer tissues (HER2, DCIS)
   • Experienced in preparing Standard Operating Procedures (SOPs) for laboratory protocols and techniques
   • Proficient in analyzing results, interpreting data, and making detailed reports of the results
2. Bioinformatics – Dr. [hidden] Patil Arts, Commerce and Science College, Pune
   • Performing molecular docking simulations with AutoDock to evaluate ligand-binding affinities and interactions with protein targets
   • Proficient in using BLAST for sequence comparison and identifying homologous sequences to understand functional and evolutionary relationships
   • Capable of predicting tertiary protein structures using SWISS-MODEL for modeling protein sequences and inferring functional properties
3. Mammalian Cell Culture technology – Dr. [hidden] Patil Arts, Commerce and Science College, Pune
   • Mastered advanced mammalian cell culture techniques, including media preparation, subculturing
   • Expertly established primary cell cultures
   • Performed Giemsa Staining, Karyotyping and MTT assay

Projects

1. Standardization of HER2(Human Epidermal Growth Factor Receptor 2) by Fluorescent in situ hybridization (FISH) Jan 2024 – July 2024
   Department of Cytogenetics, Dr. D Patil Medical College, Hospital and Research Center

   Aim: The study aims to differentiate HER2-positive and HER2-negative gene in breast cancer tissue using fluorescence in situ hybridization when IHC results are equivocal.

2. Isolation and Characterization of Bacteria from Drinking Water in Pune, Maharashtra, and their Antibiotic Profiling March 2022
   Department of Biotechnology, D Y Patil International University

   Aim: The study involves the isolation and characterization of bacteria from water samples, along with evaluating the in-vitro susceptibility of bacterial isolates to various antibiotics.

3. Study of alpha-amylase activity of wheatgrass from Lokwan wheat variety of Pune (local market) and their purification Feb 2021
   Department of Biotechnology, D Y Patil International University

   Aim: The objective of this study was to quantify α-amylase activity, protein purification, protein content estimation in Lokwan wheat germinating seeds and wheatgrass.

Seminars and Conferences

• Virtually attended The Lancet Breast Cancer Commission and key findings Conference March 2024
• National seminar on “Innovative Practices in Life Sciences” Organized by Department of Life Sciences and Internal Quality Assurance Cell
• Participated virtually in the I-OSICON 2024 conference, and attended session on future of Immunotherapy in India

Languages

English, Hindi, Marathi

Hobbies

Learning new things, making network, Books Reading, Cooking

Declaration

I, Sakshi Devram Shenkar, hereby declare that the information contained herein is true and correct to the best of my knowledge and belief.

Date: Sakshi D. Shenkar

Place: Pune

Technical Skills (Keywords)

Fluorescence in Situ Hybridization, DNA/RNA Isolation, Antibiotic Sensitivity testing, Microbial culture and isolation, Confocal Microscopy, Fluorescence Microscopy, ELISA, PCR, Western Blotting, SDS-PAGE

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Cover Letter:

Subject: Application for Genome Analyst Position at MedGenome Labs

Dear Hiring Manager,

I am excited to apply for the Genome Analyst position at MedGenome Labs. With a Master’s degree in Biotechnology and a strong background in molecular biology, I am eager to contribute to your esteemed organization’s pioneering work in clinical genomics.

During my academic and research journey, I have gained hands-on experience on fluorescence in situ hybridization (FISH). As a Research Intern at Dr. DY Patil Medical College, I worked on gene detection, fluorescence imaging, and HER2 analysis, refining my expertise in genomic research. Additionally, my work in microbial isolation, antibiotic profiling, and molecular diagnostics has strengthened my analytical and laboratory skills.

MedGenome’s focus on cutting-edge sequencing platforms and clinical genomics aligns with my aspiration to work at the forefront of personalized medicine and genetic research. I am particularly interested in variant interpretation, QC analysis, and molecular assay development, and I am eager to apply my skills to support your mission in advancing genomic insights.

I would welcome the opportunity to discuss how my expertise aligns with your team’s goals. Thank you for your time and consideration. I look forward to the possibility of contributing to MedGenome Labs.

Best regards,
Sakshi Devram Shenkar