Raj Kishore – Unveiling the Secrets of Antimicrobial Resistance: Meet the Microbiologist Revolutionizing Peptide Biology

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Raj Kishore, PhD

Professional Summary

A dedicated and accomplished microbiologist with a PhD in Microbiology from CSIRCentral Drug Research Institute (CSIR-CDRI), Lucknow, specializing in peptide biology, antimicrobial resistance, and ESKAPE pathogens. Proven expertise in high-throughput in silico screening, peptide synthesis, microbiological assays, and preclinical evaluation of novel peptide mimetics. Skilled in animal handling, cell culture, molecular biology techniques, and advanced bioinformatics tools. Published multiple research articles and contributed to patented innovations in antimicrobial peptide development.

Areas of Expertise

  • Microbiology: ESKAPE pathogens, antimicrobial resistance, biofilm studies, virology, mycology.
  • Peptide Biology: Design, synthesis, and evaluation of antimicrobial and antiviral peptides.
  • Molecular Biology: DNA/RNA isolation, PCR, RT-PCR, Western blotting, gel electrophoresis.
  • Virology: RNA isolation and diagnostics of Covid 19 virus through RT-PCR.
  • Mycology: Screening of compounds/nanoparticles against candida and aspergillus species.
  • Hybridoma technology: Monoclonal antibody production.
  • Animal Models: Thigh infection, lung infection, wound infection, and burn wound models.
  • Bioinformatics: In silico screening, peptide design, Primer design.
  • Instrumentation: HPLC, lyophilizer, spectrophotometer, nanodrop, RT-PCR, BSL-2 laminar airflow.

Education

PhD in Microbiology
CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, India

Master of Science ([hidden]) in Microbiology
Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India

Bachelor of Science ([hidden]) in Chemistry and Microbiology
Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India

Professional Experience

ICMR-Senior Research Fellow (Peptide Biology)
CSIR-Central Drug Research Institute, Lucknow, India

Key Projects

  1. Major Project: Exploring the Sensitivity of ESKAPE Pathogens Towards Novel Peptide Mimetics: An answer to antimicrobial resistance

Skills

  • Peptide Synthesis: Designing and Solid-phase peptide synthesis (SPPS).
  • Animal Handling: Swiss, Balb/C, and C57BL/6 mice models.

Publications and Patents

  1. Publications: List of published research articles
  2. Manuscript Under Preparation: Ongoing research work
  3. Patents: Granted and pending patents
  4. NCBI Submissions: Submissions related to research

Scholastic Achievements

Achievements related to academic and professional qualifications.

Conferences and Workshops

  • Details of conferences and workshops attended and presentations given.

References

  1. Dr. Mukesh Pasupuleti
  2. Dr. Rabi Sankar Bhatta
  3. Dr. Raja Gopal Rayavarapu

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Cover Letter:

Sure! Here is the redacted version of the text you provided:

Dear Sir,

I am writing to express my keen interest in the position junior research scientist at the Molpath pvt ltd. I hold a [hidden] in Microbiology from CSIR–Central Drug Research Institute (CSIR-CDRI), Lucknow, India. With over seven years of experience in drug development and screening within the R&D division at CSIR-CDRI, I am eager to contribute my expertise to your research initiatives.

My doctoral research focused on “Exploring the Sensitivity of ESKAPE Pathogens Towards Novel Peptide Mimetics: An Answer to Antimicrobial Resistance,” emphasizing the development of peptide-based therapeutics against drug-resistant pathogens. Throughout my [hidden] tenure, I have gained extensive experience in drug screening and optimization using various microbiological and cell culture assays.

In addition to my research experience, I possess expertise in a wide range of technical skills, including:

Microbiological and cell culture assays
ELISA, Western blotting, and monoclonal antibody development
Antifungal and antiviral screening (Covid 19 diagonastics)
PCR, RT-PCR, and HPLC
Development of infectious disease models, such as lung infection, thigh infection, and burn wound models
My multidisciplinary background in infectious disease modeling, immunology, and antimicrobial therapeutics enables me to contribute effectively to your team. I have a strong publication record in peer-reviewed journals and experience in patent drafting, reflecting my commitment to scientific innovation and translational research.

I would welcome the opportunity to discuss how my skills align with your research objectives. Please find my CV attached for your review. Thank you for your time and consideration. I look forward to the possibility of contributing to your team.

Best regards,
Raj Kishore
[hidden] Microbiology

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