Raj Kishore – The Microbiologist Who’s Changing the Game in Antimicrobial Research!

Raj Kishore, PhD

Professional Summary

A dedicated and accomplished microbiologist with a PhD in Microbiology specializing in peptide biology, antimicrobial resistance, and ESKAPE pathogens. Experienced in high throughput in silico screening, peptide synthesis, microbiological assays, and preclinical evaluation of novel peptide mimetics. Skilled in animal handling, cell culture, molecular biology techniques, and bioinformatics tools. Published multiple research articles and contributed to patented innovations in antimicrobial peptide development.

Areas of Expertise

• Microbiology: ESKAPE pathogens, antimicrobial resistance, biofilm studies, virology, mycology.
• Peptide Biology: Design, synthesis, and evaluation of antimicrobial and antiviral peptides.
• Molecular Biology: DNA/RNA isolation, PCR, RT-PCR, Western blotting, gel electrophoresis.
• Virology: RNA isolation and diagnostics of Covid 19 virus through RT-PCR.
• Mycology: Screening of compounds/nanoparticles against candida and aspergillus species.
• Hybridoma technology: Monoclonal antibody production.
• Animal Models: Thigh infection, lung infection, wound infection, and burn wound models.
• Bioinformatics: In silico screening, peptide design, Primer design.
• Instrumentation: HPLC, Lyophilizer, spectrophotometer, nanodrop, RT-PCR, BSL-2 laminar airflow.

Education

PhD in Microbiology
CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, India

Master of Science ([hidden]) in Biotechnology
Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India

Bachelor of Science ([hidden]) in Chemistry and Microbiology
Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India

Professional Experience

ICMR-Senior Research Fellow (Peptide Biology and Antimicrobial resistance)
CSIR-Central Drug Research Institute, Lucknow, India

Project Associate (Antiviral Research Diagnostics)
CSIR-Central Drug Research Institute, Lucknow, India

Project Junior Research Fellow (MOES Drugs From Sea)
CSIR-Central Drug Research Institute, Lucknow, India

Project Assistant (MOES Drugs From Sea)
CSIR-Central Drug Research Institute, Lucknow, India

DBT BCIL Industrial Trainee
Helix Biogenesis Pvt. Ltd., Noida, India

Key Projects

• Major Project: Exploring the Sensitivity of ESKAPE Pathogens Towards Novel Peptide Mimetics
• Minor Projects: In-vitro Screening of Small Molecules, Antibiofilm Activity of Antibiotics Tagged to Amino Acids, Antimicrobial Screening of Nanoparticles

Skills

• Peptide Synthesis: Designing and Solid-phase peptide synthesis (SPPS)
• Animal Handling: Swiss, Balb/C, and C57BL/6 mice models
• Cell Culture: Maintenance of A549, VERO, THP-1, and BV2 cell lines
• Bioassays: MIC, MTT, LDH, hemolysis, ELISA, stability assay, antibiofilm assays
• Formulations: Liposome preparation and calcien based leakage assay
• Software: Sigma Plot, GraphPad Prism, EndNote X9, Adobe Photoshop, MS Office, Biorender and AI based software
• Writing skills: Manuscript, patent drafting and grant writing
• Research training: Mentoring students in microbiological techniques and experimental design

Publications and Patents

Publications and patents information can be found in the provided links.

Scholastic Achievements

Qualified ICAR-NET, GATE, and ICMR Direct-SRF

Conferences and Workshops

• 3rd International Conference on Antimicrobial Resistance (New Delhi, 2024)
• Two-Day International Conference on Microbial Odyssey (Gujarat, 2023)
• Workshop on Wet Chemical Synthesis of Nanoparticles (Lucknow, 2021)

References

• Dr. Mukesh Pasupuleti – Senior Principal Scientist, CSIR-CDRI, Lucknow
• Dr. Rabi Sankar Bhatta – Senior Principal Scientist, CSIR-CDRI, Lucknow
• Dr. Raja Gopal Rayavarapu – Principal Scientist, CSIR-IITR, Lucknow

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