R. SRIMATHI

MICROBIOLOGIST

PROFESSIONAL SUMMARY

Experienced microbiologist with solid expertise in research laboratory settings in Central government institutions. Strong experience performing biochemical and molecular biology experiments, as well as implementing Good Laboratory Practices, analysis, and data collection.

EXPERIENCE

SGS India Pvt Ltd. – Chennai | April 2019 – August 2019

• Performed routine microbiological testing on drug products, raw materials, and stability samples, ensuring compliance with USP, EP, and IP standards.
• Conducted environmental monitoring, including air sampling, surface sampling, and microbial isolation identification.
• Executed microbiological assays such as total microbial count and selective culturing for objectionable organisms.
• Identified microbial isolates using Vitek-2 Compact, ensuring accurate classification of contaminants.

TRAINEE MICROBIOLOGIST – CSIR-NEERI, Chennai Zonal Center | February 2020 – March 2022

• Extracted metagenomic DNA from deep-sea sediment, analyzed Nanopore sequencing data, and screened Type III PKS genes.
• Conducted microbial water quality analysis, investigated antibiotic-resistant bacteria, and participated in COVID-19 surveillance.
• Compiled biodiversity and environmental data for a Puducherry biodiversity compendium and documented research findings.

PROJECT ASSOCIATE-I – ICAR-Central Institute of Brackishwater Aquaculture, Chennai | June 2022 – February 2023

• Investigated antimicrobial properties of herbal extracts, evaluated antiparasitic efficacy, and extracted RNA for molecular analyses.

YOUNG PROFESSIONAL-I – Caldor Health Tech, Atal Incubation Centre, CSIR-CCMB, Hyderabad | September 2023 – November 2023

• Conducted viability assays on goat cartilage samples and monitored cellular responses after electromechanical reshaping.
• Performed data analysis on viability assay results and assisted in optimizing experimental protocols.

RESEARCH ASSISTANT – National Institute of Animal Biotechnology, Hyderabad | November 2023 – November 2024

• Investigated drug resistance mechanisms, cloned and expressed parasite genes, and performed gene expression analysis.
• Generated antibodies for downstream applications and conducted CRISPR-Cas9-mediated knockout experiments.

EDUCATION

• B. SC. in Microbiology from Ethiraj College for Women (May 2017)
• M. SC. in Applied Microbiology from SRM Arts and Science College (May 2019)

REFERENCES

• Dr. Rajesh T – CSIR-NEERI, Chennai | Senior Scientist | [hidden]
• Dr. Poornima – ICAR-CIBA, Chennai | Principal Scientist | [hidden]

PROJECTS

• Assessment of the bio-absorption potential of lead-resistant bacteria from E-waste dumpyards in Chennai.
• Preparation of Compendium on Environmental Statistics in the [hidden] of Puducherry.
• Restoration and Rejuvenation of lakes in the Vrushabavathi river valley.
• Monitoring Multi-drug resistance microbial communities in natural water bodies during lockdown.
• Functional characterization of Polyketide synthase genes from Deep sea sediments.
• Comprehensive analysis of microbial communities conferring antibiotic resistance.
• Evaluation of herbal formulations against Vibrio and Pseudomonas species.

CERTIFICATIONS

• Genome Informatics certification from Decode Life Sciences and Ensembl

AWARDS

• 1st Place in ‘Microcosm’ exhibition for ‘Elephantiasis’ model presentation.
• 1st Place in Model presentation on ‘Global warming’.

SKILLS

RT-PCR, Fluorescence microscopy, Western Blot, Cell Culture, Molecular cloning, Recombinant protein expression, CRISPR Gene editing

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Cover Letter:

Dear Hiring manager
I hope you are doing well. I recently came across the Research Scientist position at Molpath pvt Ltd and found the opportunity highly aligned with my expertise in mammalian cell culture and [hidden] nearly four years of research experience, I have developed a strong background in cell culture, molecular biology, and drug resistance research across academia and industry. In my previous roles:
– At BRIC-NIAB, I worked on Theileria annulata, focusing on molecular cloning, recombinant protein purification, and drug resistance mechanisms using RT-PCR and epigenetic analysis. I also developed mammalian cell culture-based assays to evaluate drug efficacy on parasitic cell lines.
– At Caldor Health Technologies, I worked on electromechanical reshaping (EMR) technology for nasal cartilage, conducting viability assays, fluorescence microscopy, and optimizing experimental conditions across various electrical parameters. I analyzed tissue integrity under different electrode configurations, gaining insights into biomedical device development and bioengineering applications.
– At ICAR-CIBA, I conducted antimicrobial and antiparasitic studies on herbal extracts, assessing their efficacy against Enterocytozoon hepatopenaei (EHP), a shrimp pathogen. Our research aimed to develop safe, plant-based therapeutics to combat drug resistance in aquaculture.
– At CSIR-NEERI, I identified Polyketide Synthase, a potential anticancer compound, from deep-sea sediment metagenome, successfully cloning, expressing, and processing the protein for downstream applications. I also investigated antibiotic-resistant bacteria in environmental water samples during the COVID-19 lockdown, strengthening my expertise in molecular cloning, protein expression, and microbial resistance.
– At SGS India Pvt Ltd, I developed skills in pharmaceutical product testing, quality control, microbial identification, and environmental monitoring.
Given my diverse experience in cell culture and I am eager to contribute to your cutting edge research in cell culture optimization, microfluidics and translational medicine. Thank you for your consideration.
Sincerely yours,
Srimathi. R