Personnel Profile
Pyareelal Sahoo
Anlabereni-759026, Dhenkanal, Odisha, India
Career Objective:
Recent graduate in applied microbiology with a good focus on microbial genomics and bioinformatics. Committed, knowledgeable, and experienced in project and team management. Seeking a position that provides lab experience to develop practical skills in various fields of life sciences.
Academic Qualifications:
| S. No. | Examination Passed | Board/University | Year | Marks (%) | CGPA (Out of 10 scale) |
|---|---|---|---|---|---|
| 1 | [hidden] Applied Microbiology | Vellore Institute of Technology, Vellore, Tamil Nadu | 2022 | [hidden] CGPA | |
| 2 | [hidden] Microbiology (Hons) | College of Basic Science and Humanities, OUAT, Bhubaneswar, Odisha | 2020 | [hidden] CGPA | |
| 3 | 12th | CBSE | Ghanashyam Hemalata Vidyamandir, Puri, Odisha | 2017 | 71% |
| 4 | 10th | ICSE | Montfort School, Dhenkanal | 2015 | 65% |
Research Experience:
I have worked in the Molecular Medical Bacteriology Lab at National Institute of Science Education and Research (NISER), Bhubaneswar, under the guidance of Dr. Harapriya Mohapatra. The work was completed for my MSc Dissertation over a period of eight months in 2022.
Thesis entitled “INVESTIGATING ANTIBIOTIC INDUCED PERSISTENCE IN CLINICAL ISOLATES OF Klebsiella spp.“
Technical Skills:
- Microbiology Techniques:
- Culturing and aseptic techniques
- Bacteria enumeration
- Identification of pathogen
- Confocal Microscope
- Antimicrobial Testing
- Molecular Techniques:
- DNA and RNA extraction, estimation and quantification
- Agarose gel electrophoresis, SDS PAGE, Western Blotting
- Polymerase chain reaction (PCR)
- Gene expression and Quantitative Real Time PCR (qPCR)
Computational Skills:
- Molecular-docking tools:
- Auto-dock
- Patch-dock
- Pymol
- MS Office:
- Word
- Excel
- PowerPoint
Project:
During my MSc, I completed a project work for the Science Engineering and Technology (SET) Conference titled “Analysis of Beneficial Binding Affinity of the Active Compounds of Andrographis paniculata with the Measles Virus Protein; An In-silico Virtual Docking Approach” under the guidance of Dr. [hidden] Sabina from School of Bioscience and Technology (SBST), VIT, Vellore.
Publication:
N., Sangeetha & Seenivasan, Vijayadharsini & J., Vaishnavi & Mishra, Subhashree & Purushottam, Sunkari & Sahoo, Pyareelal & Evan, Prince. (2021). Analysis of Beneficial Binding Affinity of the Active Compounds of Andrographis paniculata with the Measles Virus Protein: An In-Silico Virtual Docking Approach. Research Journal of Biotechnology. 17. 102-110. [hidden]
Award:
Raman Research Award for publication in Scopus Indexed Journal in February 2022 by Vellore Institute of Technology (VIT), Vellore.
Declaration
I hereby state that all the information noted above is accurate to the best of my belief and I take full responsibility for the correctness of the information.
Pyareelal Sahoo
Date: 04/02/2024
Skills Keywords:
Microbiology Techniques, Molecular Techniques, DNA extraction, PCR, Gel electrophoresis, Microscopy, Data analysis, Project management, Teamwork, Communication skills
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Cover Letter:
I am Pyareelal Sahoo from Dhenkanal district of Odisha. I am a MSc graduate in the field of applied microbiology from Vellore Institute of Technology (2022). With a strong background in bioinformatics and genomic data interpretation, I am eager to contribute my expertise to your team.
While working on Klebsiella pneumoniae for my master thesis in NISER, I have screened around several strains of Klebsiella pneumoniae to find out persister cells that cause opportunistic infections such as urinary tract infection, pneumonia, pyogenic liver abscesses, and surgical site infection. The goal was to study and look for persister cells in Klebsiella pneumoniae clinical isolates and look into the genes implicated in non-genetic tolerance through different antimicrobial susceptibility testing, biphasic killing assays, heritability assays, extraction of total cellular RNA, and gene expression profiling by quantitative real-time PCR (qPCR) in persister cells generated in response to levofloxacin therapy.
Apart from this field, I have some basic knowledge in different bioinformatics and molecular docking tools, which I gained while working on a SET project during my post-graduation in VIT. Measles is a highly infectious viral disease that affects the respiratory tract. Even though the vaccine was developed, it still concerns a group of populations in the world. A medicinal plant, Andrographis paniculata, which is locally called Green Chiretta, is an annual herbaceous plant that has numerous medicinal properties. The thirteen compounds from Andrographis paniculata were virtually docked with the major receptor proteins of measles using the Patch-Dock program for molecular docking. The results obtained were analyzed, and the docked structures are visualized using PyMOL software.
I believe my foundational knowledge of these core concepts and research experiences will contribute towards making me a good fit for the job role at your company. I will do my best to meet the high standards of your company on this count.
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