Prafulla Srivastava – From Lab Novice to Machine Learning Pioneer: The Journey of a Rising Biotechnologist

Prafulla Srivastava

Profile

Dynamic and passionate biotechnologist with a strong foundation in both wet and dry lab practices. Proficient in
handling advanced laboratory instruments and techniques, including SDS-PAGE, chromatography, plant tissue
culture, and in silico gene characterization. Actively participated in esteemed national and international
conferences, showcasing research aptitude and networking with global experts. Completed comprehensive training
programs in biotechnology, enhancing technical and analytical skills. A well-rounded individual with leadership
experience in cultural events, demonstrating exceptional teamwork and organizational abilities. Driven to excel in
innovative biotechnological research and contribute to groundbreaking advancements.

Professional Experience

2024/06 – 2025/05
Project on Machine learning approach to find novel anti-diabetic drug from natural sources
Biotecnika Info Labs Pvt Ltd
Role: Research Associate/Project Lead

Responsibilities:

– Designed and implemented a data-driven workflow integrating machine learning models
and bioinformatics tools for drug discovery.
– Curated and pre-processed datasets of natural compounds, including their
physicochemical properties, ADME profiles, and bioactivity data.
– Extracted molecular descriptors such as molecular weight, LogP, and Ali Log S using
cheminformatics tools like RDKit and SwissADME.
– Developed and validated predictive machine learning models (Random Forest, Decision
Tree, Linear Regression) to evaluate compound efficacy and drug-likeness.
– Conducted statistical analysis and visualization of data using Python

2022/06 – 2022/07
Lucknow, India
Trainee
Experiome Biotech Private Limited

Completed training in biotechnology, acquiring expertise in wet lab techniques such as
SDS-PAGE, chromatography, plant tissue culture, microbiology lab culture, and agarose
gel electrophoresis. Proficient in dry lab practices like in silico gene family
characterization. Skilled in laboratory protocols, data analysis, and molecular biology
research methodologies.

2024/08 – 2025/03
Trainee
Biogrademy

Learning basics of python and bio python libraries

2022/02
Lucknow, India
Trainee
CytoGene Research & Development LLP

Training of basic bioinstrumentation tools like protein quantification assays, Agarose gel
electrophoresis and DNA extraction from various sources

Student Coordinator
Apni Pathshala, SRMU

Part of CSR initiative of the University at Apni Pathshala runs for taking care of primary
studies of the students of surrounding villages

2025/01 – 2025/06
Project Dissertation
National Centre for Cell Science

– Investigated the role of secretory PD-L1 (secPD-L1) in modulating T-cell exhaustion
and apoptosis using Jurkat E6.1 cell line models
– Designed and optimized Western blot, pull-down assays, and co-immunoprecipitation
to analyze PD-L1/PD-1 and FBXO family protein interactions
– Hands-on experience in protein concentration (cut-off 3kDa), SDS-PAGE, PVDF
transfer, antibody probing, and chemiluminescence detection
– Performed ELISPOT/activation assays and used Jurkat + co-culture systems to validate
secretory PD-L1’s functional role

Education

2023/07 – present
Hisar, India
M.Sc. Biotechnology
Guru Jambheshwar University of Science and Technology

Highly motivated MSc Biotechnology graduate with hands-on experience in advanced
laboratory techniques, including column chromatography, centrifugation, SDS-PAGE,
PCR, UV spectroscopy, and agarose gel electrophoresis. Proficient in handling laboratory
equipment with precision and adhering to strict safety protocols. Actively participated in
multiple national and international conferences, showcasing research work and engaging
with global scientific communities. Demonstrates strong analytical skills, a commitment
to innovation, and a passion for contributing to cutting-edge biotechnological
advancements.

2020/08 – 2023/06
Barabanki, India
B.Sc. (Hons) Biotechnology
Shri Ramswaroop Memorial University

Skills

– PCR
– Chromatography
– Gel electrophoresis
– HPLC
– Protein Purification
– Cell Proliferation Assays (MTT, CCK-8)
– Machine learning
– Cell cultures
– Python
– Molecular Cloning
– Immunoprecipitation (IP)
– Scientific Writing

Languages

– English
– Hindi

Certificates

– Volunteer at AMI International Conference 2024
– Best Actor Award at Freshers Harmony 2022 at SRMU
– Coordinator at Shaswat Fest at SRMU
– Certified in HPLC, GC, and PCR handling
– Part of NSS Republic Day parade
– Sports Coordinator at Annual Sports Fest Ullas 2023 at SRMU
– Student Coordinator International Conference on Advances in Cancer Biology, Prevention & Therapy, SRMU 2022

Cover Letter:

Prafulla Srivastava
Ayodhya, Uttar Pradesh 224001

Date: May 26,2025

To,
The HR Department
Panacea Medical Technologies Pvt. Ltd.
Malur, Near Devanahalli, Karnataka

Subject: Application for the Position of Clinical Research Associate

Dear Hiring Manager,

I am writing to express my keen interest in the Clinical Research Associate (CRA) position at Panacea Medical Technologies Pvt. Ltd., as advertised on Biotecnika. With a robust academic foundation in Biotechnology and hands-on experience in both wet and dry lab environments, I am confident in my ability to contribute effectively to your clinical research team.

Currently, I am pursuing my M.Sc. in Biotechnology from Guru Jambheshwar University of Science and Technology, and I am engaged in my project dissertation at the National Centre for Cell Science (NCCS). My research involves functional validation of the secretory PD-L1 (secPD-L1) protein in immune modulation, using techniques such as Western blotting, ELISPOT, co-culture assays, and flow cytometry. Additionally, I am working on a machine learning-based anti-diabetic drug discovery project with Biotecnika Info Labs Pvt. Ltd., where I developed predictive models using molecular descriptors and cheminformatics tools.

My experience has allowed me to develop a deep understanding of clinical research methodologies, data integrity, GCP guidelines, and regulatory compliance. I am well-versed in data collection and statistical analysis using Python, and possess strong skills in documentation, communication, and interdisciplinary collaboration.

Panacea Medical Technologies’ commitment to advancing medical technologies through clinical excellence aligns perfectly with my career aspirations. I am excited about the opportunity to contribute to your mission and gain valuable experience in clinical trial execution, monitoring, and reporting.

I have attached my resume for your consideration. I would be grateful for the opportunity to discuss my application further and demonstrate how I can contribute to your clinical research operations.

Thank you for considering my application. I look forward to the opportunity to contribute to your esteemed organization.

Warm regards,
Prafulla Srivastava