POORNIMA KALAL – Unlocking the Secrets of Life: How a Genetics Expert is Changing the Game

POORNIMA KALAL

EDUCATION

Master of Science in Applied Genetics – 2012

Bachelor of Science in Chemistry, Zoology, Genetics – 2010

Karnataka University, Dharwad

RELEVANT SKILLS

Molecular Biology Techniques

• Polymerase chain Reaction
• Blood DNA and RNA Isolation
• Nucleic acid Quantification
• Gel Electrophoresis

Bioinformatics Tools and Techniques

• Knowledge of Genomics and Genetics
• Sequence alignment tools FASTQC
• Molecular Docking tools: Auto Dock, PyRx
• PubChem, PubMed
• NGS data analysis
• Python Coding
• Sanger Sequence Analysis
• Primer Designing
• Molecular Databases: NCBI, EMBL, DDBJ, PDB, UCSC

Cell Culture Techniques

• Handling of Cancer cell lines (H157, U1752, A549)
• Preparing anti-cancer drug resistant cell lines
• Cell counting, Drug treatment to find IC50 of cancer cells

PROFESSIONAL EXPERIENCE

Research Associate

2019 – 2020

Center for Human Genetics, Bangalore, Karnataka

• Handling and maintaining of mammalian (Cancer) cell lines.
• Learnt basic cell culture techniques with media preparation.
• Prepared anti-cancer drug resistant cell lines.
• Performed work according to project schedules.
• Helped with preparation of project related reports

Research Associate

2014 – 2015

Center for Human Genetics, Bangalore, Karnataka

• Worked on Human Genetic disorders like Rett’s syndrome and Epidermolysis Bullosa.
• Did Blood DNA Isolation, Quantification, setting up PCR and sent samples for Sequencing.
• Done the Sequence analysis (Sanger) of diseased samples over more than 100 patients using Sequencher tool.
• Performed work according to project schedules and established quality standards

CERTIFICATIONS

• 6-Months Offline-Internship with hands on project in Bioinformatics Tools and Techniques from Biotecnika, Bangalore.
• Certified Summer Research follow from Indian Institute of Sciences, Bangalore

SKILLS

Molecular Biology, Bioinformatics, Cell Culture Techniques, Genomics, Genetics, Sequence Analysis, Python, Molecular Databases, Drug Discovery, Project Management

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Cover Letter:

Hiring Manager
MedGenome

**Subject: Application for Genome Analyst Position**
Dear Hiring Manager,
I am excited to apply for the Genome Analyst position at MedGenome. With a strong background in bioinformatics, molecular biology, NGS, human genetics, and genome sequencing, I am eager to contribute my expertise to your team.
I hold an MSc in Applied Genetics from Karnataka University, Dharwad, and have extensive experience in genome analysis, next-generation sequencing (NGS), and bioinformatics tools. My work at the Center for Human Genetics involved analyzing genetic disorders, including Rett syndrome and Epidermolysis Bullosa, where I performed DNA sequencing, variant analysis, and data interpretation for over 100 patients. Additionally, my current research integrates computational biology techniques to identify pathogenic variants and molecular mechanisms underlying genetic disorders.
With my ongoing project at Biotecnika following a bioinformatics internship, I have gained experience in primer designing, molecular docking, and drug discovery applications and published a paper on “Identifying pathogenic variants and molecular mechanisms underlying genetic [hidden]#8221;
I am eager to apply my analytical skills and scientific knowledge to support MedGenome’s research initiatives.
I welcome the opportunity to discuss how my expertise can benefit your team. Thank you for considering my application. I look forward to hearing from you.
Sincerely,
Poornima Kalal