Nikitha Kotian – This Molecular Biology Prodigy is Set to Change the Future of Genetic Research!

Nikitha Kotian

SUMMARY

A highly skilled and motivated Molecular Biology and Human Genetics student with a strong academic background and hands-on experience in research and laboratory techniques. Proficient in bioinformatics, biostatistics, and various laboratory skills. Seeking opportunities to further utilize and expand my knowledge in a challenging and rewarding environment.

EDUCATION

MSc (Molecular Biology and Human Genetics) | Aug 2022 – Jul 2024
Manipal School of Life Sciences, Manipal
CGPA: [hidden]

BSc (BZC) | Aug 2019 – Aug 2022
Dr. G Shankar Government Women’s First Grade College, Udupi
CGPA: [hidden]

SKILLS

• Laboratory skills: PCR, restriction digestion, RT-PCR, Isolation of plasmid DNA (miniprep, maxiprep), extraction of RNA, Bacterial cell transformation, Transfection, Gel preparation, gel electrophoresis, Animal handling (mice), pipetting, Lymphocyte culture, mammalian cell culture, HPLC, FTIR, TLC, column chromatography, synthesis
• Bio statistics: R programming basics, Prism – GraphPad and OriginLab
• Bioinformatics: multiple sequence alignment (BLAST, Clustal Omega, MEGA X), protein structure prediction and validation (SWISS-MODEL, Phyre2, PROCHECK), docking and ADME prediction (Schrodinger), primer designing (Primer3), NEBcutter
• MS Office: MS Word, MS Excel, MS Power Point

EXPERIENCE

Research student | 6 months

Project title: Synthesis and Evaluation of Novel HDAC/Topoisomerase Dual Inhibitors as Synergistic Chemotherapeutic Agents

• Synthesized R3P, a novel compound, achieving over 90% purity through a 5-step reaction process and purification techniques
• Conducted in silico analysis to evaluate target drug interactions and ADME properties, enhancing understanding of compound efficacy
• Maintained MCF7 and HeLa cell lines, performed MTT and colony formation assays to determine IC50 values
• Documented lab activities and developed Standard Operating Procedures (SOPs) to ensure reproducibility and compliance with protocols
• Presented findings through PowerPoint, effectively communicating complex concepts to peers and faculty
• Reported progress to PhD scholars and guides through presentations and discussions, addressing challenges and strategizing next steps
• Reviewed research for the thesis, created visuals with BioRender, and presented findings

ACTIVITIES

• Presented an e-poster at Manipal Quest [hidden], effectively communicating research to peers and faculty
• Presented research papers each semester, enhancing communication skills and effectively conveying complex ideas to diverse audiences
• Guided over 25 students during Science Day in navigating university resources, fostering interest in science and education
• Volunteered with the VSO, contributing to community outreach and promoting social responsibility

CERTIFICATES

• Fundamental Skills in Bioinformatics [Python, R]
• Whole genome sequencing of bacterial genomes – tools and applications

Keywords: Laboratory skills, Bio statistics, Bioinformatics, MS Office, Research, Communication

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Cover Letter:

I am writing to express my interest in the Genome Analyst position at MedGenome as advertised. With a Master’s degree in Molecular Biology and Human Genetics from Manipal School of Life Sciences and a strong foundation in bioinformatics, I am excited about the opportunity to contribute to your team in analyzing genomic data and advancing research initiatives.
During my academic journey, I have developed a robust skill set in bioinformatics, including multiple sequence alignment, protein structure prediction, and docking studies. My coursework in biostatistics and proficiency in R & Python programming will enable me to perform comprehensive data analyses and interpret results accurately.
I am eager to leverage my skills in genomic analysis to support your team’s objectives. I am confident that my background in molecular biology, combined with my passion for bioinformatics, will allow me to make a valuable contribution to your research efforts.
Thank you for considering my application. I look forward to the opportunity to discuss how my skills and experiences align with the goals of MedGenome.