**Manasvi Porwal**
Education
– B.Tech Biotechnology
Dr. D.Y. Patil Vidyapeeth, Pune.
Cumulative CGPA till 7th sem 8.68/10
– Higher Secondary Certificate
Art’s Science and Commerce College, Ahmednagar
Percentage – 86
– Secondary School Certificate
Kids Kingdom English High School, Aurangabad
Percentage – 90.20
Internship Project
**Bhabha Atomic Research Center, Mumbai**
Repurposing Antirheumatic Drug Auranofin for Acute Radiation Injury
– Investigated the therapeutic potential of Auranofin against radiation-induced hematopoietic damage.
– Hands-on experience in animal handling and Flow cytometry
– Explored molecular mechanisms of Auranofin, contributing to potential radioprotective strategies
**CSIR-Central Food Technological Research Institute, Mysuru**
Cloning of FTase gene in Pichia pastoris Strain
– PCR amplification, restriction digestion, ligation, and transformation into bacterial vectors (DH5α) and yeast expression system
– Induction of FTase expression under the AOX1 promoter using methanol in Pichia pastoris
– Multiple copy screening using Zeocin resistance
**Institute of Chemical Technology, Mumbai**
Extraction and application of xylanase enzyme
– Application of Biotechnology in Textile Industry
– Enzyme application in bleaching and dyeing
– Application, production, and Extraction of Xylan Enzyme in the Textile industry
Technical and Soft Skills Acquired
Certifications and Participation
– Course on Medically Important Bacteria (Phase 1)
– R Programming for Biologists
– MS-Excel Crash Course
– Attended Agritech theme-based event of NCL-OWOL campaign
– Handling of Model Organism – Mice, Drosophila
– Microbial Culturing
– Gene Cloning
– Cell Culture Techniques
– Protein Estimation and Induction
– Flow Cytometry
– Enzymatic assays
– Biosynthesis of Metabolites
– Molecular Biology Techniques
– French Language – A1
– Production of Nanoparticles
– Adaptable to diverse task environments
– Downstream processing of metabolites
– Basic Computational Analysis
Keywords
Biotechnology, Research, Internship, Biologist, Enzymes, Gene Cloning, Cell Culture, Protein Estimation, Flow Cytometry, Molecular Biology, French Language, Nanoparticles, Data Analysis, Microbial Culturing
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Cover Letter:
As a recent B.Tech graduate in Biotechnology, I am seeking an opportunity that will bridge the academic knowledge to the industrial aspect, leading to an impactful contribution in driving innovation. Quality Control Internship at Oracity Life Sciences aligns with one of the goals to get into the pharmaceutical industry, specifically in Quality Control and Assurance, where accuracy, scientific rigor, and regulatory compliance are paramount.
With a strong foundation, interest in core domains, and hands-on experience through different research internships, I transformed from a student with minimal research experience to a budding biotechnologist, equipped with the ability to approach problems with a researcher’s mindset. During my academic and internship tenure, I have gained practical experience in molecular biology techniques, flow cytometry, data analysis, and sample documentation-all within research-intensive, protocol-driven environments. With a willingness to learn and contribute, I would like to join Oracity’s team and gain exposure to the pharmaceutical industry across R&D, Quality Assurance, and Quality Control departments. I am interested in this role because of the structured training it offers and the potential to transition into a long-term position. I see this internship not just as a stepping stone but as a critical foundation to build a focused and impactful career in pharmaceutical quality systems.
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