Lakshmi P V – Unlocking the Secrets of Biotechnology and Optometry for Revolutionary Drug Development!

PROFESSIONAL SUMMARY

Biotechnology postgraduate with expertise in molecular biology, CRISPR-Cas genome editing, and analytical techniques.

Passionate about drug discovery, biopharmaceutical research, and high-throughput screening.

Proficient in HPLC, Mass Spectrometry, GMP/GLP compliance, and bioinformatics tools.

Strong analytical and problem-solving skills, with hands-on experience in designing experiments, data analysis, and regulatory compliance.

Eager to apply laboratory and analytical expertise in a dynamic pharmaceutical or research environment, contributing to drug development, process optimization, and regulatory excellence.

Trained in optometry with experience in patient care, diagnostic testing, and clinical research, enabling a unique blend of technical and clinical skills.

EDUCATION

Master of Science (Biotechnology) | 2023–2025 | Amrita School of Biotechnology, Kerala

Relevant Coursework: Molecular Biology, Genetic Engineering, Pharmacology, Analytical Techniques

Bachelor of Science (Biotechnology) | 2020–2023 | Hindustan College of Arts and Science, Tamil Nadu

Diploma in Optometry | 2020–2023 | Hindustan College of Allied Sciences, Tamil Nadu

RESEARCH & INDUSTRY EXPERIENCE

Project Intern | MS Swaminathan Research Foundation (Jan 2025–May 2025)

Responsibilities:

• Conducted genome editing experiments using CRISPR-Cas9 for abiotic stress tolerance in rice.
• Analyzed data using bioinformatics tools and statistical methods to validate gene-editing efficiency.
• Maintained detailed records of experiments and results.

Final Year Research Project | Phenotypic Evaluation of Rice Accessions for Salinity Tolerance Under Hydroponics (2023)

Tasks:

• Evaluated 95 rice accessions using molecular markers and physiological traits.
• Identified 3 accessions with enhanced salinity tolerance, validated via qRT-PCR.
• Designed and implemented hydroponic screening protocols for salinity stress assessment.

Intern – Clinical & Analytical Experience | Smart Vision Clinic (2021–2023)

Responsibilities:

• Conducted eye tests, refraction, and retinoscopy for patient evaluations.
• Assisted in clinical research on vision-related disorders and optometry techniques.
• Provided patient education on vision care and contact lens use.

SKILLS

Biotechnology Skills

• Molecular Techniques: PCR, Gel Electrophoresis, SDS-PAGE, CRISPR-Cas9, qRT-PCR, DNA & RNA isolation, Blood Grouping, Agglutination, Cell staining, ELISA, Cell Counting, Western blotting.
• Analytical Techniques: Chromatography (HPLC, GC), Spectroscopy (UV-Vis, Mass Spectrometry), Mass Spectrometry: ESI, EI, MALDI-TOF, CI, Proteomics: PMF, TANDEM MS/MS, ICAT, iTRAQ.
• Bioinformatics & Computational Biology: BLAST, Clustal Omega, AutoDock, Schrödinger Suite, RNA-Seq, PCR.
• Regulatory & Quality Compliance: GMP, GLP, ICH Guidelines, FDA Regulations, SOP Development.
• Data Analysis & Programming: Python for Biologists, R for Biostatistics, MS Office, Machine Learning (basic).
• Laboratory Management: Inventory management, sample preparation, equipment calibration.
• Biochemistry tests: Purification of Protein, Standard Curve, Specific activity, Phytochemistry.
• Isolation of microorganisms, Sulfite oxidation method, Immobilisation of yeast cells, Enzyme activity, Bacterial staining, Biochemical Tests.

Optometry Skills

• Diagnostic Testing: Refraction, Retinoscopy, Tonometry, Visual Acuity, Slit Lamp Biomicroscopy.
• Patient Care: Comprehensive eye exams, diagnosis of refractive errors, glaucoma screening, diabetic retinopathy assessment.
• Clinical Procedures: Contact lens fitting, vision therapy, presbyopia management, fundus photography.
• Patient Education: Explaining vision care, lens use, and eye health management, Patient Handling & Communication, Taking case history, Counseling patients about vision care and eyewear, Explaining test results simply and clearly.
• Medical Documentation: Maintaining patient records, EMR proficiency, insurance processing.
• Dispensing Optics: Lens types (single vision, bifocal, progressive), Frame selection and fitting, Measurements for pupillary distance (PD), segment height.
• Ocular Anatomy & Physiology, Understanding structure and function of eye parts, Eye diseases basics (cataract, glaucoma, diabetic retinopathy).

Additional Skills

• Critical Thinking & Problem Solving: Analytical approach to troubleshooting and decision-making.
• Scientific & Technical Writing: Clear documentation of experiments and clinical findings.
• Time Management & Project Coordination: Efficiently managing multiple tasks and deadlines.
• Teamwork & Collaboration: Working effectively in multidisciplinary teams.
• Communication: Fluent in English, Tamil (Native), and Hindi (Fluent).

CERTIFICATIONS & TRAINING

NPTEL – Environmental Biotechnology (2021–2022)

NPTEL – Cellular Processes & Organization (2022–2023)

PROJECTS & WORKSHOPS

Workshops & Webinars:

• EDII-TN & University of Madras – E-leader & Design Thinking Workshop
• Attended webinars on CRISPR applications, pharmacogenomics, and drug development.

KEYWORDS (SKILLS)

Molecular Biology, CRISPR-Cas9, HPLC, Mass Spectrometry, Bioinformatics, GMP, GLP, Data Analysis, Optometry, Patient Care, Clinical Research, Diagnostic Testing, Vision Care, Teamwork, Communication, Regulatory Compliance.

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Cover Letter:

Lakshmi P.V
Chennai, Tamil Nadu

Date: 20/06/2025

HR Manager
Kwality Pharmaceuticals Ltd
Amritsar, Punjab

Subject: Application for the Post of Microbiology Executive

Dear Sir/Madam,

I am writing to express my keen interest in the position of Microbiology Executive at Kwality Pharmaceuticals Ltd, as advertised. With over one year of hands-on experience in microbiological techniques, quality control, and sterile environment practices, I am confident in my ability to contribute effectively to your QA/QC and R&D teams.

I hold a Diploma in Optometry (2021–2023) from Hindustan College of Allied Sciences and have completed a one-year internship at Smart Vision Clinic, where I developed strong laboratory handling skills, patient care practices, and infection control awareness. During my tenure, I gained experience in sterile techniques, media preparation, microbial testing, and report documentation—all of which align with the requirements of a pharmaceutical microbiology role.

Key highlights of my qualifications include:

Practical knowledge of Good Laboratory Practices (GLP) and Good Manufacturing Practices (GMP)

Understanding of microbiological testing methods including bioburden, endotoxin, and environmental monitoring

Ability to work collaboratively in cross-functional teams and ensure compliance with SOPs and regulatory standards

I am deeply motivated to join Kwality Pharmaceuticals Ltd, a company known for its commitment to excellence and innovation in the pharmaceutical sector. I am confident that my academic background, along with my laboratory and documentation skills, make me a suitable candidate for this role.

I have attached my resume for your review. I would welcome the opportunity to discuss how I can contribute to your team. Thank you for considering my application.

Yours sincerely,
Lakshmi P.V