Jyoti Bakshi – Unveiling the Secrets of Biotechnology: How This Ph.D. Researcher is Revolutionizing Drug Development

• Kurukshetra University
• Plant Tissue Culture: Training cum project on plant Commiphora wightii.
• Performed invitro propagation, multiplication and conservation of the endangered plant Commiphora wightii by using various vegetative explants.

Trainee

• Biowits Life Science Pvt. Ltd.
• Worked on Project named ‘Antimicrobial activity of Neem extract against soil microorganism’.
• Performed disc diffusion and well diffusion method and learned techniques such as Media preparation and sterilization, DNA isolation from Blood samples and bacterial samples and performed electrophoresis, and PCR.

Education

• Ph.D. Biotechnology, Guru Jambheshwar University of Science & Technology
• M.Sc Biotechnology, Kurukshetra University
• B.Sc Biotechnology, D.A.V. Girls college
• Certificate of Higher Education, D.A.V. Public School

Awards

University Topper in Masters of Science

Publications

Exploring the antimicrobial potential of Hesperetin: A study on clinically relevant bacterial and fungal pathogens. Assim Verma, Jyoti Bakshi, Garvit Kumar, Himanshu Kamboj, Rekha Verma, Pooja Vijayaraghavan, Aman Singh, Naveen Kumar, Sanjay Barua and Santosh Kumari. J. Pure Appl. Microbiol.

Potentiation and in vivo evaluation of antiobesity activity of berberine through encapsulation in guar-acacia gum nanocomplexes. Bakshi, J., Lather, P., Verma, A., Lather, D., Grewal, S., Dhingra, D., Kumari, S.

Sodium alginate polymer nanoformulation as promising carrier for berberine delivery: Synthesis, morphology and in-vitro evaluation. Mehra, M., Sheorain, J., Bakshi, J., Grewal, S., Dhingra, D., Bernela, M., Kumari, S.

Improving the biological properties of berberine using gellan and gum acacia as a potential delivery carrier. Bakshi, J., Grewal, S., Dhingra, D., Kumari, S.

Synthesis and evaluation of berberine loaded chitosan nanocarrier for enhanced in vitro antioxidant and anti-inflammatory potential. Mehra M., Sheorain J., Bakshi J., Thakur R., Grewal S., Dhingra D., Kumari S.

Synthesis, Characterization and Evaluation of in vitro antimicrobial and anti-diabetic activity of berberine encapsulated in guar-acacia gum nanocomplexes. Bakshi, J., Mehra, M., Grewal, S., Dhingra, D., Kumari, S.

Berberine Loaded Tragacanth-Acacia Gum Nanocomplexes: Synthesis, Characterization and Evaluation of in vitro Anti-inflammatory and Antioxidant Activity. Bakshi, J., Mehra, M., Grewal, S., Kumari, S.

Evaluation of anti-inflammatory response of berberine-loaded gum nanocomplexes in carrageenan-induced acute paw edema in rats. Bakshi, J., Lathar, P., Mehra, M, Grewal, S., Kumari, S.

Skills

Molecular biology, DNA and RNA isolation, Agarose gel electrophoresis, ELISA, Polymerase Chain Reaction (PCR), Quantitative PCR (qPCR), Competent cell preparation, Bacterial cell transformation, Restriction Digestion, Ligation, Recombinase Polymerase Assay (RPA), SDS-PAGE, Purification of protein, Animal handling (mice, rats) and Experimentation, Softwares: GraphPad Prism, Origin, Design Expert, INSTA, Statistical Analysis of data, Ability to work independently or on team-oriented projects, Preparation of Drug Nanoformulations, Microbiology, Bacterial isolation and characterization (manual biochemical testing and API kit), Antibiotic Susceptibility testing (AST), Preparation of different media and biochemicals, Agar well diffusion and disc diffusion, Maintenance and Preservation of bacterial isolates, MS word, Excel, Power point, Quick learner and good presenter, Friendly and Positive Attitude

Cover Letter:

My name is Jyoti Bakshi and I am reaching out to express my interest at your organization. As a researcher with over 5 years of research experience, I have gained experience, expertise and knowledge needed to perform high-quality research in the biotechnology and nanotechnology fields. I believe my strong background in nanotechnology and biotechnology; and laboratory equipment, most importantly, needed in different areas of biotechnology. Area of interest: Nanoparticles, Bacteriology, Drug delivery, Molecular biology, cytogenetics My PhD thesis was focused on herbal drug nanoformulations using natural gums. The aim of the work was to improve the biological potential of drug _via _improving bio-availability. The prepared nanoformulations were evaluated using various _in vitro _biological activities such as antimicrobial, antioxidant, anti-diabetic and anti inflammatory. In addition, the anti-inflammatory and anti-obesity activities of the prepared formulation were also evaluated _in vivo_ on carrageenan induced rat paw edema model and high fat diet induced obese rat model, respectively. The expression of genes involved during _in vivo_ anti-inflammatory study was also analyzed using quantitative real time PCR (qRT-PCR). I believe that my previous work experiences, coupled with my extensive knowledge of bacteriology, nanotechnology and biotechnology techniques, would be a great fit for any position or any intership position in your organization. I have approx 1 yr 7 month experience in a bacteriology lab. I look forward to applying the skills I have learned and learning towards this position. My future goals are to expand my research area into bacteriology, drug delivery, cell culture, and various molecular biology techniques, and to learn new techniques to understand, diagnose, and treat diseases.