CURRICULUM VITAE
John Doe
City, State
Professional Summary:
A dedicated and results-driven professional with over 5 years of experience in marketing and project management. Strong analytical skills combined with a creative mindset to develop effective marketing strategies. Proven track record of successfully leading cross-functional teams and delivering projects within deadline and budget constraints.
Experience:
Marketing Manager, XYZ Company
City, State
Date – Present
Responsibilities:
- Developed and implemented comprehensive marketing strategies to increase brand visibility and drive sales.
- Managed digital marketing campaigns across various platforms to engage target audiences.
- Analyzed market trends and competitor activities to identify opportunities for growth.
Education:
Bachelor of Business Administration
University Name, City, State
Date of Graduation
Skills:
Marketing Strategy, Project Management, Digital Marketing, Market Analysis, Team Leadership
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Cover Letter:
Dear Admission Committee,
I hope you are doing well. My name is **Renuka Mulik**. I am writing to express my sincere interest in applying for the **Scientist – Molecular Biology** position at D-NOME Biosciences in Hyderabad.
I am particularly drawn to your lab’s studies that focus on bioscience. The opportunity to investigate molecular biology that strongly aligns with my academic training and research interests. I am deeply motivated by your work. My undergraduate in Chemistry at Shivaji University provided a strong foundation in organic and biochemical mechanisms, particularly in enzyme function and molecular analysis techniques such as mass spectroscopy and chromatography—skills that are highly relevant in translational research. To deepen my understanding of life sciences, I pursued a Master’s in Microbiology at Krishna Institute of Medical Sciences in 2020.
Further, I also gained wet lab hands-on training by attending summer research camps and conferences that helped to foster curiosity and scientific undertakings, For instance, I took a wet lab training summer internship at IISC Bangalore, affiliated lab Protein design under Dr Deepti Saini through which I grain knowledge of Primer designing using BLAST, molecular cloning, restriction digestion in C41 Bacterial plasmids and Identification pET28a, pET24a expression plasmids and, I completed a two-week wet lab training workshop at the National Brain Research Centre in Haryana, where I earned certification in Laboratory Animal Science (CCLAS). I was trained in handling rodents, B form filling, animal welfare guidelines, 3Rs in research and conducting behavioural studies, including the administration of saline through renal, oral gavage, intraperitoneal, intramuscular, and intravenous injections. I also learned to induce Parkinson’s disease models via stereotactic surgery using 6-OHDA. Also, I gave an oral presentation titled, “Investigation of the effect of semaglutide in MPTP-induced Parkinson’s disease in mice via oral gavage” The study of neuroinflammation, along with cell signalling processes in molecular pathways, drives my interest in rodent-based translational research. I developed not only technical skills but also a strong appreciation for collaboration.
During my Master’s thesis, I investigated the gut microbiota of Tenebrio molitor, focusing on bacteria capable of degrading polystyrene. This work enhanced my laboratory skills and deepened my understanding of plastivore microbiology, while also sparking my interest in the gut-brain axis of interdisciplinary research. My curiosity brought me to the Centre for Neuroscience in Kochi, Kerala, in 2023, where I worked as a Project Assistant under Dr Baby Chakrapani P.S. At the Centre, I used a C57BL/6 mouse model to investigate how colitis affects the connection between the brain and gut. I researched how the gut microbiota and the brain interact bidirectionally, and faecal microbiota transplantation can mitigate IBD conditions. Also, research on how bile acids trigger cytotoxic stress and signalling pathways. I worked on several molecular biology methods, like Western blotting, SDS-PAGE, extracting RNA with TRIzol, RT-PCR, qPCR, FACS, immunohistochemistry, gel electrophoresis, and MTT assays.
As I investigated colitis-induced ER stress, I have learned more about the UPR, oxidative stress, mitochondrial dysfunction, and neuroinflammation, and how they are involved in the function of the neuroimmune system. This experience has improved my research abilities, especially with molecular techniques, data analysis (T-test ), and projects that involve generating and testing a hypothesis.
Most recently, I conducted research under Dr. Mustak on the neuroprotective potential of phytochemicals in an epileptic mutant Drosophila model. The project focused on identifying compounds such as quercetin to reduce inflammation and programmed cell death, particularly by suppressing NF-κB activity and reducing reactive oxygen species (ROS). Using climbing assays and molecular techniques, I investigate how antioxidant-rich phytochemicals exhibit anti-inflammatory effects and their potential as treatments for seizures. During this time, I also followed up on a related project previously initiated by PhD alumni: “Ionising Radiation-Induced ER Stress Response in Astrocytes and Neuronal Cell Neuroprotection by Phytochemicals (in vivo and in vitro).” This study examined how ionising radiation (IR) activates the unfolded protein response (UPR) via BIP and CHOP expression in astrocytes, leading to cellular damage and cognitive decline. These findings suggest that targeting the UPR cascade may mitigate radiation-induced neurodegeneration. Through this work, I have developed a deep appreciation for the attention to detail, discipline, and perseverance required in a research setting.
Through my training, I have gained substantial experience in cellular and molecular biology, and I am confident that I am a strong candidate for this position, as I have adequate knowledge of rodent behavioural experiments, molecular techniques, and translational animal modelling. Further, I am eager to refine my knowledge under your mentorship. D-NOME biosciences, with its renowned translational research program, research facility, and collaborative research environment, offers the ideal setting to grow as an independent scientist. My long-term goal is to establish a research lab focused on developing diagnostic and therapeutic strategies, and I believe that D-NOME biosciences will provide the mentorship and scientific environment necessary to realise this vision.
What draws me most to this opportunity at D-NOME Biosciences is the chance to apply and expand my expertise in **recombinant protein expression, purification, and assay development** within a cutting-edge translational research environment. I am particularly excited about working with bacterial expression systems and chromatographic purification to generate high-quality proteins for downstream applications. These research programs—rooted in academic exposure and rigorous mentorship—reflect the kind of environment in which I hope to thrive. My prior research experiences, paired with a strong sense of curiosity and dedication, have prepared me to contribute meaningfully to such a setting, applying creative problem-solving techniques to address research questions.
I am thankful to the admission committee for their review of my application, and I am looking forward to hearing from you.
Sincerely, Renuka Mulik
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