Himani Negi

Research Experience

• Project Scientist I (January 2025- Present) at ICGEB, New Delhi, on a project entitled “Targeting drug resistance in tuberculosis and malaria.”
• Investigated the uptake reduction of selected scFvs in THP-1 monocyte-derived macrophages
• Evaluated the efficacy of selected scFvs in animal model (mice)
• Project Associate II (January 2024- December 2024) at ICGEB, New Delhi, on a project entitled “Targeting drug resistance in tuberculosis and malaria.”
• Developed and characterized novel therapeutic scFvs against Mycobacterium tuberculosis surface protein
• Screened Tomlinson I, J and HuScL-4 phage-displayed human scFv libraries against Mtb surface protein

Education

• MTech. Biotechnology (2021-2023) Sharda University, Greater Noida, Uttar Pradesh, India (CGPA= 8.5)
• BTech. Biotechnology (2015-2019) Manav Rachana International institute of Research and studies, Faridabad, Haryana, India (CGPA= 6.86)

Professional Skills

• Molecular Biology, Cell Culture, Protein Science, Immunology, Bioinformatics, Data analysis

Soft Skills

• Problem solving, Time-Management, Critical thinking, Work ethics, Teamwork, Active listening

Publications

• Aynalem, B., Negi, H., Alemu, Y., Sehrawat, N. & Kumar, A. (2024). Citric acid: fermentative production using organic wastes as feedstocks. Physical Sciences Reviews, 9(9), 3043-3055.
• Passricha, N., Saifi, S.K., Negi, H., Tuteja, R., & Tuteja, N. (2020), Chapter 20 – Transgenic approach in crop improvement, Advancement in Crop Improvement Techniques, Woodhead Publishing, 2020, Pages 329-350
• Passricha, N., Saifi, S.K., Negi, H. and Tuteja, N. (2020). Lectin Receptor like Kinase. In Protein Kinases and Stress Signaling in Plants, G.K. Pandey (Ed.)

Poster Presentations

• Himani Negi, Dhrubayoti Mahata, Eirene Prasad, Amit Kumar, Ujjwal Kumar, Dhiraj Kumar and Dinakar M Salunke (Target Specific Antibodies Against Surface Proteins of Mycobacterium tuberculosis (Mtb))
• Esha Kumar, Himani Negi, Nishat Passricha, and Dhiraj Kumar (High Throughput Gateway Cloning for Selected Human Genes)

Certificates

• Hands-on training Workshop attended on qPCR instrument
• Attended the 4th plant proteomics workshop on “Sea buckthorn-Genomics, Proteomics & Metabolomics”

Languages

• English, Hindi, German

References

• Dr. Dinakar M. Salunke: [Email Hidden]
• Dr. Dhiraj Kumar: [Email Hidden]

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Cover Letter:

I am writing to express my keen interest in applying for the post of Scientist-Molecular Biology.
I am working as Project Scientist at International Center for Genetic Engineering and Biotechnology (ICGEB)- New Delhi, currently working on a project titled entitled “Targeting drug resistance in tuberculosis and malaria”.
Here, my role is to develop and characterize novel therapeutic scFvs against targeting _Mycobacterium tuberculosis (Mtb)_ surface protein using phage-displayed scFv libraries.
I have successfully screened Tomlinson I, J and HuScL-4 phage-displayed human scFv libraries against _Mtb_ surface protein and their epitope to develop potential _Mtb_ neutralizing scFvs followed by cloning and purifying recombinant proteins at a milligram scale, and evaluated their binding affinity using ELISA, Biolayer Interferometry (BLI) and Flow cytometry. Further investigate the uptake reduction of selected scFvs in THP-1 monocyte-derived macrophages. Subsequently, evaluate the efficacy of selected scFvs in animal model (mice) and engineer them into alternative formats, including scFv-Fc fusion proteins and full-length monoclonal antibodies (mAbs), for further functional characterization.
I completed my M.Tech Biotechnology specialization in Genetic Engineering from Sharda University, Greater Noida in June 2023. During my Master’s training period, I have done my dissertation project in Genomics and Integrative Biology (CSIR–IGIB) project entitled “Construction of CMPK2 Deletion Mutants and Validation of its Expression in HEK293T cells” where my responsibilities helped me to gain experience in Molecular biology and Cell and Tissue culture techniques. During my Bachelor’s training period, I took up my B.tech. final year project in the molecular biology and genomics Lab at the International Centre for Genetic Engineering, New Delhi under the supervision of Dr. Dhiraj Verma. I completed my thesis entitled “High throughput Cloning of Human and _Mtb_ gene”. This project has helped me gain research experience in Basic molecular and microbiological techniques. I must tickle my mind to solve and troubleshoot problems and analyze the results at this level. These works helped me have a deep understanding and knowledge of genomics and molecular Biology.
I have experience of working in a startup company Biologia Research India Pvt. Ltd. (2019-2022), As the works were multidisciplinary and based on the types of samples received. So had to proceed as well as design the experiments accordingly. As a result, I have inculcated good expertise in the molecular Biology techniques (Cloning, DNA Barcoding, DNA isolation, RNA isolation, bacterial Transformation, plasmid Isolation, PCR, qRT-PCR, small scale protein expression, SDS PAGE, and western blotting).
Joining your organization will be a great opportunity for me to grow professionally in an innovative environment and broaden the horizon of my scientific understanding. I believe I will be a good fit in the group due to my previous experience in molecular biology, protein biochemistry, and my curiosity to explore new ventures. During my working period, I have learned not only to be an independent researcher but also to be creative. I can manage and thrive in stressful situations and have worked as part of a team and individually. I am optimistic that with my independent and team skills, I can directly contribute to the organization.
I would be happy to answer should you require any further information.
I am looking forward to hearing from you.