Gunjan Sharma – Uncovering the Secrets of Mosquito Resistance: A Ph.D. Journey in Life Sciences

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Gunjan Sharma, [hidden]

Introduction Summary: Gunjan Sharma, [hidden], an Indian national, is a dedicated Life Sciences researcher with expertise in molecular biology, particularly focusing on the role of gene duplications in insecticide resistance in mosquitoes. Gunjan has a strong academic background and a proven track record of research projects and publications in the field.

Research Skills:

  • Analytical Skills
  • Grant writing
  • Report writing and Literature Mining
  • Research Planning and Laboratory Operations
  • Proficient Knowledge of Microsoft Word and Microsoft Excel
  • Complete familiarity with MS PowerPoint
  • Good communication and presentation skills
  • Ability to lead projects and teams

Molecular Biology Expertise:

  • DNA/RNA isolation and quantification
  • cDNA library preparation
  • Conventional and Real-time PCR
  • Agarose gel electrophoresis
  • DNA Sanger sequencing
  • DNA and cDNA Cloning
  • Gene silencing (RNAi)

Projects Undertaken:

Project Title Duration/Year University/Institute
Bioinformatics Winter Internship – Global Research Tools & Techniques 6 Months (Ongoing) 2024 BioTecNika
Structural mapping of global sequence variations in Plasmodium vivax Duffy binding protein-II 6 months (2013) [hidden] Dissertation (ICMR-NIMR)
A Database of Simple Sequence Repeats of Aspergillus Fumigatus 6 Months (2012) During [hidden] (Internal Project)

Academic Record:

Qualification Passing Year Subject(s) Board/University/Institute Percentage
Matric 2006 Science, Maths, Hindi, English, Social Studies St. Mary’s Convent School (CBSE) 64%
HSC 2008 Physics, Chemistry, Biology and Physical Education Doon Global School (CBSE) 61%
Graduation 2011 Computer applications and Statistics, Biochemistry, Bioinformatics Mukand Lal National College – [MLNC], Yamuna Nagar, Kurukshetra University 66%
Post-Graduation 2013 Molecular Phylogeny, Project Dissertation Banasthali Vidyapith 69%
[hidden] 2024 Research Methodology, Biostatistics & computer applications in biological research, Insect Pest Management Indira Gandhi National Open University 69%

Work Experience/Research Experience:

Designation Employer From Date To Date Project Title Publications
Project Fellow ICMR-National Institute of Malaria Research 22-11-2013 25-9-2015 Molecular and morphological characterization of Minimus group of mosquitoes in Northeastern states and bordering areas (DRL) 1
Senior Project Fellow ICMR-National Institute of Malaria Research 2-09-2016 30-12-2016 Molecular population genetic analysis of Anopheles Fluviatilis (ICMR) 0
Research Assistant ICMR-National Institute of Malaria Research 30-3-2017 30-3-2018 Specific bionomic characters of malaria vectors and their sibling species in Haryana (ICMR) 1
Senior Project Fellow ICMR-National Institute of Malaria Research 16-8-2018 15-7-2022 Exploring insecticide-resistance associated alterations in Acetylcholinesterase (AchE) genes (mutations and gene duplications) of Anopheles stephensi and Aedes aegypti from New Delhi (ICMR) First Draft ready

Research Publications:

  • Singh, O. P., Dykes, C. L., Sharma, G., & Das, M. K. (2015). – Journal of medical entomology, 52(1), 24–27.
  • Singh H, Singh OP, Akhtar N, Sharma G, Sindhania A, Gupta N, Valecha N. – Acta Trop. 2019 Nov; 199:105114.
  • Sharma, G, Lather, M, & Singh, O. P. – Indian Journal of Experimental Biology (IJEB), 2022; 58(01), 64-68.
  • Sindhania A, Das MK, Sharma G, Surendran SN, Kaushal BR, Lohani HP, Singh OP. – Malar J. 2020 Nov 19;19(1):417.

Awards received:

  • Received Senior Research Fellowship (SRF) from ICMR during 2018.
  • Appreciation letter from ICMR Prof. (Dr.) Balram Bhargava for zika virus detection in mosquitoes.
  • Travel fund award from ICMR (2017) and CSIR (2018) to attend international Conference ASTMH in New Orleans, Louisiana USA, 2018.

Other achievements:

  • Completed certificate course in Radio Production (RJ-ing and Anchoring) conducted by Banasthali vidyapith University in 2012 with A grade.
  • Merit certificate in District Mathematics OLYMPIAD-2000 organised by New Era Foundation (NEFS).

Conferences Attended:

  • Presented Poster at ASTMH, 2018.
  • Oral Presentation at SOVE, 2019.
  • Presented Poster at ISMOCD, 2019.
  • Presented Poster at “World Malaria Day”, 2019.
  • Attended ICB-09 and National Conference on Biochemistry.

Trainings Attended:

  • Attended training on Foldscope, Department of Biotechnology, Govt. of India, 2019.
  • Attended training on Basic Entomology at ICMR-NIMR, New Delhi, 2021.
  • Completed training on C, C++ and core java.

Skills: Analytical Skills, Grant writing, Report writing, Literature Mining, Research Planning, Laboratory Operations, Microsoft Word, Microsoft Excel, MS PowerPoint, Communication, Presentation, Molecular Biology, DNA/RNA isolation, PCR, Gel electrophoresis, Sequencing, Cloning, Gene silencing.

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Cover Letter:

11 February, 2025
Dear Sir/Mam,
I am writing to apply for a position in your laboratory and would like to continue my career in your laboratory.
I have strong educational background having earned my [hidden] in Bioinformatics from Kurukshetra University, followed by an [hidden] in Bioinformatics from Banasthali Vidyapith. Additionally, I have completed my [hidden] in December 2023 under the supervision of Dr. [hidden] Singh, Retired Scientist ‘G’, Vector biology division, ICMR-NIMR, New Delhi. I have joined ICMR-National Institute of Malaria Research in the year 2013. I have done exceedingly well with my research assignments and has total of 10 publications out of which one being the first author from my PhD work. Additionally, I have pursued an appreciation letter from ICMR Prof. (Dr.) Balram Bhargava (Director-General, ICMR) for the zika virus detection in mosquitoes.
In my recent research endeavours, I have been deeply involved in the field of genomics, specifically whole genome sequencing to unravel the intricate mechanisms underlying genetic variation within _Anopheles stephensi and Anopheles culicifacies _in [hidden] My work has centred around elucidating the significance of gene duplications and structural variations in shaping genomic landscapes and influencing phenotypic traits. I have proficiently handled a range of molecular biology techniques, including Cloning, Real-time PCR, and Sanger Sequencing, as well as possessing familiarity with various bioinformatics tools. I believe these skills will contribute significantly in your lab.
Given the opportunity, I am confident that I will be able to contribute successfully to your ongoing projects that I will need to succeed in my future independent research endeavours.
I have enclosed a copy of my curriculum vitae. If you require additional information, please contact me via email. Thank you for considering my application. I am looking forward to the possibility of discussing how my skills and experiences align with the requirements of the position.
 
Sincerely,
Gunjan Sharma (Ph. D.)

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