Ph.D. Biotechnology | MSc, M.Phil
📍 Bangalore, India
Professional Summary
Experienced Molecular Biologist with a Ph.D. in Biotechnology and over 6 years of applied research and teaching in molecular cloning, gene expression, and mammalian cell culture. Proven expertise in cell line development, vector construction, transient and stable gene expression, and protein characterization techniques such as ELISA, SDS-PAGE, Western blot, and HPLC. Adept at using bacterial and yeast expression systems. Demonstrated proficiency in SOP/GMP-compliant documentation, single-cell cloning, and assay development for industrial and academic research.
Core Technical Competencies
- Molecular Cloning & DNA Techniques: Plasmid/genomic DNA isolation, PCR, restriction digestion, gel extraction, ligation, sequencing, and confirmation.
- Gene Expression Systems: Experience with both stable and transient transfections in mammalian cells; vector construction and assay optimization.
- Protein Characterization: SDS-PAGE, Western blotting, ELISA, HPLC, and Octet-based binding assays.
- Cell Line Development: Cell banking, single-cell cloning, fed-batch culture, monoclonality assurance, viability assays.
- Microbial Systems: Skilled in bacterial and yeast expression systems for recombinant protein expression.
- Cell Culture: Mammalian cell thawing, subculturing, media optimization, and transfection.
- Documentation & Compliance: Preparation of SOPs, batch records, GMP documentation, and electronic lab notebooks.
- Analytical Skills: qRT-PCR, spectrophotometry, protein quantification, biostatistical analysis.
Professional Experience
Assistant Professor / Scientist
Dept. of Life Sciences, Indian Academy Degree College – Bangalore
📅 June 2024 – Present
- Designed and executed vector construction strategies for gene expression in mammalian cells.
- Performed stable and transient transfection, monitored expression using protein quantification methods (ELISA, Western Blotting).
- Supervised monoclonality assays and established single-cell derived stable lines.
- Maintained SOPs and project data in electronic laboratory notebooks.
Education
Ph.D. in Biotechnology, CSIR-CFTRI, University of Mysore – 2015
M.Phil. in Biochemistry, University of Kerala – 2009
M.Sc. in Biochemistry, Bangalore University – 2006
Certifications & Training
- Certificate in “Mass Spectrometry-Based Proteomics” – 2021
- “Microarray Certification program”-2025
Key Achievements
- Developed recombinant plasmids for enzyme expression and protein purification.
- Successfully managed multiple mammalian cell line projects with monoclonal selection.
- Delivered 12 filed patents and 16 peer-reviewed scientific publications.
- Recognized with National Postdoctoral Fellowship – DST-SERB (2016).
Publications & Patents
- 12 Patents Filed
- 16 Peer-Reviewed Publications
- 5 Book Chapters Published
Soft Skills & Industry Alignment
- Excellent multitasking and timeline management in regulated lab settings.
- Strong communicator with proven ability to lead research and mentor teams.
- Adaptable and analytical thinker, able to troubleshoot experimental issues quickly.
Skills
Molecular Cloning, Gene Expression, Cell Line Development, Protein Characterization, Cell Culture, Documentation, Analytical Skills
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Cover Letter:
I am writing to express my keen interest in the position of scientist in your esteemed company. With a Ph.D. in Biotechnology from CSIR-CFTRI, Mysore, and over **7.7 years of postdoctoral research experience**, I am passionate about advancing cutting-edge research and fostering interdisciplinary collaborations in molecular biology, bioinformatics, and biotechnology.
My research experience spans **RNA editing site prediction in Alzheimer’s disease using machine learning, cancer molecular biology, genetic engineering, and biotherapeutics**. As a **recipient of prestigious fellowships** such as the **SERB National Postdoctoral Fellowship (INR 9,60,000 per annum) and DBT-RA Research Associateship**, I have successfully led independent research projects, secured funding, and contributed to the scientific community with **16 peer-reviewed publications, 12 patents, and 5 book chapters**. I have also actively participated in organizing conferences and mentoring students in research.
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