Dr. M. ANGEL NIVYA

Indian nationality

Summary

A dedicated and experienced researcher with a [hidden] in Biotechnology and a strong background in protein purification, molecular biology, and biophysical techniques. Proficient in conducting research and publishing findings in reputable journals.

Professional Experience

Project Scientist II (March 2024 – June 2024)
Translational Health Science and Technology Institute (THSTI), Faridabad, India

Research Associate (April 2023 – February 2024)
Institute of Genomics and Integrative Biology (IGIB), New Delhi, India

Research Associate 1 (March 2019 – April 2022)
Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India

Education

[hidden] in Biotechnology, 2014 – 2018
Indian Institute of Technology Hyderabad (IITH), Hyderabad, India

[hidden] in Biotechnology, 2009 – 2011
St. Joseph’s College, Tiruchirappalli, Tamilnadu, India

[hidden] in Biotechnology, 2006 – 2009
[hidden] Arts College, Thanjavur, Tamilnadu, India

Skills

Protein Skills: Cloning, Expression, and Purification of Proteins, SDS-PAGE, Western blot, Bioinformatics

Molecular Biology Skills: Plasmid preparation, Transformation, PCR, DNA sequencing, RT-PCR

Biophysical Techniques: Fluorescence Polarization, Isothermal Titration Calorimetry

Computational Skills: Molecular Modelling, Docking, Simulation, Sequence Analysis

Research Experience

Peptides based Drugs: Synthesis of Peptides at Indian Institute of Science

Plant Molecular Biology: Trained at the Rajiv Gandhi Center for Biotechnology

CoVID19: Involved in Cloning and Expression of Proteins

Publications

• Tanneru N., Nivya MA., et al. (2023). Plasmodium DDI1 – International Journal for Parasitology.
• Sudhakar R., Nivya MA., et al. (2022). Antibody response in SARS-CoV-2-infected individuals – Indian Journal Medical Research.

References

Prof. Rajakumara Eerappa
Professor and HOD
Dept. of Biotechnology, IIT Hyderabad
Email: [hidden]

Prof. Puran Singh Sijwali
Sr. Principal Scientist
Centre for Cellular and Molecular Biology (CCMB)
Email: [hidden]

Prof. Archana Singh
Principal Scientist
Institute of Genomics and Integrative Biology (IGIB)
Email: [hidden]

Prof. Milan Surjit
Professor
Translational Health science and Technology Institute (THSTI)
Email: [hidden]

Honors & Awards

3rd position in 10th Class
Qualified Graduate Aptitude Test in Engineering (GATE-2014)

Work Style

Strong independent work style with excellent team work skills. Passionate and well-organized.

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Cover Letter:

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Dear Sir,
I am M. Angel Nivya, a graduate student of Prof. Rajakumara Eerappa from the Indian Institute of Technology (IIT) Hyderabad, India. I had a working experience of 3 years in the Centre for cellular and molecular biology (CCMB) under the guidance of Dr. Puran Singh Sijwali and worked as a research associate in the Institute of Genomics and Integrative Biology (IGIB) under the guidance of Dr. Archana Singh for one year. I was working as Project scientist II in the Translational Health Science and Technological Institute (THSTI) under the guidance of Dr Milan Surjit.
Research experience from [hidden] expertise (in research) belongs to the cloning and purification of proteins and their biophysical and computational approaches to investigate the molecular interactions of proteins to histone peptides. Most of my [hidden] research work focused on the UHRF1 (Ubiquitin-like, containing PHD and RING finger domain). We have demonstrated that the binding affinities of UHRF1 TTD exhibit higher binding affinity towardsH3K9me2 compared to H3K9me1/3through isothermal titration calorimetry (ITC). We have employed a computational tool, Molecular Dynamic (MD) simulation, to characterize the methyl status-specific binding of H3R2K9me by the TTD-PHD, and to analyze the effects of H3K4 di-methylation on H3R2K9me recognition. We have validated the MD simulation data through ITC binding study. These studies establish that the UHRF1 TTD-PHD exhibits lower binding free energy (ΔGbind) towards H3R2K9me2 compared to H3R2K9me3. We also showed that the SRA domain of UHRF1 binds to all 5mC oxidation derivatives.
Research Associate: In CCMB, I was working on biochemical and biophysical characterization of DNA Damage inducible (DDi1) protein from Plasmodium falciparum. Ddi1 is a retroviral aspartyl protease and is involved in the repair of DNA-protein crosslink. Ddi1 also acts as a proteasome shuttle factor. We investigated the Plasmodium DDI1 to identify its roles during parasite development and potential as a therapeutic target. We have also knocked in pfDDi 1 into Saccharomyces cerevisiae to investigate the growth effect.
In IGIB, I was working on isolation of Keratinocytes, Melanocytes and Fibroblast cells from foreskin patient samples and Cloning, Expression and purification of Transglutaminase 1(TGM1) protein from [hidden]
In THSTI, I was involved in the Development of a recombinant vaccine against Hepatitis E Virus and their immunological characterization.
Skills in Brief: I have also trained in molecular biology, biochemistry, and structural biology related techniques and also experienced in mammalian cell culture, Saccharomyces cerevisiae and Pichia pastoris. I am very well experienced in protein biochemistry, biochemical and biophysical characterization of proteins and epigenetics related concepts and experiments.
Research Interest: I am interested in DNA- protein, RNA- Protein and peptide-protein interactions, protein biochemistry, Structural biology and Epigenetics. My main goal is to setup independent research lab in good academic institute.
I believe that based on my research experience and skill set gained, I would perform to the best of ability for the given project. I am a highly motivated, innovative individual who works well independently as well as collaboratively on teams of scientists from various backgrounds. I also had experience in mentoring and training both undergraduate and postgraduate students with little prior research skills.
Thank you.
Sincerely,
Dr. M. Angel Nivya.