Introduction:
Dr. Khushbu Islam is a dedicated Junior Research Fellow and Teaching Assistant at the School of Life Sciences, Jawaharlal Nehru University, specializing in molecular biology, genetics, and biotechnology. She holds a [hidden] in Life Sciences and has a proven track record of academic achievements and research contributions.
Education:
- [hidden] in Life Sciences, Aligarh Muslim University, Aligarh, India, 2017-2023. GPA: [hidden]
- Master of Science in Biotechnology, Aligarh Muslim University, Aligarh, India, 2015-2017
- Bachelor of Science (Honours) in Botany, Zoology, and Chemistry, Aligarh Muslim University, Aligarh, India, 2012-2015. Percentage: [hidden]
Work Experience:
Dr. Khushbu Islam’s research skills and responsibilities include:
- Designing and executing experiments
- Data analysis and manuscript preparation
- Training and mentoring students
- Laboratory supervision and practical class teaching
Achievements:
- Qualified National Eligibility Test for Junior and Senior Research Fellowship from CSIR-UGC (2016)
- Qualified Graduate Aptitude Test in Engineering (2017)
Publications:
- Published research articles in reputable journals
- Authored review articles in the field of molecular biology and biotechnology
Workshops and Conferences:
- Received the Best Poster Award at a National Conference on Climate Change Impact
- Participated in various international and national conferences related to biotechnology and life sciences
References:
- Dr. Nirala Ramchiary – [hidden]
- Dr. Vandana Jaiswal – [hidden]
Skills:
R2R3-MYB transcription factors, Molecular markers, Transcriptome sequencing, Plant tissue culture, Genetic engineering, Biophysical techniques, Software Proficiency: R, TASSEL, ImageJ, Graphpad Prism, and more.
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Cover Letter:
Dear Hiring Manager,
I, Dr. Khushbu Islam, [hidden] from Jawaharlal Nehru University, New Delhi, am writing to express my enthusiastic interest in the Research Data Analyst position at IIT BHU. I am highly motivated for this role as it aligns with my experiences and skills in research data analysis and representation for publishing.
My doctoral research focused on investigating the role of MYB transcription factor genes in capsaicinoids (pungency) biosynthesis in _Capsicum_ species. This involved a multifaceted approach, including:
– Genome-wide identification of _MYB_ genes in _Capsicum _through comparative genomics and bioinformatic tools.
– Gene expression profiling of _MYB_ genes in three fruit developmental stages of low and high pungent _Capsicum_ species_ _using transcriptome sequencing, differential gene expression analysis, co-expression analysis and qRT-PCR.
– Isolation of _MYB_ genes from high pungent _Capsicum chinense_ for cloning and overexpression, in low pungent _Capsicum annuum_ via Agrobacterium-mediated plant transformation, tissue culture (shoot and root regeneration), transgenic development and screening.
– GFP tagging of MYB transcription factor proteins, agroinfiltration in _Capsicum_ leaf epidermis and subcellular nuclear localisation using confocal microscopy.
– Interspecific crossing of contrasting pungency containing chilli pepper species (_Capsicum annuum _and_ Capsicum chinense_) and development of F2 population
– Phenotyping- Extraction and estimation of capsaicinoids- capsaicin and dihydrocapsaicin in a large chilli pepper germplasm and F2 population using HPLC.
– Genotyping- Identification, development and validation of molecular markers (SSRs, InDels and SNPs) in MYB gene and promoter sequences for marker-assisted selection of high pungency trait in large germplasm of chilli pepper varieties and F2 population.
– Association analysis between phenotypic data and marker allele data using TASSEL.
Beyond my doctoral work, I have actively engaged in collaborative research projects, such as:
– Isolation of pungency biosynthesis pathway genes- _Amt, Kas_ and _Pun_ genes from highly pungent _Capsicum chinense_ and transgene expression in cherry tomato using GATEWAY cloning.
– Whole proteome analysis of fruit developmental stages of high and low pungent _Capsicum_ species using LCMS-MS to identify differently abundant proteins.
– Biolistics transformation of onion peel for protein-protein interaction studies of MYB transcription factors with pungency biosynthesis pathway proteins- Pun, Amt and Kas using BiFC.
– Metabolite analysis of eggplant under nematode _(Meloidogyne incognita) _stress and alleviation (biocontrol _Ganoderma lucidium_ macrofungi) conditions using GCMS.
– Metabolite analyses of _Capsicum_- roots, leaves, stem, and fruits under mineral (Phosphate, Iron and Zinc) element starvation and abundance stress via GCMS.
– Extraction and estimation of terpenoids (Phytosterols) in _Premna herbaceae _leaves and roots_ _via GCMS.
– Extraction and estimation of carotenoid and anthocyanins pigments in pepper fruits using HPLC.
My recent postdoctoral research focused on integrating proteomics, transcriptomics and metabolomics to identify differentially abundant transcription factors involved in capsaicinoids biosynthesis in _Capsicum chinense_. This involved several methodologies such as:
– Whole metabolite extraction from two fruit developmental stages of high and low pungent _Capsicum_ species and estimation and analysis via GCMS.
– Correlation analysis between proteome, transcriptome and metabolome data to understand the complex capsaicinoid biosynthesis pathway in _Capsicum chinense_.
Further to advance in my professional career, I am eager to join the Research Data Analyst position at IIT BHU, as it aligns with my research data analysis experiences. I am highly open to learning opportunities and excited to upgrade to latest data analysis softwares and tools. I aim to earn an edge to my research career with this challenging role.
I have attached my CV with list of publications and welcome the opportunity to further discuss my qualifications in an interview.
Looking forward to hearing from you soon.
Sincerely,
Dr. Khushbu Islam
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