Prayasi Gangopadhyay

Address: Kalapet, Pondicherry

Education

• Master of Science, Bioinformatics – 2023-2025

University: Pondicherry University

Status: Final year student

CGPA: 9.66

• Bachelor of Science, Zoology – 2021-2023

University: Calcutta University

Pass subjects: Botany, Chemistry

CGPA: 8.499

• 12th Boards – 2020

Board: Central Board of Secondary Education

Score: 89%

• 10th Boards – 2018

Board: Indian Certificate of Secondary Education

Score: 92%

Positions

• Representative of the Class – 2024

Institution: Pondicherry University

Performed CR duties and managed the day to day running of class for the first year

• Co-editor of College Magazine – 2023

Institution: Serampore College

Curated the entries and designed layout for the Des vu magazine circulated in college

• Teaching Intern – 2021-2022

Organization: RSSI NGO, Lucknow

Mathematics and biology teacher to underprivileged children from 10th standard

Scientific Work

• Research Intern – 2024

Institution: National Institute of Biomedical Genomics

Analysis of human DNA damage repair genes involved with head and squamous cell carcinoma

• Project Intern – 2024

Institution: Institute of Bioinformatics

Study of marine fungal genomes for discovery of novel biosynthetic gene clusters using computational tools

Languages

• Human: English, Bengali, Hindi
• Computer: C/C++, SQL, Java, Perl, Python

Workshops and Seminars

• Workshop on SBDD using Schrodinger Suite – 2024

Organization: Dept of Bioinformatics, Pondicherry University

Participated in this cloud based hands-on workshop for molecular modelling and docking simulations

Achievements

• Research Internship Awards – 2024-25

Organization: IIT Bombay, Dept of Biosciences & Bioengineering

Selected to work on a project on antibody development using AI

Skills: Bioinformatics, Molecular Modelling, Data Analysis, Research, Teaching, Computational Biology, Genetics, Scientific Writing

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Cover Letter:

To whomsoever it may concern,

I’m Prayasi Gangopadhyay, a final year masters student of Bioinformatics at Pondicherry University. I am at the top of my class and extremely interested in the field of data analysis in genomics. My dissertation project is in Structural Bioinformatics under Dr. Murali, where I predict the in 3d structure of a bacterial multifunctional enzyme in silico. But, a brief winter internship last December at NIBMG, under Dr NK Biswas exposed me to the world of oncogenomics and I have been hooked ever since.
Moving forward, I want to enhance my skills in computational genomics and contribute to the best of my abilities. I believe my limited experience in the field is compensated by my sincere efforts and knack for learning new things. Therefore, I would like to join the Medgenome team immediately after my course completes.
Looking forward to a positive response from your side

Prayasi Gangopadhyay
Murali Lab
Pondicherry University

“`

Cover Letter:

Respected Sir/ Ma’am
I am excited to apply for the Genome Analyst position at MEDGENOME. With my MSc in Biotechnology and experience in molecular biology and genomics, I am confident in my ability to analyze genetic data and contribute to research. My skills in bioinformatics and data interpretation align well with your requirements for this role. I would appreciate the opportunity to discuss how my expertise can benefit your team. Looking forward to your response.

Sincerely,
Subhashree Sahu

Introduction:

Deepthi Kallanja is a skilled professional in the field of biotechnology and computational biology. With a strong educational background and hands-on work experience, Deepthi has developed expertise in programming, bioinformatics, data analysis, machine learning, cloud computing, and software development. She has a proven track record of contributing to research projects, internships, and certifications, showcasing her proficiency in the application of advanced tools and techniques in biological data analysis.

Education:

• B.E. Biotechnology – Thadomal Shahani Engineering College • GPA: 8.59
• MTech Computational Biology – Anna University • GPA: 9.63

Skills:

• Python, BASH, R, SQL
• Cytoscape, BLAST, Galaxy, GATK, BWA, ANNOVAR, BCFTOOLS, Trimmomatic, Bowtie2
• Pandas, NumPy, Scikit-learn, ggplot2, Shiny, Bioconductor
• Data visualization, statistical analysis, data mining
• AWS (EC2, S3), Docker, Git
• Project Management, Biological Databases (NCBI, Ensembl, UCSC Genome Browser, PDB, TCGA)

Work Experience:

• Beaniesnak Foods Pvt. Ltd • Mumbai • 06/2019-07/2019
  R & D Intern
  • Development and Recipe testing for nutritional drink
  • Preparation of nutritional profile for the drink
• Haystack Analytics Pvt Ltd • Mumbai • 11/2022-04/2023
  Bioinformatics Intern
  • Genome sequencing projects
  • NGS analysis pipeline development
  • Data Visualization and Analysis for yeast genome

Projects:

1. Analysing mutations and protein-protein interactions in Sars cov-2
   • Analyzed mutations in SARS CoV-2 proteins
   • Created protein-protein interaction networks using Cytoscape
   • Performed functional analysis to identify key proteins
2. A comparative study of biosurfactant extracted from bacillus subtilis and lactobacillus plantarum
   • Determined properties of biosurfactants
3. Reference based RNA-Seq Data analysis using Galaxy
   • Identified differentially expressed genes and pathways in Drosophila cells
   • Analyzed RNA-Seq data
   • Pinpointed specific genes affected by the depletion of Pasilla genes

Certifications:

• Data Analysis with R Programming – COURSERA with Google | Jan 2024
• Big Data, Medicine and Genes: Machine Learning for Classification and analysis of Biological Data – COURSERA and John Hopkins | April 2024
• Databases and SQL for Data Science with Python – COURSERA and IBM | Sept 2024

Publications:

• SARS-CoV-2: analysis of the effects of mutations in non-structural proteins. Published in Archives of Virology, Springer • 06/2023
• Medicinally important plants: A Review Published in INTERNATIONAL JOURNAL FOR RESEARCH & DEVELOPMENT IN TECHNOLOGY • 06/2020

Skills:

Programming, Bioinformatics Tools, Data Analysis, Machine Learning, Cloud Computing, Software Development, Biological Databases

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Cover Letter:

I’m contacting you about the bioinformatician post.
My educational background includes a Bachelor’s Degree in Biotechnology Engineering from Mumbai University and a Master’s in Computational Biology from Anna University, Chennai.
During my academic journey, I have gained proficiency in Linux and have become familiar with NGS techniques and terminology. I am comfortable with Python and eager to develop my skills further. My experience includes assisting in NGS data analysis, supporting bioinformatics pipeline development for variant calling and data visualization, and collaborating with scientists to interpret genomic data. Additionally, I have maintained and troubleshooted Linux-based systems, engaging in problem-solving and workflow optimization. I have dabbled in Machine Learning Programming and know the various algorithms used for classification and prediction such as Decision Tree, Bayes, and SVM.

During my internship at Haystack Analytics, I gained hands-on experience in NGS analysis, Linux, bash scripting, and pipeline development using tools like BWA, ANNOVAR, BCFtools, GATK, and SAMtools. I developed a reference-based NGS automated pipeline for mapping, variant calling, and detecting resistance-mediating and phylogenetic variants from Illumina whole genome sequence data of Mycobacterium tuberculosis complex isolates from patient sputum-derived DNA samples. I have obtained certifications in Machine Learning for Biological Data from (Johns Hopkins University), Data Analysis in R (Google), and SQL using Python (IBM).

My educational background and the skills I have gained so far will be of utmost help professionally and I am always eager to learn more.

FIROZ TYAGI

Professional Summary

Firoz Tyagi is a Biotechnology graduate with a strong foundation in NGS, Bioinformatics, and Genomic Data Analysis. Holding a Master’s in Biotechnology with hands-on experience in DNA/RNA extraction, PCR, sequencing techniques, and molecular docking. Passionate about genomic research, variant analysis, and precision medicine, actively seeking an entry-level opportunity as a Genome Analyst to apply bioinformatics expertise and grow in the field of genomics.

Technical Skills

• Wet Lab Techniques
  • DNA and RNA extraction, PCR amplification, Gel electrophoresis, Cloning techniques
• Bioinformatics & Computational Biology
  • Sequence analysis, Next-Generation Sequencing (NGS), Molecular Docking, MD Simulation, Genomic Variant Analysis
  • Database management: NCBI, UniProt, Ensembl, PDB, KEGG
  • Software & Tools: BLAST, ClustalOmega, AutoDock, Schrödinger, PyMOL, GROMACS, GATK, Bioconductor
• Protein Purification & Analysis
  • Chromatography techniques (Basic understanding of HPLC, TLC, Affinity Chromatography)
  • SDS-PAGE, Western Blotting, Enzyme assays
• Research Methodology
  • Experimental design, Data interpretation and analysis, Literature review

Computer Skills

• Python
• Microsoft Office Suite (Word, PowerPoint, Outlook)
• Collaboration Tools
• MS Excel
• Troubleshooting

Interpersonal Skills

• Communication
• Teamwork
• Problem Solving
• Critical Thinking
• Time Management

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This HTML code can be directly published on a WordPress post.

Cover Letter:

15,Choudhary House 3rd Floor
Okhla Jamia Nagar New Delhi,110025
11/02/2025

Hiring Manager
MedGenome
Electronic City Bangalore 560100

Subject: Application for Junior Genome Analyst Position

Dear Hiring Manager,

I am eager to apply for the Junior Genome Analyst position at MedGenome. With a Biotechnology (BSc & MSc) degree from DTU and expertise in NGS, genome sequencing, and bioinformatics, I am excited to contribute to your research.

I have completed a Basic to Intermediate NGS Certification from Biotecnika, and my bioinformatics dissertation focused on computational disease research. My project work mainly revolved around bioinformatics-driven drug discovery, which deepened my interest in this field. During my internship at Jamia Millia Islamia, I worked on protein purification and drug discovery using plant-based phytochemicals.

As a fresher, I am eager to apply my knowledge and skills to support MedGenome’s focus on genomics-driven healthcare. I look forward to the opportunity to contribute to your team.

Thank you for your time and consideration.

Sincerely,
Firoz Tyagi

Karri Anusha

Location: Bengaluru

Profile

A Bioscience Post Graduate with a strong foundation in Biosciences and specialized expertise in Bioinformatics,
including hands-on experience in CADD and NGS.

Education

• 2019/06 – 2021/04
  B.Ed
  Sri Sathya Sai Institute of Higher Learning
  Action Research on effect of rote learning on children’s cognitive abilities.
• 2017/06 – 2019/05
  MSc in Biosciences
  Sri Sathya Sai Institute of Higher Learning
  Masters dissertation on Isolation, screening, and characterisation of cellulolytic bacteria from soil.
• 2014/06 – 2017/05
  B.Sc in Biosciences
  Sri Sathya Sai Institute of Higher Learning

Skills

• Molecular Biology Techniques
  CRISPR Cas9, ELISA, SDS PAGE, Agarose gel electrophoresis, plasmid isolation, DNA isolation, RNA isolation, protein isolation, PCR, cell transformation, preparation of competent bacterial cells.
• Microbiology
  Primary and secondary screening of bacterial isolates, cell-line maintenance, culture preparation, equipment handling.
• Immunological Techniques
  Dot ELISA, RH grouping, Double immuno-diffusion, Blood grouping, etc.
• BioInformatics
  NGS, Molecular Biology techniques, Drug discovery using CADD pipeline, bioinformatics databases.
• Plant Tissue Culture Techniques
  Preparation of plant culture media, cell and callus cultures, aseptic conditions.
• Biochemistry Techniques
  Protein estimation, fatty acids estimation, cholesterol estimation, Enzyme kinetics, etc.

Courses

• 2024/08 – 2024/10
  Advance Bioinformatics Certification course, Biogrademy
• 2024/08 – 2024/10
  Generative AI, GUVI geek networks, IITM research park
• 2024/02 – 2024/03
  Molecular Biology Techniques, Biotechnika

Professional Experience

• 2024/06 – present
  Intern at Biotechnika
  Working on a project titled “Identifying plant-based ligands against 3Clpro of SARS-CoV-2 virus”
• 2024/01 – 2024/06
  Franklin International Research Excellence Scholar at Biotechnika
  Completed internship in Molecular Biology techniques and Bioinformatics.
• 2022/05 – 2022/07
  Junior Content Writer at Straive
  Writer and curator of alt text scripts for Pearson Publications.
• 2020/06 – 2021/12
  Vidya Vahini PYF Mentor (voluntary) at Sri Sathya Sai Vidya Vahini
  Offered online English tutoring to teachers.
• 2020/05 – 2021/08
  Tutor (Self Employed)
  Provided online tutoring for Biology, Physics, Chemistry, and English to students.

Keywords: Molecular Biology, Microbiology, Immunological Techniques, Bioinformatics, Plant Tissue Culture, Biochemistry Techniques

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Cover Letter:

Dear Hiring Manager,

I am writing to express my interest in the Genome Analyst position at Medgenome. As a Bioscience Post graduate student , I developed a strong foundation in molecular biology, genomics, and bioinformatics, and I am eager to apply my skills in a practical setting.

During my Bioinformatics apprenticeship, I gained hands-on experience in Next-Generation Sequencing (NGS), whole genome DNA sequencing, and molecular biology techniques. I also became proficient in Linux environments and shell scripting, which allowed me to work efficiently with large datasets and automate data analysis processes. Additionally, I have gained familiarity with key bioinformatics databases such as NCBI, PUBMED, PDB, OMIM, and Ensembl, which have been invaluable in conducting research and gathering genetic information.

I am particularly interested in the intersection of genomics and drug discovery. Through coursework and projects, I have explored the CADD pipeline and its role in identifying potential therapeutic compounds. I am excited to contribute to drug discovery, NGS and genomic research in a dynamic team environment.

I am enthusiastic about the opportunity to bring my skills and passion for Bioinformatics to Medgenome, and I am eager to contribute to your innovative research efforts. Thank you for considering my application. I look forward to discussing how I can contribute to your team.

Sincerely,
Anusha

PRAVEEN KUMAR N

ABOUT ME

440/1, Earikadu, Mangalapuram (Po)
Rasipuram (TK), Namakkal (Dt) – 636 202
Praveen Kumar Natarajan

WORK EXPERIENCE

JUNIOR RESEARCH FELLOW
Dec 2022 – Nov 2024
CENTRE FOR OCEAN RESEARCH | SATHYABAMA UNIVERSITY

PUBLICATIONS

• Simulated marine heatwaves in mesocosms, integrating multi-omics data for photo physiological insights.
• Used bioinformatics tools for gene expression profiling and epigenetic analyses of seagrass under thermal stress.
• Integrated metabolomics data to identify metabolic pathways and adaptive responses to climate change.
• Applied transcriptomic data for gene expression analysis.
• Danaraj, Jeyapragash, Ushani Uthirakrishnan, Subasankari Kumaresan, Praveen Kumar Natarajan, Jayachandran Krishna, Ayyappasamy Sudalaiyadum Perumal, K. Selvakumar, Zohreh Karami, and Arivalagan Pugazhendhi. “Multifunctional application of seagrass-derived rosmarinic acid in mitigating biofilm and quorum-sensing virulence transcripts of methicillin-resistant Staphylococcus aureus.” Journal of Environmental Chemical Engineering (2024): 113086. (Impact Factor: 7.7)

CONTACT

2) Dr. S. GOWRISHANKAR
Assistant Professor
Department of Biotechnology, Science Campus,
Alagappa University, Karaikudi 630 003 Tamil Nadu, India

REFERENCES

• Dr. D. Jeyapragash
  Assistant Professor/Scientist- B (Research)
  Centre for Ocean Research, Sathyabama Research Park,
  Sathyabama Institute of Science and Technology, Chennai 600 119, Tamil Nadu, India

EDUCATION

B.Sc. Biotechnology – 85%
Muthayammal College of Arts and Science, Rasipuram

• HSC – 89.75%
  KPM Hi Tech Matric Hr. Secschool
• SSLC – 96.8%
  KPM Hi Tech Matric Hr. Secschool
• Diploma in Medical Laboratory Technology – 79.5%
  Muthayammal College of Arts and Science, Rasipuram
• M.Sc. Biotechnology – CGPA – 8
  DISSERTATION “Cloning and overexpression of Pseudomonas phage PSPa endolysin” carried out under the guidance of Dr. S. Gowrishankar at The Department of Biotechnology, Alagappa University in Karaikudi from December 2021-June2022 (DST-Funded Project) Alagappa University, Karaikudi

ACHIEVEMENTS

• Presented a paper by poster entitled, “Seagrass Ecosystem as a Blue Carbon Reservoir in Climate Change Mitigation” at DST-SERB sponsored national Conference on Integrative Omics in the Ocean Climate Change on 10th February 2023 organized by Centre for Ocean Research, Sathyabama University – 2nd Place.
• Presented a paper by poster entitled, “Reverse Vaccinology: Advancement Driven by Genomics against Bacterial and Viral Pathogens” at International Virtual Conference on COVID 19; Myths and Facts on 14th July 2021 organized by Mohanlal Sukhadia University awarded 2nd Place.

SKILLS

Whole genome sequencing data annotation and functional analysis (NGS), Gene expression analysis, Bioinformatic analysis, Epigenetic analysis, Phylogenetic analysis, Molecular cloning & Primer Designing, PCR techniques, High throughput drug screening, Animal Model handling (Galleria mellonella, Zebra Fish & Mice), Virus Cultivation, Programming language: Python, Other software/Tools: Editing-Canva, Biorender, and Microsoft Office.

“`

Cover Letter:

Respected Hiring Manager,
 I am Praveen Kumar N who worked as Junior Research Fellow at Sathyabama Institute of Science and Technology, Chennai for 2 years. I’m writing to apply for the position of Genome analyst position in MEDGENOME company, Bangalore.
My experience includes: NGS analysis. Whole genome sequencing, RT-qPCR, differential gene expression analysis and Bioinformatic analysis. Metabolite isolation and GC-MS sample preparation, GC-MS analysis and data interpretation. My technical skills include proficiency in tools like BWA and BLAST for plant genome analysis and clinical samples. Additionally, I am well-versed in statistical analysis and pathway enrichment tools such as KEGG.I investigate seagrass stress biology, focusing on epigenetics, metabolomics, and transcriptomics.
During my M.Sc. dissertation, I worked on recombinant protein expression and therapeutic enzyme engineering using the_ E. coli_ expression system and also evaluated anti-quorum sensing properties against clinical Pseudomonas strains using Galleria mellonella as an animal model.
My experience with plant and bacterial specific genomic analysis, coupled with my skills in variant detection, gene expression studies, and epigenomics, aligns seamlessly with the technical and biological expertise you are seeking. Moreover, my ability to stay abreast of emerging technologies and research trends ensures that I can contribute innovative solutions to your projects.
I am confident that my qualifications and enthusiasm for contributing to the field of genomics will make me an asset to MEDGENOME. I would welcome the opportunity to discuss how my skills and experiences can support your mission.

Thank you for considering my application. I have attached my resume for your review and look forward to the opportunity to further discuss my suitability for this role.
Sincerely,
**Praveen Kumar N.**

**G Shubhada Rao**
24, Umesh Layout, Bettahalasuru, Bengaluru, 562157, India

**Professional Summary**
Motivated and detail-oriented M.Sc. Biotechnology graduate with a strong molecular biology, DNA extraction, and PCR foundation. Experienced in conducting laboratory experiments, analyzing sequencing data, and collaborating with interdisciplinary teams. Skilled in genetic research, mammalian cell culture, and molecular diagnostics. Passionate about contributing to advancements in genomic sciences and seeking to apply expertise as a Junior Genome Analyst at MedGenome.

**Skills**
**Technical Skills:**
– DNA/RNA Extraction & PCR Techniques
– Gel Electrophoresis & Molecular Diagnostics
– Literature Research & Basic Bioinformatics Data Interpretation
– Microsoft Office Suite for Data Reporting

**Soft Skills:**
– Strong Analytical & Critical Thinking
– Effective Communication & Teamwork
– Attention to Detail in Research & Data Handling
– Ability to Work Under Pressure

**Education**
M.Sc. Biotechnology | REVA University, Bengaluru (06/2022 – 08/2024)
CGPA: 7.88

B.Sc. Biotechnology | REVA University, Bengaluru (04/2019 – 05/2022)
CGPA: 8.58

Pre-University Course (PUC) | Nagarjuna Pre-University College, Bengaluru (05/2017 – 03/2019)
Percentage: 88.16%

SSLC | Sri Krishna English Medium High School, Anantapuram, AP (03/2017)
GPA: 9.8

**Internship Experience**
Intern | ICAR-NIVEDI (01/2024 – 04/2024)
– Performed DNA extraction, PCR amplification, and gel electrophoresis for detecting Brucella melitensis in sheep samples.
– Conducted molecular biology experiments related to pathogen detection.
– Assisted in analyzing genetic data and sequencing results for research applications.
– Collaborated with biotechnology researchers and lab teams for sample processing.
– Gained experience in scientific literature curation and research documentation.

**Certifications & Training**
– Nanopore Sequencing Technology Training | Genotypic & REVA University (06/06/2023 – 07/06/2023)
– Mammalian Cell Culture Technologies | Biozeen (09/10/2023 – 13/10/2023)

**Languages**
– English
– Hindi
– Kannada
– Telugu

**Hobbies & Interests**
– Music
– Painting
– Photography

**Skills Keywords**
DNA/RNA Extraction, PCR Techniques, Gel Electrophoresis, Molecular Diagnostics, Literature Research, Bioinformatics Data Interpretation, Microsoft Office, Analytical Thinking, Communication, Teamwork, Attention to Detail, Pressure Handling.

Cover Letter:

G Shubhada Rao
24, Umesh Layout, Bettahalasuru
Bengaluru, 562157, India

11-02-2025

Hiring Manager
MedGenome Labs Ltd.
Bangalore, India

Subject: Application for Junior Genome Analyst Position

Dear Hiring Manager,

I am writing to express my interest in the Junior Genome Analyst position at MedGenome. As a recent M.Sc. Biotechnology graduate with practical experience in molecular biology, genetics and microbiology, I am confident I can contribute meaningfully to your innovative genomic research and diagnostic projects. My internship at ICAR-NIVEDI provided valuable hands-on experience in DNA extraction, PCR amplification, and gel electrophoresis. This also honed my laboratory skills, particularly in sequencing data analysis, and fostered my ability to collaborate effectively within an interdisciplinary team. I am particularly drawn to MedGenome’s leading work in genome research and diagnostics. The opportunity to collaborate with bioinformatics and laboratory experts on variant analysis and research-driven projects strongly aligns with career aspirations. My analytical mindset, meticulous attention to detail, and proven ability to thrive in a team environment make me a strong candidate for this role.

Thank you for your time and consideration. I have attached my resume for your review and welcome the opportunity to discuss my qualifications further.

Best regards,
G Shubhada Rao

DR. D. DINESH

Objective: Recent Ph.D. graduate in Zoology with a strong background in Liver Cancer, Mosquitoes, and Microbiology. Eager to utilize academic knowledge and research experience to drive meaningful advancements in the field.

Experience Performance:

College Name: Central Polytechnic College, Chennai

Designation: Visiting Lecturer / Fisheries Technology & Navigation Engineering

Duration: 09th Jan 2023 to Present

Education Performance:

Qualification	Year	Board of Examination	Name of The Institution	% of Marks
Ph.D. Zoology	2018-2022	Loyola College, Chennai, Affiliated to University of Madras	Loyola College, Chennai	Completed the Ph.D. degree
M.PHIL. Zoology	2016-2017	Loyola College, Chennai, Affiliated to University of Madras	Loyola College, Chennai	71%
B.ED Biological Sciences	2014-2015	Tamil Nadu Teachers Education University	C S JAIN Teacher Training Institute	60%

Awards:

• The best presentation awards at Prist University

Research Experience:

• Liver cancer and Mosquitoes
• Microbiology

Techniques Known:

• Ethnopharmacology
• Chromatography
• Extraction and Isolation of compounds from medicinal plants

Personal Profile:

Father’s Name: N. Dharmalingam

Date of Birth: 07.06.1993

Gender: Male

Nationality: Indian

Marital Status: Unmarried

Blood Group: O + ve

Hobbies: Browsing, Reading Articles and books, Research Work, Teaching, Listening to music, Writing poems

Sports: Cricket, Kabadi, Athletics, Football

Permanent Address: No: 5/151, South Street, Vayalur Village (post), Kunnam (Taluk), Perambalur (District), Tamil Nadu, India

Languages Known: English, Tamil

Declaration:

I hereby declare that the information furnished above is true to the best of my knowledge and belief.

Date:

Place:

(DR. D. DINESH)

Skills:

Zoology, Liver Cancer, Microbiology, Bioinformatics, Chromatography, PCR, Protein Purification, Microbial Techniques, Cellular Biology, Molecular Biology, Research Methodology

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Cover Letter:

Greetings sir/Madam. I am Dr. D. Dinesh. I have completed the Ph.D. Zoology department in Loyola College Chennai. I have two years experience in teaching job. I am interested to join the job. Kindly please help me sir/Madam. Thank you.

With regards
Dr. D. Dinesh

AMIT LADHA

Molecular Biotechnologist

“`

Cover Letter:

**Amit Ladha**
Kolkata, India
Date: 11/02/2025
**Hiring Manager**
MedGenome Labs Ltd.
Bangalore, India
**Subject:** Application for Genome Analyst Position
Dear Hiring Manager,
I am writing to express my keen interest in the **Genome Analyst** position at MedGenome. With a **Master’s in Molecular Biotechnology** from the **University of Birmingham** and hands-on experience in **Next-Generation Sequencing (NGS) technologies**, I am eager to contribute to your mission of advancing genomic diagnostics. My background in **variant analysis, molecular diagnostics, and bioinformatics collaboration** aligns well with the role’s requirements.
During my tenure as a **Research Lead at Rancelab Pvt Ltd**, I conducted **Whole Exome Sequencing (WES) on patient tumour samples** using **Illumina platforms**, ensuring data integrity through **FASTQC-based quality control**. I mapped sequences using **BWA** and performed variant calling with **GATK**, successfully identifying **driver mutations in oncogenes and tumour suppressor genes**. My expertise extends to **targeted gene panel sequencing**, where I analyzed **BRCA1/BRCA2 variants**, classified them using **ClinVar and dbSNP**, and assessed clinical relevance through **PubMed and HGMD literature curation**.
My ability to **collaborate across disciplines** is demonstrated by my work with **bioinformatics teams** in processing large genomic datasets. I performed **RNA-Seq analysis** using **STAR, HTSeq, and DESeq2** to identify differentially expressed genes, supporting precision medicine initiatives. Additionally, my experience in **designing molecular diagnostic assays**—including **multiplex PCR primers for CYP450 gene variants**—would be valuable for assay development at MedGenome.
Beyond technical expertise, I bring **strong analytical and communication skills**, allowing me to **interpret sequencing results, prepare clinical reports, and effectively collaborate with cross-functional teams**. My commitment to precision and efficiency is reflected in my role in optimizing **biotinylation efficiency in Turbo-ID constructs**, achieving **85% accuracy in experimental validation**.
MedGenome’s pioneering efforts in clinical genomics and large-scale projects such as **GenomeAsia 100K** resonate with my passion for cutting-edge genomic research. I am excited about the opportunity to apply my expertise in **NGS, human genetics, and molecular diagnostics** while growing within an innovative team. I look forward to discussing how my skills can contribute to MedGenome’s vision.
Thank you for your time and consideration. I have attached my **CV** for your review. I would welcome the opportunity for an interview to explore this further.
Sincerely,
**Amit Ladha**

Rohan Ramesh Thorat

Market Yard Supe, Baramati, Pune

Objective

Seeking a dynamic role to leverage my recent postgraduate education, aiming to continuously enhance my skills and deliver top-tier performance. Enthusiastic about contributing knowledge, skills, and abilities in an industry that fosters competitiveness and innovation, providing growth opportunities while remaining adaptable and inventive.

Experience

Fresher

Education

• Proficient in chromatographical techniques such as HPLC, TLC, GC.
• Skilled in microbiological techniques including pouring, streaking, spreading, and inoculation.
• Experience in handling biotechnological instruments such as autoclaves, incubators, magnetic stirrers, laminar air flow systems, centrifuges, ultracentrifuges, and UV spectrophotometers.
• Handling experience in techniques like Antimicrobial susceptibility test, Disc diffusion, Well diffusion, Micro broth dilution, PCR, and SDS PAGE.

Projects

• Development of Pullulan with Nanocomposite for antimicrobial Coating System.
• Successfully isolated Aureobasidium sp. from bathroom walls during project work. Isolates were characterized by 18S rRNA sequencing, identifying the isolated fungi as Aureobasidium melanogenum.

Interests

• Research and Development.
• Manufacturing.
• Quality Control and Quality Assurance.

Languages

• Hindi
• English
• Marathi

Workshop Attended

• Fermentation Technology
• Mastering Biofertilizers for Entrepreneurial Success
• One-day Hands-on Training ‘Pharmaceutical Techniques and Career Navigation with Interview Skills’
• Statistical Software used in Biostatistics

Hobbies

• Playing Chess
• Reading
• Traveling

Skills

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Cover Letter:

My name is Rohan Ramesh Thorat, and I recently completed my postgraduate degree in Microbiology from Tuljaram Chaturchand College (Savitribai Phule Pune University) Baramati. I am proud to have maintained an excellent academic record, and I am confident that this foundation will translate into a strong professional performance.

I have attached my resume for your review and kindly request that you consider my application for the available position within your team.

Thank you for considering my candidacy. I look forward to the opportunity to contribute to your esteemed organization.