ALEENA JAMES CHIRAYIMMEL
GENOME ANALYST
Molecular Biology professional with expertise in gene expression, NGS analysis, and bioinformatics. Skilled in RT-PCR, genetic variant interpretation, and uncovering key regulatory genes in biological pathways. Passionate about advancing genomic research through precise molecular techniques and data-driven insights.
WORK EXPERIENCE
Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala
Research Trainee – Dr. Moinak Banerjee (Human Molecular Genetics Lab) | Aug 2023 – Jan 2024
- Successfully conducted and optimized RT-PCR assays for precise gene expression analysis, leading to the identification of key regulatory genes in various biological pathways, protein interactions, and cellular pathways.
- Designed and executed gradient PCR and standard PCR experiments, resulting in enhanced amplification efficiency and specificity for target genes.
- Developed and validated custom primers for multiple genes, significantly improving the reliability and reproducibility of molecular biology experiments.
- Optimized experimental protocols and methodologies to enhance scientific understanding and research efficiency.
Eminent Biosciences, Indore, Madhya Pradesh
Bioinformatics Analyst | Jan 2022 – May 2023
- Efficiently queried and extracted genomic, proteomic, and pathway information from biological databases such as NCBI, Ensembl, UCSC Genome Browser, and UniProt, leading to the identification of key molecular targets.
- Expertly utilized molecular visualization tools like PyMOL and RasMol to interpret complex molecular structures and visualize protein-ligand interactions, resulting in high-impact publication-quality images.
- Proficiently employed Marvin Sketch to create and edit chemical structures, generating accurate 2D and 3D molecular representations and calculating molecular properties, which facilitated the design and analysis of novel compounds.
- Conducted comprehensive bioinformatics analysis of NGS data, including alignment and variant calling using BWA and SAM tools, leading to the discovery and interpretation of significant genetic variants for advanced genomic research.
EDUCATION
MSc Biotechnology | Vellore Institute of Technology, Vellore, Tamilnadu | Graduation Date: May 2022
BSc Biotechnology | St Joseph College Irinalakuda, Thrissur, Kerala | Graduation Date: May 2020
PROJECT EXPERIENCE
Master Thesis – Eminent Biosciences, Indore, Madhya Pradesh | Jan 2022 – Jun 2023
Computer-aided drug design for the identification of high-affinity BACE1 inhibitors for the clinical treatment of Alzheimer’s disease
CERTIFICATIONS
- R Programming: Coursera – John Hopkins University
- Drug Discovery – UC San Diego
- AI – ML Internship – Biotechnika (30 days)
PUBLICATIONS
- Medicinal Plants/Herbs in Respiratory Syncytial Virus and Viral Pneumonia, [hidden]
- Nanomaterials as Drug Delivery Vehicles for Therapy of Parkinson’s Disease, [hidden]
- Nanomaterials for Diagnosis and Treatment of Common Neurological Disorders, DOI: [hidden]
- Nanocarriers and Nano Drug Delivery Therapy in Neuro Diseases, DOI: [hidden]
SKILLS
Laboratory Techniques: RT-PCR, Gel electrophoresis, Bacterial DNA isolation, Isolation of DNA from blood, Nanodrop, cDNA synthesis, RNA Isolation, Gradient PCR, Normal PCR, Sequencing PCR, Cell Culture Techniques
Soft Skills: Scientific Research & Publications, Team Collaboration, Data Interpretation, Survey Design
Bioinformatics tools: BLAST, Clustal Omega, MEGA, SOPMA
NGS Tools: FASTQC, Bowtie2, TopHat, HISAT2, Samtools, Cufflinks, StringTie
“`
Cover Letter:
Respected Hiring Manager,
I am writing to express my interest in the Genome Analyst position at MedGenome. With a Master’s degree in Biotechnology from VIT and hands-on experience in molecular biology and bioinformatics, I am eager to contribute my expertise to your genomics research and diagnostics team.
My academic and research background has equipped me with a strong foundation in next-generation sequencing (NGS) data analysis, variant annotation, and computational biology. I have experience using bioinformatics tools such as BWA, SAMtools, and Ensembl, along with molecular visualization software like PyMOL and RasMol. Additionally, my practical knowledge includes PCR techniques, Sanger sequencing, and primer designing, which align well with MedGenome’s focus on genomic data interpretation and personalized medicine.
What excites me most about this opportunity is MedGenome’s commitment to advancing precision medicine through cutting-edge genomic research. I am particularly interested in how genomics can be leveraged to understand neurological diseases and personalized medicine, aligning with my passion for translational research. Given my experience in data extraction from biological databases (NCBI, UCSC Genome Browser, UniProt) and my ability to analyze complex genomic datasets, I am confident in my ability to contribute meaningfully to your team.
I would welcome the opportunity to discuss how my skills and experience align with the role. Please find my resume attached for your review. Thank you for your time and consideration, and I look forward to the possibility of joining MedGenome.
Yours Faithfully,
Aleena James Chirayimmel