Akshayee Pradeep Mahajan

Address: 402, Nishchint, Tulsibaugwale Colony, Sahakarnagar, Pune 411009, India

Education

Master of Science, Medical Biotechnology
University of Illinois at Chicago, College of Medicine, Rockford
2016 – 2018, IL, USA

Bachelor of Technology, Biotechnology
University of Pune, Pune, India
2011 – 2015

Work Experience

ClientCurve Pvt. Ltd, India – Business Development Consultant – Biotechnology & Pharmaceuticals

September 2023 – August 2024
– B2B Lead Generation – Cold Emailing & Calling Reach outs to USA-based Biotechnology & Pharmaceutical Companies

Genomebio Technologies Pvt. Ltd, Pune, India – Research Intern

September 2022 – February 2023
– Molecular biology techniques-gel electrophoresis, PCR, Nucleic acid extractions, qPCR, measuring DNA concentrations by Qubit, Spectrophotometer etc.

Evolvus Technologies Pvt. Ltd, Pune, India – Biocurator/Scientific Data

2019 – 2022
– Scientific data analysis/curation of research articles for post translational modifications (PTMs) of cell signaling molecules (enzymes, transcriptional co- activators, repressor, different receptors etc)

University of Chicago, Chicago, IL – Research Assistant

February – May 2019
– Laboratory of Dr. Luis Barreiro, Genetics Section – Culturing and maintaining iPSCs, Culturing and maintaining Lympho-blastoid cell line, RNA extraction, RT-qPCR etc

University of Pennsylvania, Philadelphia, PA, USA – Research Specialist

August 2018 – January 2019
– Laboratory of Dr. Kenji Murakami – DNA isolation from yeast, Small-scale purification of HIR- complex proteins from yeast using IgG beads (using TEV cleavage) followed by checking proteins by gel electrophoresis, Growing yeast cells using Fermenter and post fermenter harvesting, Cryo-grinding of yeast cells using Cryo-mill, TCA precipitation for concentrating protein fractions, gel shift assay, Elongation assay etc.

Whitehead Institute for Biomedical Research, MA, USA – Research Intern

June – December 2017
– Dr. Rudolf Jaenisch’s Laboratory – Master’s Thesis – Project title: Optimization of Epigenome-Editing Tools (modification of histone- acetylation targeting module)- Regulation of MyoD expression by modification of histone-acetylation targeting module/machinery (already prepared), using cloning in HEK293T cells.
Techniques performed: Western blot, RT-qPCR, ChIP- qPCR, Cloning, Cell culture, Transfection, Transformation, Plasmid/DNA extraction, RNA extraction, Cloning based on restriction enzymes, Agarose-gel electrophoresis, SDS-PAGE, Restriction digestion

geneOmbio Technologies Private Limited, Pune, India – Research Intern

February – June 2016
– Project Title: Development of PCR & Sequencing based method for detection of important Single Nucleotide Polymorphisms (SNPs) in CYP2D6 genes having pharmacogenetic significance. Extracted DNA from human blood samples, purification of PCR samples by ExoSAP-IT, Sample preparation for sequencing by Sanger’s dideoxy method (sequencing reaction in machine set up by experts), Agarose gel- electrophoresis, Designed a new pair of PCR primers for CYP2D6*3 and CYP2D6*4 and optimized PCR condition, basic bioinformatics tools etc.

National Chemical Laboratory (NCL), Pune, India – Research Assistant

June 2014 – May 2015
– Dr. Absar Ahmad’s Laboratory, Project Title: Biosynthesis of Highly Stable Silver Nanoparticles Using Guizotia Abssynica (Thistle) Seed Extract, Their Complete Characterization and Antimicrobial Studies. Prepared plant and mushroom samples for screening using UV-Vis Spectrometry. Selected samples were further characterized by Fourier Transform Infra-red Spectrometry (FTIR), Transmission Electron Microscopy (TEM), X-Ray Diffraction (XRD). Tested antibacterial and antifungal properties of powdered Guizotia Abssynica AgNP.

Co-Curricular Activities

• Poster presentation for titled “Identification of Drug Hypersensitive Human Topoisomerase 2 Mutants” for ‘Rockford Research Day’ at University of Illinois at Chicago, College of Medicine, Rockford, IL (April 2016)
• Delivered seminar on ‘Bioluminescence: Introduction Biosynthesis And Its Potential Applications’ as a part of curriculum to the class during 3rd year of B.Tech (March 2014)
• Attended ‘Frontiers in Chemistry & Biology of Oligosaccharides’ Seminar at IISER Pune, India (January 2014)
• Attended state level seminar on ‘Advances in Enzyme Technology’ sponsored by Board of Colleges and University Development, University of Pune (March 2013)
• Secured 3rd position in Model Presentation Competition in 2nd year of Bachelor’s, organized by ‘OPERON’, Department of Biotechnology, SCOE, Pune (March 2013)

Short Internships during Undergrad

• Acton Biotech Pvt. Ltd, Pune, India – June 2014
• Emcure Pharmaceuticals Pvt. Ltd, Pune, India – June 2013
• Serum Institute of India Pvt. Ltd, Pune, India – December 2012

Skills

Keywords: Biotechnology, Pharmaceuticals, Molecular Biology Techniques, Data Analysis, Research, Laboratory Techniques

Cover Letter:

I am Akshayee Mahajan from Pune. I have completed a Master of Science in Medical Biotechnology and am currently exploring career opportunities in laboratory work and Biotech/Pharma Business Development.

        Monika P R
        
        SUMMARY:
        To be a successful professional in the field of Biochemistry by utilizing my skills and growing consistently along with the organization.
        
        CONTACT DETAILS:
        #44b, 1st main 6th cross Lakshmi devi nagar, Bangalore-560096
        DOB: 03 August, 1998
        
        EDUCATION:
        
        2019-2021 MSc BIOCHEMISTRY 
        JSS COLLEGE OF ARTS, COMMERCE AND SCIENCE (AUTONOMOUS), OOTY ROAD, MYSURU-25
        CGPA: 8.95
        
        2016-2019 BSc- CHEMISTRY, ZOOLOGY AND BIOTECHNOLOGY 
        MES DEGREE COLLEGE, BENGALURU-03
        CGPA – 8.38
        
        2014-2016 12TH STANDARD - PCMB
        KLE INDEPENDENT PU COLLEGE, BENGALURU-72
        81.33%
        
        2014 10TH STANDARD
        OXFORD ENGLISH SCHOOL
        81.12%
        
        WORK EXPERIENCE:
        
        • Worked as Junior QA officer at Biogen Extracts Pvt. Ltd for a duration of 7 months, Dobaspet Bangalore
        • Worked as a project associate in the Department of Fruit and Vegetable Technology of CSIR-CFTRI for a duration of one year
        
        CERTIFICATIONS:
        
        • 2023 Presented poster on “DEVELOPMENT OF NUTRITIONALLY ENRICHED PASTA FORTIFIED WITH SEAWEED ULVA LACTUCA AND SHELF-LIFE ASSESSMENT” 
        • 2021 INTERNATIONAL WEBINAR ON ‘ACCELERATING RESEARCH- THE WAY FORWARD’ BY KARYOME
        • 2021 CERTIFICATE OF PARTICIPATION, TWO DAYS INTERNATIONAL E-CONFERENCE ON DRUG DISCOVERY AND MATERIAL SCIENCE
        
        ACHIEVEMENTS:
        
        • 2019 THIRD PRIZE IN ENGLISH ESSAY COMPETITION ON “INTEGRITY- A WAY OF LIFE” ORGANIZED BY POST GRADUATE DEPARTMENT OF COMMERCE IN ASSOCIATION WITH NEW INDIA ASSURANCE COMPANY LIMITED.
        
        RESEARCH AND ANALYTICAL SKILLS:
        
        ➢ Food analysis proximate composition (estimation of moisture, fat, dietary fibre, sugars, ash- with mineral estimation)
        ➢ Sensory analysis and food microbiology (total plate count)
        ➢ Phytochemical and antioxidant analysis – (total phenolics, total flavonoids, total antioxidant activity, DPPH, and ABTS)
        ➢ Texture and color instrument handling and interpretation
        ➢ Experience in animal handling, hematology, serum biochemistry, histopathology analysis and interpretation
        ➢ Skilled in analytical techniques: RP HPLC, UV-visible spectrophotometer, Rotary evaporator, gel permeation chromatography, TLC, gel electrophoresis, SDS page, Lyophilizer, Fluorescence spectrophotometer.
        
        STRENGTHS:
        
        • Fast learner
        • Time management
        • Organizational skills
        • Technical skills
        • Inquisitive and ability to comprehend
        • Tutoring
        • Problem-solving
        • Flexibility
        
        ADD ON COURSES:
        
        • Diploma in Bioinformatics from Maharani Lakshmi Ammani College, Bengaluru
        • Diploma in Biostatistics from MES Degree College, Bengaluru
        • Completed research project on Recombinant DNA Technology from Shridhar Bhat’s Laboratory
        
        INTERESTS:
        
        • Tutoring
        • Reading
        • Chess
        
        LANGUAGES:
        English, Telugu, Kannada, Hindi
    

SKILLS:
Biochemistry, Analytical Skills, Research Skills, Food Analysis, Protein Analysis, Spectrophotometry, Chromatography, Laboratory Techniques, Data Analysis, Microsoft Office

“`

Cover Letter:

I am enthusiastic and determinant of the field of my work and able to work both in groups and individually.

PRIYANKA BHOSALE
MICROBIOLOGIST

G.P.R.A. Quarters Type II near Akurdi Railway Station, Pune

PROFILE

Microbiologist with competent laboratory skills, who is enthusiastic and highly adaptable. Passionate about new scientific findings and their impact on the field. Keeps up-to-date on the latest developments in Microbiology through extensive reading of Research Papers. Meticulous with a minute attention to details and good observation skills.

LANGUAGES

• English
• Hindi

EDUCATION

• Fergusson College Pune (2023-2025)
  Masters in Microbiology
  MSc – 7.5 CGPA
• D.Y Patil College Akurdi Pune (2020-2023)
  Bachelors in Microbiology
  9.0 CGPA
• D.Y Patil College Akurdi Pune (2019)
  Higher Secondary Education, Pune
  68.9%

AREA OF INTEREST

• Clinical and Medical Microbiology
• Virology
• Immunology
• Advance Molecular Biology

SKILLS

• Microbiological Techniques: Sterilization, culturing techniques, isolation, identification
• Instrumentation: UV-visible Spectrophotometer, pH meter, centrifuge, UV transilluminator, Gel Electrophoresis

CERTIFICATIONS

• Swayam online course on Animal Biotechnology Course (71%) by University of Calicut
• Basics of Nutrition (ICMR) online course
• Communication Skills online course by Sabira Merchant’s (SPPU)
• Typing certificate GCCTBC (English 40wpm, Hindi and Marathi 30wpm)
• MSCIT (79%)
• Indian classical Harmonium player (Praveshika Purna)

INTERNSHIP

• Jankalyan Raktapedhi Pune
  – Conducted blood grouping, infection screening (Malarial Plasmodium antigen test)
• Operated lab equipment (Centrifuge, weighing blood bags) in component separation lab
• Assisted in Blood Collection, storage
• Assisted blood donors and guided them

DISSERTATION

• ICMR-National Institute of Virology Pune
  Topic: Isolation and Characterization of Varicella Zoster Virus from referred clinical specimens on MRC-5 cell strain (BSL-2 lab)
• Tissue culture, Virus handling, DNA PCR

SKILLS

• Microbiological Techniques
• Instrumentation
• UV-visible Spectrophotometer
• pH meter
• Centrifuge
• UV transilluminator
• Gel Electrophoresis

“`

Cover Letter:

Priyanka Bhosale
Akurdi, Pune
Maharashtra
3 June 2025
Dear Hiring Manager,
I am writing to express my keen interest in the microbiologist position, as advertised. As a recent graduate in Microbiology, I am eager to begin my career in a challenging and growth-oriented environment where I can apply my academic knowledge, laboratory training, and passion for scientific research.
During my undergraduate studies, I gained hands-on experience in microbiological techniques including culturing, staining, aseptic techniques, and microbial identification, tissue culture, PCR. My final year project focused on Isolation and characterization of Varicella Zoster Virus from the referred clinical specimens on MRC-5 cell strain which strengthened my skills in experimental design, and scientific communication.
In addition to my technical skills, I bring strong attention to detail, the ability to work collaboratively in lab teams, and a commitment to maintaining high standards in safety and accuracy.
I would welcome the opportunity to further discuss how my background and enthusiasm align with your team’s goals. Thank you for considering my application. I have attached my resume for your review and would be grateful for the opportunity to speak with you at your convenience.

Sincerely,
Priyanka Bhosale

SHIVAM KRISHNAM JENA

CAREER OBJECTIVE

To attain a challenging role within a visionary organization where I can fully harness my potential, deepen my expertise, and contribute significantly to the organization’s success and growth.

ACADEMICS

• Master of Science (Pharm.) in Biotechnology
• Board: NIPER S.A.S. Nagar
• Institute: National Institute of Pharmaceutical Education and Research (NIPER), S.A.S. Nagar
• Passing Year: 2025
• Percentage/C.G.P.A: 7.82 in the Third semester
• Bachelor of Pharmacy
• Board: Biju Patnaik University of Technology, Rourkela, Odisha
• Institute: College of Pharmaceutical Sciences, Puri, Odisha
• Passing Year: 2023
• Percentage/C.G.P.A: 8.95
• H.S.C.
• Board: Central Board of Secondary Education
• Institute: Pragati Public Senior Secondary School, Baran Road, Kota, Rajasthan
• Passing Year: 2017
• Percentage: 86.60%
• S.S.C.
• Board: The Council for the Indian School Certificate Examination
• Institute: St. James’ Convent School, Jaleswar
• Passing Year: 2015
• Percentage: 93.33%

RESEARCH AND WORK EXPERIENCE

• Research Project: “Optimization of process parameters for the growth of E.coli expressing recombinant human paraoxonase I (variant I) by Response Surface Methodology” (2024-2025)
• Research: Pharmacognosy, phytochemical screening, the antioxidant activity of Sauropus androgynus (2022-2023)
• Training: Hands-on training program at Biotecnika in Global Regulatory Affairs
• Medical Training: One month at C.H.C Jaleswar during the 4th year of B.Pharm
• Certification: Completed Good Clinical Practice (GCP) online training

COURSES AND CERTIFICATIONS

• Regulatory Affairs, Regulatory Authorities, Clinical Trial processes, CTD and eCTD filing, ICH GCP Guidelines, Development Lifecycles of drugs and medical devices, IND, NDA, ANDA, MAA Filing.
• Labelling, IFU development, Risk Management Plan for Medical Devices, Post Marketing Surveillance.

PERSONAL DETAILS

• Address: Jaleswar, Baleshwar, Odisha

AREAS OF INTEREST

• Global Regulatory Affairs
• Drug Development Process
• Clinical Trials
• Market Research
• Quality Compliance
• ICH guidelines
• Pharmacovigilance

KEY STRENGTHS

• Analytical Skills
• Attention to Details
• Communication Skills
• Planning and Execution
• Adaptability

SKILLS

• MS Excel
• MS Word
• MS PowerPoint
• Odia (Language)
• English (Language)
• Hindi (Language)
• Learning Spanish

ACCOMPLISHMENTS

• Achieved AIR 796 in GPAT 2023
• Achieved AOR 11 in OJEE 2023
• Achieved AIR 241 in NIPER JEE 2023

“`

Cover Letter:

Respected Sir / Ma’am
I am Shivam Krishnam Jena from NIPER, Mohali. I am enthusiastic and looking for opportunity for documenting process in Quality assurance and control. Currently, I am pursuing a hands-on training program which helped me to understand basic concepts about it.
Your kind consideration in this regard will be highly solicited.
Regards
Shivam Krishnam Jena

Kumari Priyatama

Address: Moharda Near Vijay Garden Baridhih, Jamshedpur

SUMMARY:

Passionate and dedicated biotechnology enthusiast with a strong foundation in molecular biology, genetic engineering, and bioinformatics. Actively seeking a position to apply my skills in advancing research and innovation, aiming to contribute to significant scientific discoveries and breakthroughs in the field.

EDUCATION:

2022 – 2024
M. Sc Biotechnology, Siksha O Anusandhan University, Bhubaneswar
Percentage secured: 80.75
Relevant Coursework: Molecular Biology, Genetic Engineering, Plant and Animal Biotechnology, Microbiology, Bioinformatics, Cell Biology.

2018 – 2021
B. Sc Zoology, Ramadevi Women’s University, Bhubaneswar
Percentage secured: 79.00
Relevant Coursework: Molecular Biology, Microbiology and Cell Biology, Plant ecology and Plant Biotechnology.

2016 – 2018
Higher Secondary (Science), AIWC Academy of Excellence, Jamshedpur
Percentage Secured: 69.00
Felicitated with certificate of excellence for securing first division in annual examination.

2015 – 2016
Secondary, AIWC Academy of Excellence, Jamshedpur
Percentage Secured: 74.00

RESEARCH EXPERIENCE:

Feb 2025: AI/ML & Computational Biology (from Biotecnika)

• Basic understanding of AI/ML applications in drug discovery and bioinformatics
• Hands-on exposure to data interpretation, predictive analysis, and biological modeling tools
• Familiar with tools like SwissADME, Protox, ChemSketch, and Chemspace

Jan 2024-Jun 2024: Project Work, Institute of Life Science Bhubaneswar

• Validation of blood biochemical and hematological parameters of mouse, rat, hamster

Sept 2023-Sept 2023: One day Workshop, ICMR-Regional Medical Research Centre, Bhubaneswar

• Molecule visualizing and validating structural models of protein targets, identifying biological ligand binding sites
• Hands-on Laboratory Experience in real-time lab experiments
• Use tools including SwissADME, ADMETSAR, Protox, Chemical sketch tool, Chemspace

Sept 2023-Sept 2023: One day Workshop, IIT Bhubaneswar

• Bioinformatics Software, Molecular Modeling and Visualization
• Use of softwares Autodock and Raccoon

CURRICULAR SKILLS:

Biological Skills

• Molecular Biology Techniques (PCR, Gel Electrophoresis, DNA/RNA Extraction)
• Genetic Engineering (CRISPR, Cloning)
• Biochemical Analysis (Spectrophotometry, Chromatography)
• Microbiological Techniques (Culturing, Staining)
• Bioinformatics Tools (BLAST, Gene Ontology)
• Data Analysis (Excel, R, Python)
• Laboratory Safety and Protocol Compliance
• Technical Writing and Documentation

Computational Skills

• Bioinformatics Tools (BLAST, MEGA)
• Molecular Modeling Software (PyMOL, Chimera)
• Writing Scripts for Data Analysis

Laboratory and Safety Skills

• Understanding of Biosafety Levels
• Proper Handling of Chemicals and Biological Samples
• Waste Disposal Procedures
• Proper Use and Maintenance of Lab Instruments
• Calibration and Troubleshooting of Equipment

Research Skills

• Designing Hypothesis-Driven Research
• Conducting Controlled Experiments
• Systematic Review of Scientific Literature
• Accurate Data Collection Methods
• Statistical Analysis Techniques

Complementary Skills

• Scientific writing
• Teaching
• Leadership
• Problem solving
• Willingness to learn
• Multi-tasking

IT Skills

• Systems administration
• MS-Word, MS-Excel, MS-PowerPoint
• C and C++ programming

EXTRACURRICULAR SKILLS:

Teamwork

• Initiated the idea of international conference MicCon22
• Engaged and contributed in academic and non-academic activities during M. Sc. in Siksha O Anusandhan University

Languages

• English (professional proficiency)
• Hindi (professional proficiency)
• Odia (native)

Other Skills

• Scientific writing
• Teaching
• Creativity
• Communication
• Problem solving
• Time management
• Positive attitude
• Willingness to learn
• Multi-tasking
• Resilience

Keywords: molecular biology, genetic engineering, bioinformatics, PCR, CRISPR, BLAST, C++, teamwork, leadership, problem solving, time management, scientific writing

“`

Cover Letter:

Kumari Priyatama
Vill Moharda, Near Vijaya Gardens,
Baridih, Jamshedpur – 831017

Date: 2nd June 2025

Subject: Application for the Position of QA Documentation – Fresher

Dear Hiring Manager,

I am writing to express my interest in the position of QA Documentation at Rivarp Medical Pvt Ltd, as advertised. I have recently completed my Master’s degree in Biotechnology from Siksha ‘O’ Anusandhan University, Bhubaneswar, and am eager to begin my professional journey in the pharmaceutical and medical sector, where I can apply my academic knowledge and passion for quality and regulatory standards.

During my academic career, I gained hands-on exposure to documentation practices, SOP preparation, and quality compliance through laboratory-based coursework and an internship in computational biology. My understanding of regulatory documentation, data accuracy, and quality control aligns well with the requirements of a QA Documentation role.

I am a quick learner, detail-oriented, and committed to maintaining high standards in documentation processes. I am confident that my educational background and dedication to quality assurance make me a suitable candidate for this opportunity.

Thank you for considering my application. I would welcome the opportunity to discuss how my skills and enthusiasm can contribute to your team.

Sincerely,
Kumari Priyatama

Summary

Swagata Das is a highly motivated biotechnology professional with a background in Botany and Molecular Biology. Seeking challenging opportunities in the Biotech industry to utilize knowledge and technical skills for driving organizational success.

Education

SSC (80%)
Marishda Bijoykrishna Jagrihi Banipith (WBBSE) – West Bengal
Jan 2014 – Jan 2015

HSC (72%)
Contai Hindu Girls’ School (WBCHSE) – West Bengal
Jan 2015 – Jan 2017

Bachelor of Science: Botany (8.14 CGPA)
Prabhat Kumar College (Vidyasagar University) – West Bengal
Apr 2017 – Apr 2020

Master of Science: Molecular Biology (9.13 CGPA)
Maulana Abul Kalam Azad University of Technology – West Bengal
Apr 2020 – Apr 2022

Work Experience

Biocon Biologics Limited
INTERN (IPQA dept.)
Apr 2023 – Sep 2023
Sep 2023 – Jan 2024
– Review Executed BMR, PDR, Checklist
– Basic knowledge of Deviation, CAPA and Change Control
– Approval of label, Lysis batch approval
– Support in Data Collection
– Adherence to GDP, GMP, and cGMP Guidelines
– Reviewing Line Clearance

… (additional work experience details as provided) …

Certificates

• Advance Industrial Microbiology Training Course from Helix Biogenesis (Noida)
• Introduction to Probability from edX (HarvardX)
• Human Physiology from Coursera (Duke University)

Skills

• Cell Culture
• Bioreactor Operation
• UV-Spectrophotometer
• … (full list of skills provided in the data) …

References

Vikram Amuluru
Biocon Biologics Limited / Assoc. Manager
Phone: [Phone Hidden]
Email: [Email Hidden]

Sandeep Mollidain
Biocon Biologics Limited / QA (Asst. Manager)
Phone: [Phone Hidden]
Email: [Email Hidden]

Declaration

I, Swagata Das, hereby solemnly declare that what is stated is true to the best of my knowledge.

Project

Repurpose of Drug for CML Cancer Protein hIMPDH-II: A Study by Computational Drug Discovery Method
1) Identification of CML Cancer Receptor hIMPDH
2) Binding study of CML Cancer drugs and their structural analogues with protein hIMPDH

“`

Cover Letter:

With due respect and humble submission, I am writing this letter to you with a view to enquire about the possibility of getting a position in Biocon Biologics. Myself Swagata Das currently living at Bangalore, Karnataka. I worked as a Quality Assurance (IPQA) Intern at Biocon Biologics Limited, Bangalore. I worked in Bio Scientific Research Laboratories Pvt ltd in Mumbai as a Junior Analyst in Bioanalytical department. QA internship helps me in good understanding with GDP, GLP, GMP, and ALCOA++. I have basic knowledge of CAPA and Deviation. I have completed also a course of Industrial Microbiology. I will try my best to enable me to meet your high expectations. I am attaching my resume for your kind consideration. I am eagerly waiting to hear back from you. Thanks & Regards Sincerely, Swagata Das

CHAITHRA CP
Internship

Chaithra Cp
Manjeri

SUMMARY
Biotechnology professional with experience in genetic analysis and data interpretation, expert in PCR and bioinformatics tools. Key achievements include attending a national conference on molecular biology that enhanced understanding of biotechnology applications, and completing a course in clinical research fundamentals covering study design and regulatory requirements.

EDUCATION
Pursuing Master’s Degree in General Biotechnology
University of Calicut
2023 – Present

Bachelor’s Degree in General Biotechnology
University of Calicut
2020 – 2023

Biology science
GHSS Karakunnu
2018 – 2020

SKILLS
PCR
Gel Electrophoresis
DNA/RNA Extraction
Genetic Analysis
Cloning Experiments
Cell Culture
Bioinformatics Tools
Tissue Culture
Spectrophotometry
Recombinant DNA Technology
Scientific Writing
Data Analysis

INTERESTS
I have a strong interest in genetic engineering, molecular biology and microbiology,
and I’m passionate about using biotechnology to drive innovations in healthcare

PROJECTS
MOLECULAR CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS BY MOLECULAR MARKER
12/2022 – 01/2023
Bangalore, India
It involves identifying genetic traits to understand its diversity, virulence, antibiotic resistance, and epidemiology. Techniques such as PCR, DNA sequencing, and molecular typing methods are commonly used.

KEY ACHIEVEMENTS
National Conference on millet
Participated in a national-level conference focused on the nutritional value, agricultural importance, and biotechnological advancements related to millet

Clinical research course
Completed training in clinical research methodologies.
Completed a certified training program on Intellectual Property Rights
Gaining foundational knowledge of patents, copyrights, trademarks, and the role of IPR in research.
Training in Molecular Techniques in Nucleic Acid Analysis
Gained hands-on experience in techniques such as PCR, gel electrophoresis, nucleic acid extraction, and quantification methods.

Keywords: PCR, Gel Electrophoresis, DNA/RNA Extraction, Genetic Analysis, Cloning Experiments, Cell Culture, Bioinformatics Tools, Tissue Culture, Spectrophotometry, Recombinant DNA Technology, Scientific Writing, Data Analysis
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Copy and paste this HTML code directly into a WordPress post to display the formatted CV as requested.

Cover Letter:

Chaithra cp
Puliyattakunnu House karakunnu, manjeri, Malappuram, Kerala 676123

Manager
Rivarp medical pvt Ltd

Dear sir/Madam
I am writing to express my interest in the QA documentation position at Rivarp medical pvt Ltd, as advised on biotechnica. With a strong background in biotechnology, microbiology, and hands-on experience in molecular biology techniques and data analysis, I am confident in my ability to contribute meaningfully to your team. I have currently completed my masters in general biotechnology.

My expertise includes:
– Proficiency in molecular techniques such as PCR, gel electrophoresis, and DNA/RNA extraction.
– Experience with bioinformatics tools.

I am particularly drawn to Rivarp medical pvt Ltd. Thank you for considering my application. I would welcome the opportunity to further discuss how I can contribute to your team in an interview. I am available to join immediately.

Sincerely
Chaithra cp

Prafulla Srivastava

Profile

Dynamic and passionate biotechnologist with a strong foundation in both wet and dry lab practices. Proficient in handling advanced laboratory instruments and techniques, including SDS-PAGE, chromatography, plant tissue culture, and in silico gene characterization. Actively participated in esteemed national and international conferences, showcasing research aptitude and networking with global experts. Completed comprehensive training programs in biotechnology, enhancing technical and analytical skills. A well-rounded individual with leadership experience in cultural events, demonstrating exceptional teamwork and organizational abilities. Driven to excel in innovative biotechnological research and contribute to groundbreaking advancements.

Professional Experience

2024/06 – 2025/05

Project on Machine learning approach to find novel anti diabetic drug from natural sources

Biotecnika Info Labs Pvt Ltd

Role: Research Associate/Project Lead

Responsibilities:

• Designed and implemented a data-driven workflow integrating machine learning models and bioinformatics tools for drug discovery.
• Curated and pre-processed datasets of natural compounds, including their physicochemical properties, ADME profiles, and bioactivity data.
• Extracted molecular descriptors such as molecular weight, LogP, and Ali Log S using cheminformatics tools like RDKit and SwissADME.
• Developed and validated predictive machine learning models (Random Forest, Decision Tree, Linear Regression) to evaluate compound efficacy and drug-likeness.
• Conducted statistical analysis and visualization of data using Python.

2022/06 – 2022/07

Lucknow, India

Trainee

Experiome Biotech Private Limited

Completed training in biotechnology, acquiring expertise in wet lab techniques such as SDS-PAGE, chromatography, plant tissue culture, microbiology lab culture, and agarose gel electrophoresis. Proficient in dry lab practices like in silico gene family characterization. Skilled in laboratory protocols, data analysis, and molecular biology research methodologies.

2024/08 – 2025/03

Trainee

Biogrademy

Learning basics of Python and Biopython libraries.

2022/02

Lucknow, India

Trainee

CytoGene Research & Development LLP

Training of basic bioinstrumentation tools like protein quantification assays, Agarose gel electrophoresis, and DNA extraction from various sources.

Student Coordinator

Apni Pathshala, SRMU

Part of CSR initiative of the University at Apni Pathshala runs for taking care of primary studies of the students of surrounding villages.

2025/01 – 2025/06

Project dissertation

National Centre for Cell Science

• Investigated the role of secretory PD-L1 (secPD-L1) in modulating T-cell exhaustion and apoptosis using Jurkat E6.1 cell line models.
• Designed and optimized Western blot, pull-down assays, and co-immunoprecipitation to analyze PD-L1/PD-1 and FBXO family protein interactions.
• Hands-on experience in protein concentration (cut-off 3kDa), SDS-PAGE, PVDF transfer, antibody probing, and chemiluminescence detection.
• Performed ELISPOT/activation assays and used Jurkat + co-culture systems to validate secretory PD-L1’s functional role.

Education

• 2023/07 – present
• Hisar, India
• M.Sc. Biotechnology
• Guru Jambheshwar University of Science and Technology

Highly motivated MSc Biotechnology graduate with hands-on experience in advanced laboratory techniques, including column chromatography, centrifugation, SDS-PAGE, PCR, UV spectroscopy, and agarose gel electrophoresis. Proficient in handling laboratory equipment with precision and adhering to strict safety protocols. Actively participated in multiple national and international conferences, showcasing research work and engaging with global scientific communities. Demonstrates strong analytical skills, a commitment to innovation, and a passion for contributing to cutting-edge biotechnological advancements.

• 2020/08 – 2023/06
• Barabanki, India
• B.Sc. (Hons) Biotechnology
• Shri Ramswaroop Memorial University

Skills

• PCR
• Chromatography
• Gel electrophoresis
• HPLC
• Protein Purification
• Cell Proliferation Assays (MTT, CCK-8)
• Machine learning
• Cell cultures
• Python
• Molecular Cloning
• Immunoprecipitation (IP)
• Scientific Writing

“`

Cover Letter:

To
The HR Department
Rivarp Medical Pvt. Ltd.
Nayandahalli, Bangalore

Subject: Application for the Position of QA Documentation Executive

Dear Hiring Manager,

I am writing to express my keen interest in the position of QA Documentation Executive at Rivarp Medical Pvt. Ltd., as advertised on Biotecnika. As a postgraduate in Biotechnology with practical research experience and a strong foundation in documentation, compliance, and laboratory protocols, I am confident in my ability to contribute effectively to your quality assurance team.

During my M.Sc. in Biotechnology at Guru Jambheshwar University of Science and Technology and my dissertation training at the National Centre for Cell Science (NCCS), I was involved in hands-on experimental work including SOP preparation, maintenance of research records, compliance with lab protocols, and regulatory documentation. My experience has taught me the importance of precision, traceability, and quality control in scientific work.

Highlights of my qualifications include:

Familiarity with ISO 13485, GMP practices, and regulatory documentation

Proficiency in MS Office tools (Word, Excel, Outlook) for maintaining QA trackers and reports

Strong communication, attention to detail, and a proactive learning attitude

Ability to manage and archive BMRs, protocols, and quality documents effectively

I am particularly drawn to Rivarp Medical’s work in high-quality medical devices and its alignment with global regulatory standards. The opportunity to grow in a QA-focused documentation role within such an innovative and quality-driven organization aligns perfectly with my career goals.

I am excited about the possibility of contributing to your team and would welcome the opportunity for an interview. Thank you for considering my application.

Warm regards,

Prafulla Srivastava

Introduction:

Srayashe Das is a dedicated and accomplished individual with a strong academic background in Bio Engineering. With hands-on experience in protein biochemistry research and clinical laboratory work, Srayashe possesses a skill set encompassing laboratory techniques, instrumentation, software proficiency, and exceptional leadership qualities demonstrated through various professional and extracurricular engagements.

Education:

• National Institute of Technology, Agartala
  CGPA: 7.44
  Degree: Bachelor of Technology – Bio Engineering
  Year: 2022-2026
• Bhavan’s Tripura Vidya Mandir
  Percentage: 80.6
  Qualification: AISSCE (XII – PCMB) – CBSE
  Year: 2021-2022
• Bhavan’s Tripura Vidya Mandir
  Percentage: 83.0
  Qualification: AISSE (X) – CBSE
  Year: 2019-2020

Research Experience:

• Indian Institute of Technology, Kharagpur
  Duration: Dec 2024 – Jan 2025
  Position: Research Intern – Protein Biochemistry
• Expressed and purified recombinant TIGIT protein using E. coli BL21(DE3) systems achieving 85% yield efficiency
• Optimized protein refolding protocols improving inclusion body solubilization by 40%
• Performed affinity and size-exclusion chromatography achieving 95% protein purity verified by HPLC
• Analyzed protein samples using SDS-PAGE and Western blotting with quantitative densitometry
• Agartala Government Medical College
  Duration: May 2024 – Jun 2024
  Position: Clinical Laboratory Intern
• Performed 500+ ELISA assays with 99.2% accuracy for biomarker quantification and disease panel analysis
• Operated automated clinical analyzers processing 150+ samples daily with 100% QC compliance
• Conducted serum biochemistry analysis including lipid panels and liver function tests with CV ¡2%
• Implemented RT-PCR protocols for pathogen detection achieving 98% sensitivity and 99% specificity

Research Projects:

• Inhibitory Receptor TIGIT: Expression and Purification Study
• Investigated recombinant expression of T cell immunoreceptor with Ig and ITIM domains (TIGIT)
• Developed protocols for inclusion body solubilization and protein refolding optimization
• Applied nickel-affinity chromatography for His-tagged protein purification
• Characterized protein purity and yield using polyacrylamide gel electrophoresis

Technical Skills:

• Laboratory Techniques: Protein Expression, Chromatography (Affinity, Size-Exclusion), SDS-PAGE, Western Blotting, ELISA, PCR, RT-PCR, Gel Electrophoresis, HPLC
• Instrumentation: Automated Clinical Analyzers, Autoanalyzer, Easy Lyte, Spectrophotometer, Centrifuge, Incubators, Biosafety Cabinets
• Software & Analysis: Python, C Programming, Tableau, Power BI, Microsoft Office Suite, Statistical Analysis, Data Visualization
• Laboratory Management: Aseptic Techniques, Good Laboratory Practices (GLP), Quality Control, Sample Processing, Documentation
• Languages: Bengali (Native), English (Professional), Hindi (Fluent)

Certifications & Achievements:

• Deloitte Data Analytics Virtual Experience Program Certificate (2024)
• Tata Group’s Data Visualization Certificate (2024)
• State Topper – International English Olympiad, 12th Rank North East India, SOF (2021)
• Board Topper in Literature – CBSE Board Examination (2020)
• School Topper – International Science Olympiad, Silverzone Foundation (2017)
• NCC A Certificate Holder – Leadership and Discipline Training (2016-2017)

Leadership & Professional Activities:

• Training and Placement Coordinator | Career Development Cell, NIT Agartala (2025 – 2026)
• President & Lead Designer | AAVEG – Student Organization (2024 – 2025)
• Executive Member | MOKSHA IX – Socio-Cultural Festival (2024 – 2025)
• Hospitality Lead | AAYAM 8.0 – Technical Festival (2023 – 2024)
• Inter-NIT Sports Representative | Chess, Cricket, Kho-Kho (2023 – 2024)

Skills:
Bioengineering, Protein Biochemistry, Laboratory Techniques, Chromatography, ELISA, PCR, Data Analysis, Leadership, Event Management, Communication

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Cover Letter:

Dear Hiring Manager,
I am writing to express my interest in the QA Documentation position at Rivarp Medical Pvt Ltd. As a final-year Bio Engineering student at NIT Agartala with hands-on experience in clinical laboratories and research, I am excited about the opportunity to contribute to your quality assurance team.
During my clinical internship at Agartala Government Medical College, I performed over 500 ELISA assays with 99.2% accuracy and maintained 100% QC compliance while operating automated analyzers. This experience taught me the critical importance of precise documentation and quality control in medical settings. I also developed technical protocols during my recent research internship at IIT Kharagpur, where I optimized protein purification processes and maintained detailed laboratory records.
What draws me to Rivarp Medical is your commitment to healthcare excellence. My background in bioengineering, combined with my experience in Good Laboratory Practices and statistical analysis, aligns well with the documentation and quality standards your company maintains. I’m proficient in Microsoft Office Suite, data visualization tools like Tableau and Power BI, and have a strong foundation in analytical thinking and process improvement.
My leadership roles, including Training and Placement Coordinator and President of student organizations, have strengthened my communication skills and attention to detail – qualities essential for effective QA documentation. I’m particularly interested in how quality documentation supports patient safety and regulatory compliance in the medical industry.
I would welcome the opportunity to discuss how my technical skills, laboratory experience, and passion for quality assurance can contribute to Rivarp Medical’s mission. Thank you for considering my application.
Sincerely,
Srayashe Das

SUMMARY:

Name: SEETHALAKSHMI S

Education: M.Sc. Biotechnology (3.39 out of 5) & B.Sc. Zoology (7.86 out of 10) from Mahatma Gandhi University

Experience: Project Intern at Saveetha Institute of Medical and Technical Sciences

Skills: Laboratory Skills, DNA/RNA Isolation, PCR, Cell Culture, Enzyme Assays, Protein Extraction, SDS-PAGE, Microbial Techniques, Biogenic Nanoparticle Synthesis, etc.

Awards: Participated in various workshops, conferences, and seminars related to Biotechnology

EDUCATION:

M.Sc. Biotechnology (Mahatma Gandhi University) – 3.39 out of 5

B.Sc. Zoology (Mahatma Gandhi University) – 7.86 out of 10

EXPERIENCE:

Project Intern
Saveetha Institute of Medical and Technical Sciences
Investigated the eco-friendly biosynthesis of bimetallic Ag-Zn nanoparticles using Pseudomonas putida

SKILLS:

Laboratory Skills, DNA/RNA Isolation, Cell Culture, Enzyme Assays, Protein Extraction, SDS-PAGE, Microbial Techniques, Biogenic Nanoparticle Synthesis, Gram Staining, Bioinformatics Tools, etc.

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Cover Letter:

**Dear Hiring Manager,**
I am writing to express my interest in the QA Documentation position at your organization. With a strong academic background in Biotechnology and hands-on experience in maintaining accurate records, SOPs, and regulatory documentation, I am confident in my ability to contribute effectively to your Quality Assurance team.
I am highly detail-oriented, organized, and committed to compliance with industry standards. I would welcome the opportunity to support your documentation processes and uphold quality systems within your organization.
Thank you for considering my application. I look forward to the opportunity to discuss how my skills can benefit your team.
Sincerely,
SEETHALAKSHMI S