CHANDRA KUMAR MALLAH

Objective

Seeking a life science position to bridge academic knowledge with practical experience, aiming to gain valuable insights in research methodologies and their application in the industrial sector.

Experience

Project Intern at Centre for Cellular and Molecular Platform (C-CAMP), Bangalore. (Sept 2024 – Dec 2024)

Education

MASTER OF SCIENCE in BIOTECHNOLOGY (2022-2024) – REVA University, Bangalore. CGPA – [hidden]

BACHELOR OF SCIENCE in BIOTECHNOLOGY, GENETICS AND BIOCHEMISTRY (2018-2021) – Bangalore North University, Kolar. CGPA – [hidden]

HIGHER SECONDARY EDUCATION (2015-2017) – Presidency Pre-University College, Bangalore. Percentage – 83%

SECONDARY EDUCATION (2015) – St Peter’s school Panchgani, Maharashtra. Percentage – 73%

Project Work

“Study of production of Cellulase enzyme by Alternaria brassicicola through Solid State Fermentation in Ragi, Horsegram and Paddy straw as substrates” for the partial fulfillment of Master of Science Biotechnology.

Project guide: Dr Santharam KS

Key Skills

Microscopy, Microbiology, Molecular biology techniques, Genetic engineering techniques, Biochemical analytical techniques, Enzymology techniques, Bioprocess technology, Bioinformatics, Communication, MS Office

Certificates

Several hands-on training programs and NPTEL courses in the field of biotechnology.

Conferences

Participated in the 2nd international conference on “Global trends in applied sciences, medical and health sciences”

Activities and Interests

Chess, Reading, Wanderlust

References

1. Prof. Shilpa Rao – HOD & Director-in-charge, Dept of Biotechnology, REVA University, Bangalore

2. Dr. Santharam KS – Associate Professor, Dept of Biotechnology, REVA University, Bangalore

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Cover Letter:

I am writing to express my keen interest in joining your lab Molpath Pvt Ltd. My particular interest lies in the field of Microbiology, Molecular biology, Recombinant DNA technology and Animal Biotechnology. I am an open-minded person, looking to explore Life Science research beyond classroom academics, with my passion for biotechnology and curiosity in exploring biological life, I am eager to contribute my expertise and dedication to your team. During my academic and professional journey in biotechnology, I have cultivated a strong foundation in molecular biology, microbiology, and biochemistry—key disciplines for life sciences research. My project work was related to fungal biotechnology, which helped build basic Microbiology, Molecular biology and Biochemistry skillset. I have completed multiple skill development programs in Molecular biology and Microbiology, these experiences have honed my skills in laboratory techniques and critical data interpretation. I am excited to explore modern molecular biology and protein biochemistry/biophysics as I am looking forward to building a career in this field. One’s environment is always the key factor for career growth. Therefore, I am looking for opportunities at your lab. I am confident that my skills and passion align seamlessly with your mission, and I am eager to bring my problem-solving abilities and enthusiasm to your team. I would welcome the opportunity to discuss how my expertise and experiences align with your needs and how I can contribute to your efforts. I look forward to the possibility of contributing impactful work at your lab. Thank you.

Aastha Garg – Biotechnologist & Data Analytics Professional

Experience

• Research Trainee | INMAS-DRDO, Delhi (Jan ’24 – Present)
  Responsibilities: Designing 5HT7, CXCR4, and Sigma Receptor
  specific agents for neuroimaging. In-silico, synthesis, Invitro and In-vivo studies.
• Research Intern | Hazra LAB, IIT Roorkee (Feb ’23 – Present)
  Responsibilities: Multi-level In-Silico Analysis of Beta-Lactamases using various bioinformatics tools. Working with BKC, GPC, and BlaS enzymes.
• Research Intern | SCIS-JNU, Delhi (June ’23 – Sep ’23)
  Responsibilities: Analysis of Candida albicans using In-silico tools.
• Research Trainee | Genomics and Proteomics Lab, Amity, Noida (Nov ’22 – Jan ’23)
  Responsibilities: Research paper on etiopathogenesis profiling of Inflammasomes for Inflammatory Bowel associated Arthritis for biosensing applications.
• In-House Training | Amity University, Noida Campus (July ’22 – Aug ’22)
  Responsibilities: Worked with Dr. Shivani Sharda to identify Co-regulatory target in IBD correlated arthritis.

Skillset

Keywords: Bioinformatics, Structural Biology Tools, Multiple Sequence Alignment, Homology modeling, PyMol, Wincoot, Virtual screening, Molecular Docking, Molecular dynamic stimulation, MM/PBSA, MM/GBSA, Rstudio, Cytoscape, String, Gene Mania, KEGG, R-Programming, PCR, EL
ISA, Column Chromatography, Electrophoresis Techniques, MTT Assay, UV Spectrophotometer, Rotavapor, Cell Culture Techniques, Autoclave, Peptide synthesis.

Education

Bachelor of Technology in Biotechnology Amity University Noida (2024) – [hidden] CGPA

XII (CBSE) Delhi Public School Ghaziabad International (2018) – [hidden]

X (CBSE) Delhi Public School Ghaziabad International (2016) – [hidden] CGPA

Certificates and Achievements

• Thermo Fisher Scientific Mass Spectrometry Job Simulation on Forage (May ’24)
• Thermo Fisher Scientific Genetic Sciences Job Simulation on Forage (May ’24)
• Linux Fundamentals (LearnQuest) (Feb ’23)
• NLP with Deep Learning and Machine Learning (Intellipaat) (Jan ’23)
• Data Analysis with R Programming (Google) (Jan ’23)
• Industrial Biotechnology (University of Manchester) (Jan ’23)
• Data Analysis Professional Certificate (Google) (Oct ’22)

Position of Responsibility

• Member | Zehen, Music Society, AIB (Oct ’20 – Jan ’22)
• Volunteering – Scriptwriting | E-Cell, Entrepreneurship Club, AIB (Sep ’21)

Projects

Dissertation

Other Projects

Summer Internships

Winter Internships

In-house training

Publications

May ’24

Conferences and Workshops

• Attended BIC-NNP workshop on “Big Data Analysis and Machine Learning”
• Attended International Conference on Innovative Practices in Technology and Management, 2024
• Attended Skill Enhancement workshop on multi-omics data integration and data analytics
• Attended International e-Workshop on “Computational Approaches in Drug Design & Therapeutics”, 2021

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Cover Letter:

As a passionate supporter for advancing healthcare through innovative research and technology, I am thrilled to present my application for working as JRS at Molpath Pvt Ltd. During my undergraduate years, I actively engaged in research projects at different institutions that allowed me to apply theoretical knowledge to practical applications. Through internships and laboratory experiences in _School of Computational And Integrative Sciences_, **JNU**; _Radiological Nuclear Allied Imaging Sciences_, **INMAS-DRDO**; and _Hazra Lab_, **IIT Roorkee**, I gained hands- on experience in techniques such as peptide synthesis, cell culturing, animal handling and computational drug designing. These experiences not only reinforced my passion for research but also equipped me with essential laboratory skills and analytical thinking required for advanced scientific inquiry. I am confident that working at Molpath Pvt Ltd will provide me with the ideal platform to further explore these research interests under the guidance of esteemed faculty members and within a vibrant industrial community. I am confident that my academic background, research experiences, and passion for innovation make me a suitable candidate for this job. I look forward to the opportunity to contribute to the research endeavors of the department and to embark on a transformative journey at Molpath Pvt Ltd.

CV: Ashwini Patil

Summary

I am a self-motivated, Capable, and Hard-working employee wishing to pursue a challenging career in a progressive environment that demands the best of my skills and knowledge while contributing to the organization’s success and growth.

Working Experience

• Technical discussion with the client against the Inquiry for a sample test
• Proposal preparation against the Inquiry to onboard the client
• Prepare and maintain the Excel report to manage the data against the Inquiry

• Implement and Maintain standard Policies and guidelines at the Molecular Testing Laboratory
• Calibration of Molecular Laboratory Instruments and Maintaining the Calibration Documents
• Daily Instrument Maintenance and Quality Control
• NABL Documentation

• Skilled in Standard Laboratory Practices including Sample collection, Sample analysis, and Result uploading on the portal
• Interpretation of data and troubleshooting
• Procurement of kits (RT-PCR & RNA Extraction kit), Consumables for Molecular Lab & Vendor Management
• Preparing and managing all the documents related to Proprietary items, Laboratory consumables, and Asset items for the Internal Audit & Representing and participating in the External Audit conducted by ICMR- Headquarters

• Worked as Associate (LYTX- shipping and logistics) for International BPO at WNS Global Services

Technical Skills

• Molecular Diagnostic Laboratory
• Automated RNA purification system
• EQAS Sample testing
• ILC sample testing
• RT-PCR machine
• ELISA Reader
• Sanger Sequencing
• HLA typing
• Chromatography Techniques
• SDS Gel Electrophoresis Unit
• Blotting Techniques
• Chemical Oxygen Demand (COD) and Biological Oxygen Demand (BOD) analysis
• Microbiological Laboratory Techniques
• Instrumentation
• Lab-scale Reactor

Project Work

• The aim of this study is to Develop of food waste leachate treatment method with advanced biological techniques at the lab scale
• SBBGR reactor consists of both Aerobic and Anaerobic Condition
• Physical, Chemical, and Biological parameters testing with a fix interval of time
• SBBGR shows effective reduction of COD up to 93%

Educational Qualifications

Master of Science (Biotechnology) (2016-2018) – RTM Nagpur University, Nagpur, Maharashtra

Bachelor of Science (Life Science) (2013-2016) – RTM Nagpur University, Nagpur Maharashtra

Higher Secondary School (2013) – Maharashtra State Board, Nagpur

Secondary High School (2011) – Maharashtra State Board

Symposium Attended

• Organize the Workshop on the “Laboratory Diagnosis of SARS CoV2 by Real Time PCR” at ICMR-NICPR, Noida
• Attended a National Conference by Dr. Rajendra Singh (Water man of India and Nobel Prize winner) on Wet Land Biodiversity Conservation for sustainable future development at Dr. Ambedkar College, Nagpur
• Poster presented in National seminar on “Nano particles synthesized by bacterial cell in biomedical application” held at Kamala Nehru College, Nagpur, Maharashtra
• Participated in Hands on training programme “Isolation of pure culture”
• Participated in wetland & Biodiversity aptitude test by Maharashtra Forest Department Gov of MH2017

Personal Information

Date of Birth: 7th April, 1996

Permanent Resident Address: Plot No. 17 Warhadkar Layout, Hajari-Pahad, Nagpur, Maharashtra-440007

Marital Status: Single

Nationality: Indian

Declaration

I hereby declare that the information furnished above is true to the best of my knowledge.

“`

Cover Letter:

I’m sorry, but you haven’t provided any data for me to redact. Could you please provide the information that needs redacting?

**Curriculum Vitae**
**Summary**
**Biotechnologist** with extensive expertise in diverse areas of biotechnology, including reproductive biology, proteomics, transcriptomics, animal studies, and molecular biology. Adept at designing and conducting experiments, as well as analyzing and interpreting complex biological data. Specialized in investigating and manipulating reproductive processes at the molecular level.

**Education**
– **[hidden]** in Biotechnology (2012-2017), University of Kerala, Thiruvananthapuram.
– **Master of Science** ([hidden]) in Biotechnology, St. Berchmans College, Changanacherry, [hidden] University, Kottayam.
– **Bachelor of Science** ([hidden]) in Botany and Biotechnology, Govt. Arts College, Thiruvananthapuram.

**Research Experience**
– **CSIR Research Associate** (CSIR-RA)
– **Senior Research Fellow** (SRF) in CSIR-funded project
– **Senior Research Fellow** in a SERB-funded project

**Specific Skills**
– LC-MS and MALDI-TOF analysis
– Protein isolation and purification
– DNA sequencing and bioinformatics tools

**Awards and Memberships**
– Fellowship of CSIR Research Associateship (RA)
– Best Poster Awards for Presentation

**Publications**
– “DNA Methylation and Histone Modifications Associated with Male Germ Cell Differentiation”
– “Organization of Planar Rafts, Caveolae, and Steroid Receptors on Spermatozoa during Development”

**Conference Presentations**
– “Proteomics of Sperm Membrane Rafts During Epididymal Maturation and Capacitation”

**Administrative and Management Experience**
– Organized the International Conference of Repromics
– Active involvement in cultural programs

**Referees**
1. Dr. G. Pradeep Kumar
2. Dr. Vipin Gopinath
3. Dr. Malini Laloraya

Keywords: Biotechnology, Proteomics, Transcriptomics, Molecular Biology, Reproductive Biology

**DECLARATION**
I hereby declare that all the details furnished above are true to the best of my knowledge and belief.

**Place:** Thiruvananthapuram
**Date:** 18-07-2024
**Dr. SOUMYA A.**

Cover Letter:

Dear Hiring Manager,
I am writing to apply for the Junior Research Scientist position at Molpath Pvt. Ltd. As a highly motivated researcher with a [hidden] in Biotechnology and extensive experience in molecular biology, I am confident that my skills and expertise would allow me to make a significant contribution to your team.
I hold a [hidden] in Biotechnology from the University of Kerala (awarded in 2019). My doctoral research, entitled _”Molecular Evaluation of Interactions between Sperm Membrane Rafts and Zona Pellucida Proteins,”_ was conducted at the Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, under the guidance of Dr. G. Pradeep Kumar (Former Scientist, Sperm Repromics Lab) in the Molecular Reproduction Unit. Following my [hidden], I served as a CSIR-Research Associate (CSIR-RA) at RGCB, the title of my research grant was _”Identification and Characterization of a Functional Promoter of TDP-43”_. My research career started in June 2010 as a junior research fellow (JRF) in a KSCSTE-funded project progressing through roles in DBT, SERB, and CSIR-funded research projects.
During my 11-year tenure in research, I have honed a comprehensive suite of technical skills, including:
– Molecular biology techniques such as PCR, cloning, protein expression and purification, SDS-PAGE, and Western blotting.
– Mammalian cell culture, including protein overexpression and silencing.
– Advanced analyses such as immunocytochemistry, FACS, ultracentrifugation, and mass spectrometry (LC-MS/MS, MALDI-TOF).
– Collection of spermatozoa from different maturation stages, and _in-vitro_ capacitation and acrosome reaction.
– Retrieval of oocytes and embryos from different stages.
– Bioinformatics tools like BLAST, CLUSTALW, SMART analysis, STRING analysis, Panther analysis, and various biological databases for data interpretation and research insights.
Although my primary research area was reproductive biology, these versatile skills are applicable across diverse domains in biotechnology.
After taking a career break from February 2021 to May 2024 to prioritize family responsibilities, I recently resumed my professional journey as a Biocurator at Sagenome Pvt. Ltd., Thiruvananthapuram (May 15, 2024 – June 15, 2024). This role reinforced my commitment to leveraging my expertise to address pressing scientific challenges.
I believe that my research background and ability to work collaboratively in multidisciplinary teams will allow me to contribute meaningfully to your ongoing projects and research initiatives.
Thank you for your time and consideration. I look forward to the opportunity to contribute to your team and help drive forward the advancements in biotechnology research at your esteemed organization.
Sincerely,
Dr. Soumya A.

Sentamilarasi

Location: Virudhunagar -25, Tamil Nadu, India

Date of Birth: 12/08/2003

Research Experiences

Summer Internship

Department of Biotechnology, Bharathidasan University, Trichy-24, Tamil Nadu, India

• Worked under the guidance of Dr. Muthuselvam, Associate Professor, focusing on advanced molecular biology and analytical techniques.
• Gained hands-on experience in electrophoresis, SDS PAGE, and thin layer chromatography, contributing to technical expertise in analytical methodologies.

Training in Molecular Genetics of Neurodegenerative Disorders

CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), Delhi, India

• Undertook a five month research project under Dr. Mohammad Faruq, Senior Principal Scientist and Principal investigator.
• Investigated genetic variations related to neurodegenerative diseases, focusing ZFHX3-CGG repeats length variation in Indian patients with cerebellar ataxia.
• Acquired skills in genomic DNA isolation, PCR, agarose gel electrophoresis, Nanodrop, and fragment analysis using capillary electrophoresis.

Workshop

National Hands-On Workshop on Basic Cell Culture Techniques

Department of Biotechnology, Bharathidasan University, Trichy-24, Tamil Nadu, India

• Participated in hands-on workshop held from 14th-16th March 2022, gained practical hands-on experience in basic cell culture techniques.

Education

• [hidden] Biotechnology
• Bharathidasan University, Tiruchirappalli, Tamil Nadu, India
• CGPA: [hidden]
• Higher Secondary Certificate (HSC)
• MNRD Girls [hidden] School, Virudhunagar, Tamil Nadu, India
• Percentage: 82%
• Secondary School Leaving Certificate (SSLC)
• Sacred Heart Convent Girls High School, Virudhunagar, Tamil Nadu, India
• Percentage: 88%

Skills

Soft Skills: Excellent Communication and Writing

Technical Skills:

• Microbiology Techniques – Plating Techniques, Staining Techniques, Microscope
• Molecular Biology Techniques – DNA Isolation, Agarose Gel Electrophoresis, PCR, SDS PAGE, Nanodrop
• Molecular Genetics Techniques – Fragment Analysis-Capillary Electrophoresis
• GSA-Global Screening Array Technology
• Analytical Techniques – Thin Layer Chromatography
• Cell Culture – Basic Cell Culture Techniques
• Lab Instrumental Knowledge

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Cover Letter:

Sure, here is the redacted version of the text:

19 Feb 2025
Sentamilarasi
[hidden] Biotechnology
Redacted Email Address
Redacted Phone Number
Virudhunagar -626125
Tamil Nadu, India

Hiring Manager MOLPATH Pvt. Ltd Tamil Nadu

Dear Hiring Manager,

I am writing to express my keen interest in the Junior Research Scientist position at MOLPATH Pvt. Ltd., as advertised through the placement team of Biotecnika. With a Bachelor of Technology in Biotechnology from Bharathidasan University and hands-on experience in molecular biology and genetic analysis, I am eager to contribute my skills to your esteemed organization.

During my academic and research tenure, I had the privilege of conducting my undergraduate dissertation at CSIR-IGIB, focusing on the molecular genetics of neurodegenerative diseases. This experience honed my expertise in genomic DNA isolation, PCR, agarose gel electrophoresis, fragment analysis (capillary electrophoresis), and global screening array (GSA) technology. Additionally, I have acquired experience in SDS Page, Fundamental cell culture techniques through internship and national hands-on workshop.

MOLPATH’s commitment to advancing molecular pathology aligns with my passion for translational research and biotechnology. My analytical mindset, coupled with proficiency in laboratory techniques and data interpretation, positions me to contribute effectively to your research endeavors. I am particularly excited about the opportunity to apply my expertise in molecular diagnostics and contribute to cutting-edge research that drives advancements in healthcare.

I am eager to discuss how my qualifications align with your team’s objectives. Thank you for your time and consideration. I look forward to the possibility of contributing to MOLPATH’s mission.

Sincerely, Sentamilarasi

Introduction Summary:

Name: Bindumadhuri Cavuturu

Qualification: [hidden] in Biotechnology – Drug discovery

Summary: Enthusiastic, Hardworking, and Sincere individual with an immense interest in establishing a career in Life Science research.

Work Experience:

Research Associate-I – National Institute of Pathology, Delhi

April 2023 – August 2024

Project: Screening of Inhibitory Molecules against KIFC1, KIF4A, KIF15, and KIF20A for Breast Cancer Cells

Activities: In-silico screening, MD simulations, MTT assays

Junior Research Analyst – Excelra Knowledge Private Solutions, Hyderabad

March 2021 – March 2023

Education:

[hidden] in Biotechnology – Drug discovery

Pondicherry University, Pondicherry (2012 – 2021)

[hidden] in Biomedical Genetics

Vellore Institute of Technology, Vellore (2009 – 2011)

Achievements:

Publications:

1. Title: Molecular dynamics of interaction of Sesamin and related compounds with the cancer marker β-catenin – Journal of Biomolecular Structure and Dynamics

2. Title: Stabilizers influence drug-polymer interactions and physicochemical properties of disulfiram-loaded poly-lactide-co-glycolide nanoparticles – Future Science Oa

3. Title: Disulfiram and disulfiram-loaded poly-[lactide-co-glycolic acid] nanoparticles modulate metastatic markers and proteasomal activity in hepatocarcinoma Hep3bcell line – [hidden] Nanomed

Conferences Attended:

1. Oral presentation in 36th Annual conference of Indian Association of Bio-medical Scientists

2. Intensive “In-house Training in Animal Cell Culture Techniques”

Contact Details of Referees:

1. Dr. N. Arumugam

Email: [hidden]

Telephone: [hidden]

2. Dr. R. Amutha

Email: [hidden]

Telephone: [hidden]

3. Prof. N. Sakthivel

Email: [hidden]

Telephone: [hidden]

Skills:

Cell Culture, Western Blot, PCR, Human DNA Isolation, Docking, Molecular Dynamic Simulation Studies, Basic Microbial Technologies, Postgraduate Level Skills in Karyotyping

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Cover Letter:

I earned my [hidden] in Biotechnology in February 2021, focusing on drug discovery. My doctoral research centered on targeting β-catenin, a critical driver in 80% of sporadic colorectal cancers, through sesamin and its analogs. By leveraging rigid docking and molecular dynamics (MD) simulations, I identified potent inhibitors, which were subsequently validated through cell-based assays, flow cytometry, and Western blotting in SW480 and HT29 cell lines. This interdisciplinary approach reinforced my ability to bridge computational predictions with experimental validation.

Building upon this foundation, my postdoctoral research expanded my expertise in virtual screening using Schrödinger software and database mining through DrugBank, NCI natural database, and IBS natural compound database. Additionally, I developed doxorubicin-resistant MDA-MB-231 cell lines and conducted cytotoxicity assays to evaluate novel compounds against both standard and drug-resistant cancer cells.

I am confident that my experience aligns with the mission of your team, and I am enthusiastic about the opportunity to contribute to groundbreaking research in this field.

I welcome the opportunity to discuss how my expertise can complement your ongoing research. Thank you for your time and consideration.

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Cover Letter:

As a passionate Microbiologist with a strong academic background and research experience, I am excited to explore opportunities in an organization that leverage my expertise in microbiology and artificial intelligence.

With a Master’s degree in Microbiology and experience as an Assistant Professor in Microbiology at Annasaheb Magar College, Pune, India. I possess a deep understanding of microbial principles, laboratory techniques, and research methodologies. My teaching experience has also honed my skills in communicating complex scientific concepts to diverse audiences.

Recently, I completed a project on “Breast Cancer Detection using Support Vector Machine (SVM) Classifier” that integrated my microbiology background with artificial intelligence techniques. This project not only demonstrated my ability to work at the intersection of biology and AI but also sparked my interest in exploring the vast potential of AI in biomedical research.

I am now eager to transition into a research-focused role in an institute that values interdisciplinary approaches to scientific inquiry. I am confident that my unique blend of microbiology expertise and AI skills would enable me to make meaningful contributions to your research team.

I will be much obliged if you give me the chance to be a part of your organization.

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About Me:

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Experienced marketing professional with a proven track record of generating leads and increasing brand visibility. Passionate about developing creative marketing strategies to drive business growth.

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Work Experience:

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Marketing Manager, XYZ Company
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• Developed and implemented successful marketing campaigns that increased customer engagement by 30%

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• Managed a team of 5 marketing specialists and oversaw all marketing initiatives

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Marketing Specialist, ABC Corporation
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• Executed social media campaigns that doubled online followers within 6 months

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• Collaborated with sales team to create targeted marketing materials

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Education:

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Bachelor of Business Administration
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University of Marketing, Year

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Skills:

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Marketing Strategy, Social Media Management, Content Creation, Team Leadership, Data Analysis

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Cover Letter:

My college thesis project was titled- ‘Vaccine Design Against Ebola Virus Glycoprotein Using In-silico Approaches’ in which the primary goal was to strategize a potential vaccine design against Ebola virus variants using in-silico approaches. We identified a glycoprotein sequence which was similar in two Ebola variants and its antigenicity was checked using VaxiJen [hidden] which proved that the target sequence was antigenic in nature. Physicochemical and Structural analysis was performed and it was concluded that the selected target protein can be used as a best possible way to develop into a vaccine and for further research. My dissertation project at BHABHA ATOMIC RESEARCH CENTRE was- ‘To Investigate The Effect Of Redox Modifications On Functions Of [hidden] Cells’. I had hands-on training in various Animal tissue culture techniques like passaging, subculturing, thawing, freezing and cryopreservation, Maintenance and have used various high-tech instruments like IncuCyte, Flow cytometer, GelDoc, HTS analyzer, Microplate reader, Lyophilizer and BSL and animal hood. I have performed various phagocytosis assays, efferocytosis assays, PI staining, CFSE staining, DCFDA staining, NO assay, Clonogenic assay, MTT assay, ELISA, AGE, PAGE, RT-PCR, Cell cycle analysis, Bradford assay, Imaging. I am well-versed in using different cell-lines and their handling and storing them by making cryo vials. Apart from this I also had training in animal handling and mice dissection of spleen, intestine and bone marrow of BALB/c and Swiss mice. My project included 2 antioxidants (Chlorophyllin and Baicalein) and 2 prooxidants (Plumbagin and Mitocurcumin) and their modulations on the different functions of RAW cells [hidden] macrophages and how this could be applied further if there is a malfunctioning of these cells. I had my internship training in Apollo Diagnostics and had hands on training in RT-PCR techniques for covid-19 and Manual and automated RNA isolation techniques and data entry operations. I am currently working as a Project Assistant on an ICMR funded project- “Immunometabolomics in the quest for comprehending protection and pathogenesis in tuberculosis”. My work involves understanding metabolite profiles from patient immune cells, comparing them with healthy individuals and those with latent TB. In this role, I have gained hands-on experience in patient blood collection, Density gradient centrifugation, PBMC’s isolation and culture maintenance, Metabolite extraction, analyzing mass spectrum results, Isolation of T-cells by Magnetic associated cell separation, T-cell expansion, Intracellular staining for Flow cytometry, as well as REMA assay, Heat induced Hemolysis and Docking. I am a quick learner who thrives in hands-on laboratory environments and am eager to expand my knowledge in microfluidics and 3D cancer modelling. My strong analytical skills, attention to detail, and ability to adapt to interdisciplinary research make me a perfect fit for this role.

MAYA MATHEW, [hidden]

BIOTECHNOLOGIST/MICROBIOLOGIST

PROFILE

I am a [hidden] holder in Biotechnology from Mahatma Gandhi University at the School of Biosciences. I am a Biotechnologist with expertise in Molecular Biology, Microbiology and Nanocomposites with 7 peer-reviewed publications in the field and 5 are under processing. I have edited 2 books in “Antimicrobial resistance” with Apple academic press and also have 2 book chapters in the respective area.

WORK EXPERIENCE

2015 – 2019

St. Xavier’s College for Women, Aluva, Cochin

Assistant Professor – Department of Microbiology

2011 – 2014

VWR Lab Products PVT. LTD, Coimbatore

Process Associate – Cross Reference- EU (Life Sciences)

Handling life-science laboratory products supply chain management business across European countries by cross referencing laboratory products to customers. Coordinating with the team in India with that of Netherlands & Belgium regarding the laboratory products. Teaching Microbiology postgraduate students on theory and practical courses based on the syllabus prepared by the university. Syllabus revision & practical examination board member of Mahatma Gandhi University Kottayam. Isolation and identification of bacteria from veterinary samples, Culture media preparation, sterilisation, inoculation, plating, biochemical tests, Vitek analysis, DNA Isolation, PCR, Biofilm formation analysis, Characterization of the Microbiome, WGS analysis, Nano formulation synthesis, Nanocomposites- films for packaging applications, Characterization studies- FESEM, TEM, XRD, FTIR, UV-visible spectroscopy.

EDUCATION

2020 – 2024

Mahatma Gandhi University, Kottayam

[hidden] Scholar

2009 – 2011

Bharathiar University, Coimbatore

MSc Biotechnology

2006 – 2009

Mahatma Gandhi University, Kottayam

BSc Biotechnology

Thesis title: Phenotypic and molecular characterization of multidrug resistant mastitogens from cow milk and its nanotherapeutic management. Thesis title: Ameliorative effect of Averrhoa carambola extract on synergistic toxicity produced by Acetaminophen and Metformin.

RESEARCH EXPERIENCES

Google Scholar: [hidden]“>Maya Mathew ResearchGate

ORCiD: [hidden]-623X

SKILLS

Nucleic Acid Purification, Molecular Biology, Microbiology, Whole Genome Sequencing, Bioinformatics, Phage Annotation, PHASTER, RAST, CARD Database, Imaging Techniques, English (Fluent), Malayalam (Fluent), Tamil (Basic), Hindi (Intermediate)

“`

Cover Letter:

Dear Hiring Team,

I am writing to express my interest in the Research Scientist / Junior Research Fellow position at Molpath Private Limited, as advertised. With a strong background in cell biology and hands-on experience in cell culture, I am eager to contribute my skills and expertise to your innovative molecular diagnostics and research company in Vellore, Tamil Nadu. My enthusiasm for advancing microfluidic systems for cell culture, 3D cancer modeling, and drug screening applications aligns perfectly with Molpath’s commitment to pioneering research in molecular biology, biotechnology, and precision medicine.

I have a proven track record of maintaining detailed experimental records and contributing to scientific publications. My proficiency in cell biology techniques, including cell viability assays and microscopy, combined with strong analytical and problem-solving skills, makes me confident in my ability to excel in this role.

I am particularly drawn to Molpath’s focus on bridging the gap between cutting-edge technology and real-world clinical applications. The opportunity to work on a DBT-BIRAC-supported project and potentially transition into a PhD program is especially exciting, as it aligns with my long-term career goals in translational medicine. I am confident that my skills, experience, and passion for research make me an ideal candidate for this position.

Thank you for considering my application. I have attached my CV for your review and welcome the opportunity to discuss my qualifications further.

Sincerely,
Dr. Maya Mathew

Dr. M. ANGEL NIVYA

Indian nationality

Summary

A dedicated and experienced researcher with a [hidden] in Biotechnology and a strong background in protein purification, molecular biology, and biophysical techniques. Proficient in conducting research and publishing findings in reputable journals.

Professional Experience

Project Scientist II (March 2024 – June 2024)
Translational Health Science and Technology Institute (THSTI), Faridabad, India

Research Associate (April 2023 – February 2024)
Institute of Genomics and Integrative Biology (IGIB), New Delhi, India

Research Associate 1 (March 2019 – April 2022)
Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India

Education

[hidden] in Biotechnology, 2014 – 2018
Indian Institute of Technology Hyderabad (IITH), Hyderabad, India

[hidden] in Biotechnology, 2009 – 2011
St. Joseph’s College, Tiruchirappalli, Tamilnadu, India

[hidden] in Biotechnology, 2006 – 2009
[hidden] Arts College, Thanjavur, Tamilnadu, India

Skills

Protein Skills: Cloning, Expression, and Purification of Proteins, SDS-PAGE, Western blot, Bioinformatics

Molecular Biology Skills: Plasmid preparation, Transformation, PCR, DNA sequencing, RT-PCR

Biophysical Techniques: Fluorescence Polarization, Isothermal Titration Calorimetry

Computational Skills: Molecular Modelling, Docking, Simulation, Sequence Analysis

Research Experience

Peptides based Drugs: Synthesis of Peptides at Indian Institute of Science

Plant Molecular Biology: Trained at the Rajiv Gandhi Center for Biotechnology

CoVID19: Involved in Cloning and Expression of Proteins

Publications

• Tanneru N., Nivya MA., et al. (2023). Plasmodium DDI1 – International Journal for Parasitology.
• Sudhakar R., Nivya MA., et al. (2022). Antibody response in SARS-CoV-2-infected individuals – Indian Journal Medical Research.

References

Prof. Rajakumara Eerappa
Professor and HOD
Dept. of Biotechnology, IIT Hyderabad
Email: [hidden]

Prof. Puran Singh Sijwali
Sr. Principal Scientist
Centre for Cellular and Molecular Biology (CCMB)
Email: [hidden]

Prof. Archana Singh
Principal Scientist
Institute of Genomics and Integrative Biology (IGIB)
Email: [hidden]

Prof. Milan Surjit
Professor
Translational Health science and Technology Institute (THSTI)
Email: [hidden]

Honors & Awards

3rd position in 10th Class
Qualified Graduate Aptitude Test in Engineering (GATE-2014)

Work Style

Strong independent work style with excellent team work skills. Passionate and well-organized.

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Cover Letter:

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Dear Sir,
I am M. Angel Nivya, a graduate student of Prof. Rajakumara Eerappa from the Indian Institute of Technology (IIT) Hyderabad, India. I had a working experience of 3 years in the Centre for cellular and molecular biology (CCMB) under the guidance of Dr. Puran Singh Sijwali and worked as a research associate in the Institute of Genomics and Integrative Biology (IGIB) under the guidance of Dr. Archana Singh for one year. I was working as Project scientist II in the Translational Health Science and Technological Institute (THSTI) under the guidance of Dr Milan Surjit.
Research experience from [hidden] expertise (in research) belongs to the cloning and purification of proteins and their biophysical and computational approaches to investigate the molecular interactions of proteins to histone peptides. Most of my [hidden] research work focused on the UHRF1 (Ubiquitin-like, containing PHD and RING finger domain). We have demonstrated that the binding affinities of UHRF1 TTD exhibit higher binding affinity towardsH3K9me2 compared to H3K9me1/3through isothermal titration calorimetry (ITC). We have employed a computational tool, Molecular Dynamic (MD) simulation, to characterize the methyl status-specific binding of H3R2K9me by the TTD-PHD, and to analyze the effects of H3K4 di-methylation on H3R2K9me recognition. We have validated the MD simulation data through ITC binding study. These studies establish that the UHRF1 TTD-PHD exhibits lower binding free energy (ΔGbind) towards H3R2K9me2 compared to H3R2K9me3. We also showed that the SRA domain of UHRF1 binds to all 5mC oxidation derivatives.
Research Associate: In CCMB, I was working on biochemical and biophysical characterization of DNA Damage inducible (DDi1) protein from Plasmodium falciparum. Ddi1 is a retroviral aspartyl protease and is involved in the repair of DNA-protein crosslink. Ddi1 also acts as a proteasome shuttle factor. We investigated the Plasmodium DDI1 to identify its roles during parasite development and potential as a therapeutic target. We have also knocked in pfDDi 1 into Saccharomyces cerevisiae to investigate the growth effect.
In IGIB, I was working on isolation of Keratinocytes, Melanocytes and Fibroblast cells from foreskin patient samples and Cloning, Expression and purification of Transglutaminase 1(TGM1) protein from [hidden]
In THSTI, I was involved in the Development of a recombinant vaccine against Hepatitis E Virus and their immunological characterization.
Skills in Brief: I have also trained in molecular biology, biochemistry, and structural biology related techniques and also experienced in mammalian cell culture, Saccharomyces cerevisiae and Pichia pastoris. I am very well experienced in protein biochemistry, biochemical and biophysical characterization of proteins and epigenetics related concepts and experiments.
Research Interest: I am interested in DNA- protein, RNA- Protein and peptide-protein interactions, protein biochemistry, Structural biology and Epigenetics. My main goal is to setup independent research lab in good academic institute.
I believe that based on my research experience and skill set gained, I would perform to the best of ability for the given project. I am a highly motivated, innovative individual who works well independently as well as collaboratively on teams of scientists from various backgrounds. I also had experience in mentoring and training both undergraduate and postgraduate students with little prior research skills.
Thank you.
Sincerely,
Dr. M. Angel Nivya.