CV

Profile Summary

An enthusiastic researcher having extensive experience in research of Micro Biology. Vital initiatives on adapting new skills, novel technology, and implementing new protocols. Proven ability to manage multiple projects and meet critical deadlines in a team.

Research Experience

Andhra University College of Engineering (AUCE), India

• December 2019 – April 2023
• ❖ Worked as a Full Time Research Scholar in Biotechnology Department for 4 Course works & 1 Lab work.
• ❖ Demonstrated in Industrial Biotech products, Genetics, Plant Tissue Culture, Biology subjects and Biochemistry & Bioanalytical Techniques Lab Experiments for practical laboratory for undergraduate students.
• ❖ Collaborated with other researchers to conduct multiple projects.
• ❖ Involved in scientific papers, grants and thesis writing.
• ❖ Research outcomes have been published in reputed journals.
• ❖ Presented my research work at multiple national and international conferences.
• ❖ Assisted undergraduate, postgraduate and co-researchers with their research work.

Biotech Consortium India Limited (BCIL), New Delhi, India

• December 2017 – June 2018
• ❖ Worked as Biotech Industrial Research Trainee in the project “Analytical method validation of Chemical Instrumental and Microbial Analysis of Semi-Synthetic Penicillin’s – Ampicillin Trihydrate” carried in Aurobindo Pharma Ltd., Vizag.
• ❖ Performed in High-Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC) instruments.
• ❖ A Hypersil ODS column of length 250mm x 4.6 x 5micron was used.
• ❖ Gained experience in handling and operating instruments like HPLC, GC, IR, SOR, and UV Spectrophotometer.
• ❖ Gained practical knowledge in chemical analysis.
• ❖ Obtained knowledge about different procedures involved in releasing an API by QA.
• ❖ Learned techniques involved in Microbiological Analysis including Microbial Limit Test and Identification of pathogenic organisms in purified water and various products.

Education

• PhD
• 2018-2024
• Department of Chemical Engineering, Center of Biotechnology, Andhra University College of Engineering (A), Andhra University, Visakhapatnam, Andhra Pradesh., India
• Title of Thesis: – “Characterization of a Novel Marine Actinomycete: Nocardiopsis Dassonvillei for Production of Extracellular Lipase”.
• Supervisor: – Lt. Dr. N.M. Yugandhar, Associate Professor, Department of Chemical Engineering, Andhra University College of Engineering, Andhra Pradesh, India

• M.Tech.
• 2014-2016
• Department of Chemical Engineering, Center of Biotechnology, Andhra University College of Engineering (A), Andhra University, Visakhapatnam, Andhra Pradesh., India
• Title: – “Production of Lovastatin under Solid State Fermentation using Fungal Isolate from Soil Sample”.
• Supervisor: – Lt. Dr. N.M. Yugandhar, Assistant Professor, Department of Chemical Engineering, Andhra University College of Engineering, Andhra Pradesh, India

• B.Tech.
• 2010-2014
• Department of Biotechnology, National Institute of Technology, Warangal (N.I.T.W), Andhra Pradesh, India
• Project: – “Bioinformatics analysis of ‘GXSXG’ class of lipases and in-silico design of novel lipases toward lipolytic activity”.

Publications

1. Praveen Krishna, V., Bala Tejeswararao, V., Subhakanta, S., Naveen Kumar, K., Charan Teja, P. (2024). Isolation, Identification and Screening of Potential Marine Actinomycete for Lipase Production under Submerged Fermentation by using Tributyrin substrate. International Journal of All Research Education and Scientific Methods, 12: 3415-3424. Link
2. Bala Tejeswararao, V & Praveen Krishna, V. (2023). Studies on Production and optimization of novel α-amylase by using wheat bran residue from Streptomyces enissocaesilis under solid state fermentation. International Journal of Advances in Engineering and Management, 5: 579-582. DOI: Link
3. Bala Tejeswararao, V & Praveen Krishna, V. (2023). Partial Purification and Immobilization of novel α-amylase from streptomyces enissocaesilis. International Journal of Pharmaceutical Research and Applications, 8: 2911-2913 DOI: Link

Presentations

• Presented a Technical Paper Presentation entitled “Optimization of Lipase enzyme production by a newly isolated species” at the National Level Youth Festival on Vignan Mahotsav 2013 at Vignan University, Vadlamudi, Guntur – 522213, Andhra Pradesh, India (4th – 5th January 2013).
• Presented a Poster entitled “Isolation, Production and Optimization of Antimicrobial Metabolite from Marine Actinomycetes” at National Conference on Recent Advances in Chemical Engineering Ripples 2K20 organized by Department of Chemical Engineering, Andhra University, Visakhapatnam, India (21st – 22nd January 2020).
• Presented a Virtual Paper Presentation entitled “Isolation and Screening of Cellulolytic Actinomycetes from marine freshwater by using submerged fermentation” at the International Conference (Virtual) on Affordable Strategies for Health, Environment and Food Technology organized by the Department of Biotechnology Engineering, N.M.A.M. Institute of Technology, Nitte, Karkala Taluk, Karnataka – 574110, India (22nd – 23rd December 2020).
• Presented a Paper Presentation on “Isolation and Screening of Lovastatin production using fungal isolate from soil samples under solid state fermentation” and got the “Best Oral Presentation Award” in Two-Day Online National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental Biotechnology (NCB4EBT-2021) conducted by the Department of Biotechnology, National Institute of Technology, Warangal-506004 (29th – 30th January 2021).

Workshops

1. Attended an Event in MEGALITH 2013, on “IEEE Publications: Technical Writing and Enhancing Your Research” the Annual Civil Engineering Tech-Fest of IIT Kharagpur conducted by the Department of Civil Engineering, Indian Institute of Technology Kharagpur held during 15th – 17th March 2013.
2. Attended a National Webinar on “Trends in Biotechnology” at the Department of Chemical Engineering, AUCE (A), Andhra University, Visakhapatnam held on 28th – 29th September 2020.
3. Attended an International Webinar on “BIORENAISSANCE” at the Department of Chemical Engineering, AUCE (A), Andhra University, Visakhapatnam held on 10th – 11th November 2021.
4. Carried out 2 weeks of Faculty Development Program in “Research Methodology & Biomedical Production Engineering” Organized by Andhra Pradesh MedTech Zone (AMTZ), Sponsored under “Skill Vigyan” Scheme of Department of Biotechnology, Government of India held during 6th -18th December 2021.
5. Attended a day National Workshop on “Recent Advances in Chemical Engineering” organized by Department of Chemical Engineering, AUCE (A), Andhra University, Visakhapatnam held on 31st May 2023.

Skills

• Microbiology: Isolation of microorganisms, biochemical and molecular identification of microorganisms, preservation and maintenance of microorganisms.
• Cell culture: Media preparation and sterilization, cryopreservation and revival of cells, cell counting, cell viability assay, virus isolation and titration.
• Instrument Techniques: Gained knowledge in chromatographic techniques -HPLC/GC-MS/LC-MS during the project.
• Bioinformatics: NCBI tools, gene identification, primer designing, multiple sequence alignment, phylogenetic tree construction, bioinformatic tools and software (Mega X, Bioedit), sequence analysis, PDB database, homology modelling.
• Molecular Biology: Nucleic acid isolation, cDNA synthesis, PCR, RT-PCR, cloning in pET vectors, expression optimization in E. coli, size exclusion chromatography, western blotting, ELISA, and zymogram.
• Soft skills: MS Office tools, documentation, and technical writing.

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