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Cover Letter:

**To, 01.07.2025**
**The Hiring Manager,**
**D-NOME Biosciences, Hyderabad**
**Dear Sir/Mam**,
I am writing to express my keen interest in the Scientist Position at D-NOME Biosciences, as advertised. With a Ph.D. in Cancer Biology (thesis submitted, NIT Durgapur), a strong publication record, and hands-on experience in molecular and cellular biology techniques, I am confident in my ability to contribute meaningfully to your ongoing scientific research initiatives.
During my doctoral research at the Department of Biotechnology, NIT Durgapur, I investigated the regulatory mechanisms and functional implications of the anti-inflammatory gene **15-Lipoxygenase** in cancer biology. This work led to co-authorship on several peer-reviewed publications in high-impact journals such as _Cellular Immunology_ and _Free Radical Biology and Medicine_. These experiences have given me a robust foundation in experimental design, data analysis, and scientific communication.
My core strengths lie in mammalian cell culture, Western blotting, qPCR, transfection techniques, and data interpretation using tools such as GraphPad Prism and MS Excel. My adaptability, teamwork, and constant eagerness to learn new techniques and skills have allowed me to contribute effectively in dynamic and diverse scientific environments and I am passionate about translating research into tangible outcomes that can drive innovation and impact. I have also demonstrated strong collaboration and leadership skills by mentoring over five undergraduate and postgraduate students, ensuring knowledge transfer and project continuity.
I am enthusiastic about the opportunity to contribute to cutting-edge scientific projects as part of your esteemed company. I am confident that my technical proficiency, research acumen, and commitment to excellence would make me a valuable addition to your team. I am particularly drawn to join for Scientist Position at D-NOME Biosciences so that I can apply my scientific background, problem-solving abilities, and passion for research to support your team’s objectives.
Thank you for considering my application. I am available at your convenience for an interview.
Warm Regards,
**Surbhi Swaroop**
** **

AKANKSHA SINGH

New Delhi, IN

OBJECTIVE

Result-driven graduate with knowledge of molecular techniques seeking full-time roles in Biotechnology, Molecular Biology, and its application.

EXPERIENCE

DBT Intern | Advanced Genomics Institute and Laboratory Medicine (AGILE)

JUNE 2024 – DECEMBER 2024

Gained hands-on experience in molecular biology techniques, including genome extraction from human samples and RT-PCR analysis for disease diagnosis.

Developed proficiency in laboratory protocols and data analysis for accurate disease diagnosis.

Project Trainee | ICAR- National Bureau of Plant Genetic Resources

JANUARY 2021- JUNE 2021

Genetic Diversity analysis of naked Barley (Hordeum vulgare L.) germplasm using SSRs marker

• Genomic DNA extracted manually.

• Genotyping using Thermocycler (PCR)

• Heterozygosity calculated using Genalex 6.5 software

Trainee | ICAR- National Bureau of Agriculturally Important Microorganism

MAY 2019 – JULY 2021

Molecular Characterization of U. virens that cause false in Rice

• Fungi genomic DNA extracted manually using CTAB extraction buffer (20 different fungi were used in this study).

• Genotyping using Thermocycler (PCR).

• Characterization analysis using horizontal Dendrogram.

Trainee | MRD Life Sciences- Lucknow

DECEMBER 2018 – JANUARY 2019

Basic Molecular Techniques

• DNA extraction methods.

• Introduce to various equipment of the laboratory.

• Quantification and qualification of DNA, RNA, and Proteins

EDUCATION

Masters of Technology in Biotechnology | Guru Gobind Singh Indraprastha University

2022 – 2024

GPA- 8.04/10

Bachelor of Technology in Biotechnology | Sam Higginbottom Institute of Agriculture Technology and Sciences- DU

2017 – 2021

GPA- 7.4/10

SKILLS

Molecular Techniques: DNA/RNA Extraction (CTAB method, TRIzol method, Phenol-Chloroform method, SDS extraction method), PCR (standard PCR, RT-PCR), Gel electrophoresis (agarose gel, polyacrylamide gels), Cell culture

Protein Analysis: SDS-PAGE, Protein quantification (Bradford assay, BCA assay), Protein purification

Data Analysis: Image analysis (ImageJ), Statistical analysis, Data visualization (MS Excel)

ACTIVITIES

WORKSHOP: “Using Ibin Databases for Research And Education In Ecology And Conservation” (CSIR- CIMAP) 2019 Lucknow, Uttar Pradesh, India

CONFERENCES: National Conference (Biomillap) On “Recent Advances In Agriculture, Food Tech And Human health” (Shiats-DU)- 2019 Prayagraj, Uttar Pradesh, India

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Cover Letter:

With a postgraduate degree in Biotechnology and hands-on experience in molecular biology and genomics workflows, I am passionate about contributing to impactful scientific research and discovery. During my academic journey and research training, I have worked extensively with DNA/RNA extraction methods (manual and automated), PCR and RT-PCR, electrophoresis, and molecular marker analysis. My work at various research institutes has also familiarized me with genotyping, sequence analysis tools, and basic concepts of Next-Generation Sequencing (NGS). These experiences have strengthened my technical foundation and nurtured my deep interest in lab-based research and innovation. I am particularly drawn to roles that involve method development, protocol optimization, or molecular diagnostics, where I can apply my skills and continue to grow in a collaborative and research-focused environment. I bring a disciplined work ethic, attention to detail, and an eagerness to contribute to meaningful scientific advancements.

**Medha Gupta**
Medical Biotechnologist

**Summary:**
Dedicated and detail-oriented Medical Biotechnologist with hands-on experience in molecular biology, cell culture, protein biochemistry, and microbiology. Passionate about translational research in infectious diseases, drug discovery, and clinical diagnostics. Proven ability to design and execute experiments, analyze data, and contribute to innovative research.

**EDUCATION**
MSc. Medical Biotechnology
Bharati Vidyapeeth, Pune
07/2021 – 05/2023
CGPA: 9.3
Thesis: Determining GAP activity of TBC domain-containing protein against small G-proteins

BSc. Medical Biotechnology
Amity University, Noida
06/2018 – 05/2021
CGPA: 8.71
Thesis: Prevalence of etiological organisms in Urinary Tract Infections and its correlation with urinalysis

**WORK EXPERIENCE**
Project Associate
CSIR-Institute of Microbial Technology (IMTech)
09/2023 – 10/2024
Chandigarh
Tasks:
– Investigated protein-protein interactions of small G-proteins using advanced biochemical assays.
– Performed protein purification, Western blotting, co-immunoprecipitation, pulldown assays, yeast two-hybrid assays, and immunostaining for confocal microscopy.
– Maintained and sub-cultured mammalian cell lines (HeLa, HEK293T) for experimental studies.
– Contributed to research on intracellular signaling mechanisms relevant to lysosomal secretion and autophagy.

**CONFERENCES**
– International e-Conference on frontiers in Industrial Biotechnology (ICFIBT-2020)
– Next Generation Biomaterials for Drug Discovery / 2020
– Advanced Training on Microbial Technology /2021
– Clinical aspects of Endocrinology in Ayurveda /2019

**ADVANCED COURSES**
Drug Hunting: The Science of Making New Medicines – Specialization (Davidson College & Novartis)
– Introduction to Drug Hunting, Target, Assays, and Screening
– Pharmacokinetics, Preclinical Safety, Lead Selection and Optimization, Pre-formulation

**LANGUAGES**
– English: Full Professional Proficiency
– Hindi: Native or Bilingual Proficiency

**Skills:**
– Protein Biochemistry
– Cell Culture & Imaging
– Lab Management
– Protein purification
– Co-immunoprecipitation
– Pathogen identification
– Antibiotic resistance profiling
– Confocal Microscopy
– Plasmid isolation
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Cover Letter:

Dear Hiring Manager,
I am writing to express my keen interest in the Molecular Biology Scientist position at D-NOME Biosciences. With a Master’s degree in Medical Biotechnology and hands-on experience in molecular biology and protein biochemistry, I am enthusiastic about the opportunity to contribute to your innovative research endeavors.
During my tenure as a Project Associate at CSIR–Institute of Microbial Technology (IMTech), I was involved in projects focusing on protein–protein interactions of small G-proteins, which are pivotal in cellular signaling pathways. My responsibilities included:
– Protein Purification and Analysis: Executed protocols for purifying proteins and assessing their interactions using techniques like Western blotting and co-immunoprecipitation.
– Molecular Cloning: Designed and constructed plasmids for gene expression studies, facilitating the investigation of protein functions.
– Cell Culture: Maintained and manipulated mammalian cell lines (HeLa and HEK293T) for transfection and expression studies.
– Microscopy: Utilized confocal microscopy to visualize cellular processes and protein localization.
These experiences have honed my technical skills and deepened my understanding of molecular mechanisms, aligning well with the requirements of the Molecular Biology Scientist role at your esteemed organization.
What particularly draws me to D-NOME Biosciences is your commitment to advancing molecular diagnostics and therapeutic solutions. I am eager to apply my expertise to contribute to your ongoing projects and collaborate with a team dedicated to scientific excellence.
I would welcome the opportunity to discuss how my background and skills can benefit D-NOME Biosciences. Thank you for considering my application.
Sincerely,
Medha Gupta

Nikita Thakur

Date of Birth: 28/01/1999

Marital Status: Married

Nationality: Indian

Objective

Diligent candidate with diverse expertise in biochemistry, molecular biology, immunology, and microbiology, combined with strong communication skills to engage effectively across all levels of an organization.

Education

MSc Biotechnology
Ramnarain Ruia Autonomous College, 2021
Percentage: 80.16%

BSc Biotechnology
HPT Arts and RYK Science College, 2019
Percentage: 79.55%

Soft Skills

• Good communication
• Quick learner
• Flexible and adaptable to changes

Audit Exposure

• WHO GMP
• OHSAS and EHS audits
• ISO 13485 and ISO 9001 audit

Non Technical Skills

• C language
• MS-CIT and MS Office
• Python Programming language
• Data visualization tools: Power BI and SQL

Experience

Proto 9 Material Private Limited

Research Assistant
26/03/2024 – 28/03/2025

Job responsibilities:

• Manage and supervise bacterial and fungal culture repositories.
• Conduct nanoparticle synthesis and comprehensive protein analysis.
• Execute media preparation and range of microbial procedures.
• Conduct accurate total microbial counts and spore counts.
• Utilize techniques to assess microbial diversity in soil.
• Perform various microbial tests and assays.

Yashraj Biotechnology

Executive
25/11/2021 – 22/03/2024

Worked on various projects and analytical techniques including method Validation, protein analysis, and Quality Management Systems.

Amar Products

Jr. Microbiologist
15/07/2021 – 23/11/2021

Job responsibilities:

• Operation and calibration of lab equipment.
• Responsible for environmental monitoring and quality control.
• Performed routine Microbiological Examination testing.
• Handled water sampling and testing.

Instruments Handled

PH meter, UV spectrophotometer, Incubators, Autoclave, Air sampler, HPLC, Real time PCR, Cell counter, Flow Cytometer, Microscopes, etc.

Declaration

I solemnly declare that the above information is true and to the best of my knowledge and belief.

Skills

Biotechnology, Research, Laboratory Techniques, Data Analysis, Quality Control, Biochemistry, Molecular Biology, Immunology, Microbiology, Microsoft Office, Python, Power BI, SQL

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Cover Letter:

I am Nikita Thakur having 3.8 years of experience. I have gained experience in various domains like biochemistry, microbiology, molecular biology, and immunology, in vitro assays, analytical techniques. I will be a good asset to the company if given a chance.

Dr. YASHVANT RAO

Scientific Laboratory Manager cum Nodal Officer

Sadar (District) Hospital Sahibganj-816109, Jharkhand, India

ACADEMIC CREDENTIALS

2018
Ph.D. (Nanosciences) from Center for Nano Sciences, Central University of Gujarat (CUG), Gandhinagar, Gujarat
Title: “Green synthesis and characterization of Gold Nano-particles (NPs) for anticancer drugs delivery”.

RESEARCH & EXPERIENCES

Sept 2024 – Present
Hospital Manager, Sadar Hospital Sahibganj, Jharkhand

SUBJECTS STUDIED

Ph.D. (Nano Sciences):
Worked on the project “Green synthesis and characterization of gold nano-particles for anticancer drugs delivery” completed.

EXECUTIVE SUMMARY

Presently working as Scientific Laboratory Manager cum Nodal Officer, New Sadar Hospital Sahibganj.

TECHNICAL EXPERT HANDS WITH INSTRUMENT

• ELISA, RT-PCR (Agilent Strata gene Mx30005P and BIORAD CFX96)
• Automated RNA Ex. (TULIP amplichain EX96, Hi-Media InstaNX Mag 96, KingFisher Duo Prime and ILS GEN Fast) Techniques

PROFESSIONAL AFFILIATIONS

• Life member of The International Nanoscience Community (TINC)
• Life member of Indian Science Congress Association (ISCA)

RELEVANT SKILLS

Protein Farmolution, Structural & Molecular Characterization, Molecular Medicine, Cytology, Cellular Biology, Nano-Drug Delivery, Medical Micorbiologist, Anti-cancer Research, Protein Farmolution

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Cover Letter:

I’m sorry but you haven’t provided any data for me to redact. Could you please provide the data so I can redact any email addresses, phone numbers, or LinkedIn profile URLs for you?

Introduction:

Data Analyst with expertise in Mycology, Antimicrobial drug discovery, Molecular Microbiology, and Protein Science (Biochemistry). Skilled in quantification of proteins and peptides, AFST studies, flow-cytometry analysis.

CV:

Education

Ph.D. (Antimicrobial Drug discovery) – Birla institute of technology and sciences, K.K. Birla Goa campus, India (2020-2025)

M.S. Pharm (Biotechnology) – National Institute of Pharmaceutical education and Research, Guwahati, Assam, India (2015-2017)

B. Pharmacy – JITS, affiliated to Jawaharlal Nehru Technological University, Kakinada, Andhra Pradesh, India (2012-2015)

Research Experience

Senior Research Fellow – Birla Institute of Technology and Sciences, K.K. Birla Goa campus, India (Aug 2023 to Dec 2024)

SERB-Junior Research Fellow – Birla Institute of Technology and Sciences, K.K. Birla Goa campus, India (Oct 2019 to Dec 2022)

Junior Research Fellow – National Institute of Science Education and Research, Odisha (Jan 2018 to Feb 2019)

Publications

• Madhuri, Madduri, Shivaprakash M. Rudramurthy, and Utpal Roy. “Bacillus-derived Lipopeptides Inhibit and Eradicate Candida tropicalis Biofilms: An in-Vitro Exploration” – Biofilm (2025)
• Madhuri, Madduri, Shivaprakash M. Rudramurthy, and Utpal Roy. “Two promising Bacillus-derived antifungal lipopeptide leads AF4 and AF5 and their combined effect with fluconazole on the in vitro Candida glabrata biofilms.” Frontiers in Pharmacology (2024)
• Madduri, Madhuri, Shivaprakash M. Rudramurthy, and Utpal Roy. “Two promising natural lipopeptides from Bacillus subtilis effectively induced membrane permeabilization in Candida glabrata.” Acta Biochimica Polonica (2024)
• R A Paul, Madduri Madhuri, Nisha K, Swetha R, Shivaprakash M, and U. Roy. “Efficacy of two novel antifungal lipopeptides, AF4 and AF5 of Bacillomycin D family in murine models of invasive candidiasis, cryptococcosis, and aspergillosis”. APMIS (2024)
• Ramesh, Swetha, Madhuri Madduri, Shivaprakash M. Rudramurthy, and Utpal Roy. “Functional characterization of a Bacillus-derived novel broad-spectrum antifungal lipopeptide variant against Candida tropicalis and Candida auris and unravelling its mode of action.” Microbiology Spectrum (2023)

Conference Proceedings

• Madhuri, M., Swetha, R., Rudramurthy, S.M. and Roy, U., 2022. P068 Inhibition of Candida glabrata biofilm using the combination of a novel antifungal cyclic lipopeptide and azole: An in vitro synergistic approach. Medical Mycology
• Ramesh, S., Shaw, D., Madduri, M., Rudramurthy, S. M., & Roy, U. (2022). P076. The promising antimycotic activities of a novel cyclic antifungal lipopeptide against Human Dermatophyte Isolates. Medical Mycology

Technical Skills

Microbiology: Preparation of media, maintain sterility, Handling (LAF, BSL II pathogens) yeast (Candida albicans, C. glabrata, C. tropicalis, C. parapsilosis, Aspergillus fumigatus. Cryptococcus neoformans.), and bacteria cultures E. coli, Pseudomonas sp., Staphylococcus sp. and cell culture, methods like in vitro studies.

Biofilm studies: Biofilm quantification methods like crystal Violet assays, viability assay, biofilm EPS estimation, FTIR analysis of biochemical compositions, Expertise in characterisation of anti-biofilm properties, including handling of mono or dual species, fungal biofilms through various techniques.

Microscopy: Biological sample preparation and analysis of scanning electron microscopy (SEM), instrument handling, Cell wall staining methods, sample preparation and analysis of confocal microscopy (CLSM) imaging, flow cytometer.

Mode of action studies: Antimicrobial mode of action like membrane permeability assays, ROS generation, and apoptosis markers like FITC-Annexin-V and tunnel assays, zeta potential.

Protein Science: Expertise in protein expression, purification and quantification methods and Isolation of bacteria secreted lipopeptides (secondary metabolites), and chromatography techniques like adsorption chromatography, affinity chromatography, size exclusion, SDS page, native page, acid precipitation, organic solvent extraction and protein characterization techniques.

Skills and Abilities

Analytical chemistry: Expertise in HPLC (analytical, semi-preparative, preparative scale) LC-MS and various instruments like FPLC, FTIR, UV spectrometer, multi-plate reader.

Computer Skills:

• Excellent communication skills- writing manuscripts and oral presentations.
• Working knowledge of scientific software (Mendely-Reference manager, and MS office)
• Confocal image software (Image J, Fiji, Comstat, Imaries)
• Flow cytometry data analysis (FlowJo)
• Ability to analyze data and problem-solving skills (Graph pad Prism, SPSS, Origin).
• Familiarity with programming languages such as python, and R program.

Academic achievements

• Qualified GPAT national exam in the year 2015 with an all India rank of 2542
• Qualified NIPER-JEE 2015 with an all India rank of 1225
• Best poster presentation for liposomes formulated drugs during the conduct of seminar on “recent trends in pharmacy and pharmacology on March-2015”.
• International entrepreneurship opportunity in Andhra Pradesh on December-2014.

References

Prof. Utpal Roy, Professor, Department of Biological Sciences, BITS-PILANI Goa Campus, India.

Prof. Vivek Rangarajan, Professor, Department of Chemical Engineering, BITS-PILANI Goa Campus, India.

Dr. Anthony Addlagatta, Chief Scientist, Indian Institute of Chemical Technology (IICT-Hyderabad), India.

Declaration

I hereby declare that all the information provided by me in this application is factual and correct to the best of my knowledge and belief.

Keywords

Data Analyst, Mycology, Antimicrobial drug discovery, Molecular Microbiology, Protein Science, AFST, Biochemistry, Flow-cytometry, Proteins, Peptides

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Cover Letter:

**Hiring Manager**
D-NOME Biosciences
Hyderabad
**Subject:** Application for Scientist – Molecular Biology
Dear Hiring Manager,
I am writing to express my interest in the Scientist – Molecular Biology position at D-NOME Biosciences, Hyderabad. I have recently submitted my Ph.D. thesis in the Department of Biological Sciences at BITS Pilani–Goa Campus, India, where I specialized in microbiology and mycology, focusing on antifungal drug discovery and mechanisms of resistance in _Candida_ species. Additionally, I hold a Master’s degree in Pharmaceutical Biotechnology from the National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, India.
During my doctoral research, I purified novel antifungal lipopeptides from _Bacillus_ species using multi-step purification methods, including supernatant collection, acid precipitation, organic solvent extraction, and adsorption chromatography. I further optimized purification processes from semi-preparative scale HPLC to preparative-scale HPLC and have expertise in additional purification techniques such as analytical HPLC, affinity chromatography, size-exclusion chromatography, ion-exchange chromatography, and LC-MS analysis.
In addition, I hold a Master’s degree in Pharmaceutical Biotechnology from the National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, India. As part of my Master’s dissertation, I have worked in the structural biology lab at IICT, Hyderabad. I gained hands-on experience in recombinant protein purification and hanging-drop crystallization techniques. I am particularly drawn to this recently advertised position role in study coordinator involving the development of SOPs for measuring biomolecules and biological fluids using various analytical techniques to explore new paradigms in nutrition research.
My interdisciplinary skill set, combined with a strong foundation in protein purification, biochemical analysis, and a passion for learning new analytical methods, positions me well to support and contribute meaningfully to this project. Enclosed are my CV as requested. I welcome the opportunity to discuss how my experience and enthusiasm can benefit your research team.
Thank you for considering my application.

DHRUVA KUMAR KESIREDDY

Hyderabad, Telangana

SUMMARY

Detail-oriented and skilled researcher with expertise in molecular biology, tissue processing, cell culture, immunohistochemistry, genomics, and chromatin accessibility assays. Strong background in lab operations, inventory management, and vendor coordination to ensure seamless workflow. Committed to driving scientific advancements through precision, efficiency, and innovative problem-solving in biotechnology and life sciences.

EDUCATION

2017 – 2020
Bachelors in Biotechnology, Microbiology and Chemistry
Keshav Memorial Institute of Commerce and Sciences – (affiliated to Osmania University), Hyderabad

2020-2022
Master’s in Animal Biology and Biotechnology
University of Hyderabad

WORK EXPERIENCE

2024 – Present
Project Associate I – CSIR – Centre for Cellular and Molecular Biology
Project Based Student Trainee, CSIR – Centre for Cellular and Molecular Biology

TECHNICAL SKILLS

• Molecular techniques: DNA isolation, Cut-and-Paste cloning, PCR-based cloning, Restriction digestion, Ligation, Transformation, Plasmid isolation (large and small quantities), Gel electrophoresis
• RNA isolation, cDNA preparation, qPCR
• Protein Techniques: Bicinchoninic Acid (BCA) assay, SDS-PAGE, Western blotting
• Tissue Processing: Embedding, slicing spinal cord and brain tissues, Immunohistochemistry, Microscopy techniques for histological analysis
• Cell Culture: Media preparation, Cell splitting, Immunofluorescence techniques
• Genomics: Nuclei Isolation, BULK-ATAC, CUT&RUN, Hi-C
• Software Skills: MS Word, MS Excel, MS PowerPoint, R programming for Data analysis and Visualization

LAB OPERATIONS SUPPORT

• Adeno Associated Virus (AAV) Production: Maintenance of HEK293T cells, Transfection, AAV Production, Cell Harvesting, AAV Purification, Titering, quality control

CERTIFICATIONS

• CMMDR2024 and IAN 2024 poster presentation

INTERNSHIP

Drug Discovery and Development, under the guidance of Dr. Mirza S Baig, IIT Indore

ACTIVITIES

• Organized and managed a stall at CCMB Open Day twice, educating children on Neurobiology

ACHIEVEMENTS

• Awarded Best Guide Prize at the Open Day events conducted by CSIR-CCMB (2023 & 2024)
• Received four gold medals during graduation
• Qualified national entrances like GAT-B and the University of Hyderabad entrance, TS-SET
• 2nd prize in PowerPoint presentation on International Science Day (KMICS)
• 1st prize in poster presentation competition (KMICS – Hematology symposium)
• 1st prize in JAM event on National Science Day (KMICS)
• 3rd prize in inter-college PPT competition on sustainability through horticulture (Andhra Mahila Sabha College)

LANGUAGES

English, Telugu, Hindi

ADDITIONAL INFORMATION

Skills: molecular biology, tissue processing, cell culture, immunohistochemistry, genomics, chromatin accessibility assays, lab operations, inventory management, vendor coordination, DNA isolation, PCR, Western blotting

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Cover Letter:

I am writing to express my keen interest in the position of Scientist – Molecular Biology at D-NOME Biosciences, as advertised. With a Master’s degree in Animal Biology and Biotechnology and over two years of extensive hands-on experience at CSIR – Centre for Cellular and Molecular Biology (CCMB), I am confident in my ability to contribute effectively to your interdisciplinary R&D team. At CCMB, my work has focused on a range of molecular biology techniques that align closely with your requirements: from restriction digestion, ligation, bacterial transformation, plasmid isolation, and PCR to more specialized methods such as bulk ATAC-seq, CUT&RUN, and Hi-C for chromatin accessibility and genomics studies. My familiarity with protein techniques including BCA assays, SDS-PAGE, and Western blotting further complements your core focus on recombinant protein expression and purification. Additionally, my experience includes designing and troubleshooting plasmid-based constructs, optimizing expression conditions and monitoring time-course studies, maintaining and transfecting HEK293T cells as part of AAV production workflows, proficiency with data analysis tools (including R) and strong documentation practices. Beyond technical skills, I have actively supported lab operations by coordinating vendor interactions, managing inventory, and training newcomers—demonstrating organizational skills and a commitment to team success. My enthusiasm for continuous learning and passion for translational research would make me a strong fit for D-NOME Biosciences’ mission. I would welcome the opportunity to discuss how my background can contribute to your ongoing projects in recombinant protein workflows and gene editing. Thank you for considering my application. I look forward to your response. Warm regards, Dhruva Kumar Kesireddy

Introduction:

Tanushree Pathak is a dedicated researcher with a background in Biological Sciences, specializing in Cell & Molecular Biology. With a Master’s by Research from Tata Institute of Fundamental Research and a Bachelor’s from The Maharaja Sayajirao University of Baroda, Tanushree has gained valuable experience in neurobiology research focusing on mitochondrial function and neuronal communication.

EDUCATION

Master’s by Research in Biological Science (September 2021 – July 2024)
Tata Institute of Fundamental Research
Department of Biological Sciences
Grade – B+

Bachelor’s in Cell & Molecular Biology (August 2018 – August 2021)
The Maharaja Sayajirao University of Baroda
Dr. Vikram Sarabhai Institute of Cell & Molecular Biology
Grade – A

RESEARCH EXPERIENCE

Tata Institute of Fundamental Research

Cellular – Neurobiology lab
Advisor – Prof. Sandhya Koushika

Project Title: “Investigating the role of axonal mitochondria on gap junction assembly and functionality”

I have led this project to understand the importance of axonal mitochondria in modulating neuronal communication. During this research tenure, I gained extensive training experience in Molecular cloning, genetic strain development, Fluorescent Imaging, Confocal Imaging, Data analysis, and data management.

The Maharaja Sayajirao University of Baroda

Neurobiology lab
Advisor – Dr. Ravi Vijayvargia

Assisted in investigating the proteins associated with Huntingtin protein and understanding its effect on Huntington’s Disease.

HONOURS & AWARDS

• Honorary Poster Prize at the International Asia-Pacific Worm Meeting, IISc Bangalore (2024).
• TIFR-DAE funding for Master’s by Research program (2021-2024).
• Inspire Scholarship for academic excellence (2018).

SKILLS & TECHNIQUES

• Molecular Biology Techniques: Molecular cloning, PCR, DNA extraction, restriction digestion and ligations, bacterial culture and transformation, and gel electrophoresis.
• Cell Culture: Cell passaging, cell analysis.
• Genetics & Imaging: Genetic crosses, strain development, calcium imaging using confocal spinning disk, fluorescence microscopy, microinjections.
• Bioinformatics & Tools: Python, Machine learning, NCBI BLAST, Primer3, SnapGene, ImageJ.
• Data Analysis & Software: Origin, Microsoft Office (Word, Excel, PowerPoint).

CONFERENCES & PRESENTATIONS

• The Role of Mitochondrial Calcium Uptake in Touch Sensory Behavior – Asia-Pacific Worm Meeting, IISc Bangalore (2024) [Poster Presentation, Honorary Prize].
• Investigating Mitochondria-Mediated Actin Dynamics in Gap Junction Assembly – No-Garland Neuroscience Meeting, IISER Pune (2023) [Poster Presentation].
• Attended the 3rd Indian C. elegans Meeting, Trivandrum, Kerala (2022).

COURSES AND WORKSHOPS

• Certificate course in Cancer genomics (Biotechnika) – May 2025
• Certificate course in Advance Pharmacovigilance (Biotechnika) – February 2025
• Certificate course in Recombinant DNA Technology (Uka Tarsadia University, Bardoli).
• Workshop on Electron Microscopy, Fluorescent Imaging, and Protein Docking (Ahmedabad University).

COMMUNITY SERVICES & TEACHING

• Active member of Outreach Activities at the Tata Institute of Fundamental Research, engaging local residents in scientific discussions.
• Mentored a summer intern and a Master’s student during research tenure at TIFR.
• Assisted primary school students with studies.

Keywords: Molecular Biology, Cell Culture, Genetics, Imaging, Bioinformatics, Data Analysis, Cancer Genomics, Pharmacovigilance.

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Cover Letter:

I have extensive experience in molecular Cloning and technical details.

Ashwini Abhimanyu Patil

Molecular Biologist (Lab Technologist)

Objective:
I am a self-motivated, capable, and hard-working professional seeking a challenging career in a progressive environment that values excellence. I aim to leverage my skills and knowledge to contribute meaningfully to the organization’s growth and success.

Educational Qualification:

• Master of Science (Biotechnology) (2016-2018) – RTM Nagpur University, Nagpur, Maharashtra
• Bachelor of Science (Life Science-CBZ) (2013-2016) – RTM Nagpur University, Nagpur Maharashtra
• Higher Secondary School (2013) – Maharashtra State Board, Nagpur
• Secondary High School (2011) – Maharashtra State Board

Working Experience Details:

Anacon Laboratories Pvt Ltd (Sept 2024 to April 2025)

1. Technical discussion with the client regarding the Inquiry for a sample test
2. Proposal preparation against the Inquiry to onboard the client
3. Prepare and maintain the Excel report to manage the data against the Inquiry

Stemz Onco India Pvt Ltd. (Sept-2022 to July-2023)

1. Implement and Maintain standard Policies and guidelines at the Molecular Testing Laboratory
2. Calibration of Molecular Diagnostic Laboratory Instruments and Maintaining the Calibration Documents
3. Daily Instrument Maintenance and Quality Control
4. NABL Documentation

ICMR- NICPR, Noida (Dec-2020 to April-2022)

1. Skilled in Standard Laboratory Practices including Sample collection, Sample analysis, and Result uploading on the portal
2. Interpretation of data and troubleshooting
3. Procurement of kits, Consumables, Vendor Management
4. Preparing and managing all the documents related to Audit

WNS Global Services (April-2019 to April-2020)

1. Worked as Associate for International BPO at WNS Global Services

CSIR- NEERI, Nagpur (Sept 2017 to April 2018)

Project Work:

• Project Title: Food Waste Leachate Treatment with Sequence Batch Biological Granular Reactor (SBBGR)
• The aim of this study is to Develop of food waste leachate treatment method with advanced biological techniques at the lab scale
• SBBGR reactor consists of both Aerobic and Anaerobic Condition
• Physical, Chemical, and Biological parameters testing with a fix interval of time
• SBBGR shows effective reduction of COD up to 93%

Technical Skills:

• Molecular Diagnostic Lab Techniques
• RNA purification
• qPCR and RT-PCR analysis
• Instrumentation Knowledge
• Lab-scale Reactor Operation
• ELISA and Sequencing Techniques

Symposium Attended:

• Workshop on “Laboratory Diagnosis of SARS CoV2”
• National Conference on Wet Land Biodiversity Conservation

Personal Dossier:

• Date of Birth: 7th April, 1996
• Permanent Resident Address: Plot No. 17 Warhadkar Layout, Hajari-Pahad, Nagpur, Maharashtra-440007
• Marital Status: Single
• Nationality: Indian

Declaration:
I hereby declare that the information furnished above is true to the best of my knowledge.

Skills: Molecular Diagnostics, RNA Purification, qPCR, RT-PCR, Instrumentation, Lab-scale Reactors, ELISA, Sequencing

Date:

Place: Nagpur, Maharashtra (Ashwini Patil)

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Cover Letter:

Ms. Ashwini Abhimanyu Patil
Address- Plot NO. 17 Warhadkar layout Hajaripahad – Nagpur-Maharashtra -440013

Dear Hiring Team, I am writing to express my interest in the position mentioned in the published advertisement. With a diverse background in technical support, laboratory management, and molecular diagnostics, I am confident in my ability to contribute to your team. My extensive experience across various roles, including as a Technical Support Executive, Executive Laboratory Technician, and Research Associate, has equipped me with a strong skill set that aligns with the needs of your organization. Currently, as a Technical Support Executive at Anacon Laboratories-Nagpur (since September 2024), I manage troubleshooting, troubleshooting laboratory instrumentation, and providing technical assistance to clients. I have honed my ability to maintain and optimize laboratory systems, ensuring smooth operations for scientific research and diagnostics. My previous role as an Executive Laboratory Technician at Stemz Onco India Pvt Ltd (Sept 2022 – July 2023) involved assisting in cutting-edge molecular diagnostic research, and I played an integral part in the processing and analysis of clinical samples. Additionally, my experience at ICMR-NICPR, Noida (Dec 2020 – April 2022) as a Technical Support IV further enhanced my expertise in providing technical solutions within a research and diagnostic setting, specifically working with molecular biology techniques. This role allowed me to collaborate with multidisciplinary teams and troubleshoot advanced laboratory instruments such as RT-PCR machines and sequencing platforms. Earlier in my career, I also worked as an Associate at WNS Global Services (April 2019 – April 2020), where I gained exposure to client-facing roles and improved my problem-solving and customer support skills. Furthermore, my time as a Trainee at CSIR-NEERI, Nagpur (Sept 2017 – April 2018) laid the foundation for my interest in laboratory techniques, environmental research, and industrial testing. Throughout my career, I have developed expertise in a range of laboratory techniques, including nucleic acid extraction, PCR, HPLC, chromatography, and industrial wastewater analysis. I am confident that my technical knowledge, combined with my ability to adapt and provide support in fast-paced environments, would make me an asset to your team. I am particularly excited about the advertised project because of your reputation for such an exciting opportunity. I am eager to contribute to your continued success and bring my knowledge and skills to a team dedicated to excellence in the field. Your commitment to the project aligns with my passion for advancing scientific knowledge and improving health outcomes. I believe my technical expertise, attention to detail, and problem-solving skills make me a strong candidate for this position. I would be thrilled to discuss how my background and skills can contribute to the success of your team. Thank you for considering my application. I look forward to the opportunity to discuss how my experience and skills align with the needs of your team.
Sincerely,
Ms. Ashwini Abhimanyu Patil

Introduction:

Udaykanth Suryavanshi is a dedicated biochemist currently pursuing a Ph.D. in Biochemistry at the National Institute of Nutrition in Hyderabad, India. With a strong background in biotechnology and bioinformatics, Udaykanth has conducted cutting-edge research focused on the impact of vitamin B12 supplementation on neurodegeneration in rodent models of diabetes. His expertise lies in molecular biology, animal experimentation, and bioinformatics.

Education:

Ph.D. in Biochemistry (Sep 2019 – Pursuing)

Department of Biochemistry, National Institute of Nutrition, Tarnaka, Hyderabad, India
Thesis: “Impact of Vitamin B12 Supplementation on Neurodegeneration in Rodent Models of Diabetes”

M.Sc. in Biotechnology (July 2016 – May 2018)

Department of Biotechnology, University College of Science Saifabad, Telangana India
Thesis: “Role of a Functional -735 C>T Polymorphism in the promoter region of the MMP-2 gene in breast cancer”

PG Diploma in Bioinformatics (September 2015 – June 2016)

Osmania University, Telangana, India
Thesis: “3D-QSAR and Molecular Docking study of Mycobacterium Tuberculosis H37RV inhibitors by CoMFA and CoMSIA methods”

B.Sc. in Biochemistry (July 2012 – May 2015)

Osmania University, Telangana, India

Skills:

Animal Experimentation, Rodent Behavioral Studies, Cell Culture, Molecular Biology, Histology, Microscopy, Biochemical Techniques, Bioinformatics, Software Handling

Publications:

• Vitamin B12 Modulates D-Galactose-Induced Renal Dysfunction
• Vitamin B12 supplementation attenuates endoplasmic reticulum stress and neuronal cell death in the cerebral cortex of high-fat high-sucrose-fed male mice
• Neuroprotective role of vitamin B12 in streptozotocin-induced type-1 diabetic rat
• Amelioration of D-Galactose induced Renal Injury by Functional Food Mix through AGE/RAGE/NF-κB Dependent Mechanism in a Rat Model
• Effect of Sorbitol on Alpha-Crystallin Structure and Function
• In silico Modelling and Docking Studies of Camptothecin Derivatives

Talks and Presentations:

• Vitamin B12 supplementation attenuates endoplasmic reticulum stress and neuronal cell death induced by high-fat, high-sucrose diet
• Impact of vitamin B12 supplementation on streptozotocin-induced diabetic rat brain
• Role of MMP2 -735 C
• Emerging trends in mutation detection for screening of genetic disorders

Scholarships and Awards:

• DBT travel grant to attend International Conference: 22nd IUNS-ICN in Tokyo, Japan (2022)
• Qualified Telangana State-State eligibility test for lectureship TS-SET Lectureship (2019)
• Indian Council of Medical Research – Junior Research Fellowship award in Life Science Category (2018)
• Received Certificate of Appreciation as a valued Evaluator for National Camp Examination of Vidyarthi Vigyan Manthan (2019)

Workshops and Conferences Attended:

• Ad-hoc training course on “Care, Breeding, Management, Experimentation techniques and Ethical treatment of laboratory animals”
• Participated in the workshop on “Research Methodology and Skills Sets”
• Value added course on “Python programming language”
• Attended a workshop on “Purification and Biochemical Characterization of Recombinant Protein”
• Workshop on Basic of Bioinformatics
• Attended workshop on “BMC formation and PBR preparation for conservation of Biodiversity in Telangana State”
• Work carried out and presented a complete thesis on “Screening and Diagnosis of Thalassemia”
• Attended Workshop on “Radio Chemistry and Applications of Radio Isotopes”
• Participated in an International Congress of Cell Biology
• Participated in the Nano3Bio dissemination event entitled “The Future of Chitosans”

References:

Dr. G Bhanuprakash Reddy, Scientist ‘G’, Head, Dept. of Biochemistry, National Institute of Nutrition, Tarnaka, Hyderabad, India

Dr. V Sudhakar Reddy, Scientist ‘C’, Dept. of Biochemistry, National Institute of Nutrition, Tarnaka, Hyderabad, India

Dr. R Tamizhselvi, Professor Grade I, Dept. of Biotechnology, Vellore Campus, Vellore

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Cover Letter:

I am S. Udaykanth, submitted my Ph.D. thesis to Osmania University, Hyderabad in the field of Biochemistry. Over the course of my academic journey, I have gained substantial hands-on exprience in various molecular biology techniques involving RNA, DNA and protein analysis.
During my masters degree (Biotechnology) I worked with human blood samples, successfully extracting DNA and performing restriction enzyme digestion to identify the genotypes associated with breast cancer pathogenesis.
In my Ph.D work I have performed protein quantification, western blotting, RT-PCR, and ELISA techniques. I have exprience in gel filtration chromatograpghy required for protein seperation and isolation from eye lens. I have strong basis in data analysis using graphpad prism.
With my strong technical skills and laboratory experience, I beleive that I can significantly contribute in the advertised project. Please find my CV attached for ypur kind conideration. I hope to hear a positive reponse.
Thank You

Udaykanth