AMRUTHA MOHAN
CONTACT
India
PROFILE
Dedicated biotechnology professional with over 6 years of experience in research in cancer biology. Proficient in experimental design, data analysis, and laboratory techniques with the ability to collaborate with multi-disciplinary teams. Passionate about harnessing scientific exploration to address real-world challenges and drive progress in biotechnology.
EDUCATION
PhD. in Biotechnology
Manipal Academy of Higher Education (MAHE)
2017-2023
MSc. in Biotechnology
Rajiv Gandhi Centre for Biotechnology/ MAHE
PhD Student
RESEARCH EXPERIENCE
I completed my thesis work entitled “Evaluation of TIF1γ as a mediator of self-renewal of cancer stem cells in Oral Squamous Cell Carcinoma” in 6 years. Here, I got experienced in cell culture techniques and basic molecular biology and biochemical techniques. I have enough practice in FACS, Confocal microscopic staining, IHC, different electrophoresis techniques, and immunoprecipitation. I can independently design and execute experiments to address a research problem and analyze the data derived from it to reach a conclusion. In addition, I can also troubleshoot the mistakes made during the execution of the experiment to efficiently repeat the protocol by correcting the mistake. (2017-2023)
PROJECT EXPERIENCE
Project Trainee
Amrita School of Biotechnology, AmritaVishwaVidyapeetham (2014-2016)
BSc. in Microbiology
Amrita School of Biotechnology, AmritaVishwaVidyapeetham (2011-2014)
SKILLS
Cell culture, Primary cell culture, Western blotting, Immunohistochemistry, Immunocytochemistry, FACS, Basic biostatistical analysis and good mathematical skills, Ability to work in a group, Good communication skills
SCHOLARSHIPS
Qualified UGC-JRF fellowship with 71st rank in CSIR-UGC-NET examination in June 2016
Qualified CSIR-UGC NET examination for Lectureship (LS) in December 2015 with 31st rank
Scholarship for Undergraduate University Rank Holders by University Grants Commission
Amritavidhyanidhi scholarship by Amrita School of Biotechnology for top scorers in MSc entrance examination
PUBLICATIONS
1. A novel reporter construct for screening small molecule inhibitors that specifically target self-renewed cancer cells, Experimental Cell Research, 2019, IF-3.9
2. Markers and Reporters to Reveal the Hierarchy in Heterogeneous Cancer Stem Cells, Frontiers in Cell and Developmental Biology, 2021, IF-6.6
3. Reporters of cancer stem cells as a tool for drug discovery, Frontiers in Oncology, 2021, IF-6.2
4. SSTP1, a host defense peptide, exploits the immunomodulatory IL6 pathway to induce apoptosis in cancer cells, Frontiers in Immunology, 2021, IF-7.5
5. β-tubulin isotype, TUBB4B, regulates the maintenance of cancer stem cells, Frontiers in Oncology, 2021, IF-6.2
CONFERENCE PROCEEDINGS
– Poster presentation on “A Pro-tumorigenic Role of TIF1γ in Oral cancer” at 39th Annual Conference of IACR in 2020 in RGCB, Thiruvananthapuram
– Oral presentation on “TIF1γ regulates self-renewal of cancer stem cells in oral cancer” at 3rd National Biomedical Research Competition by Society of Young Biomedical Scientists, India in 2021
– Poster presentation on “TGF-β mediated induction of self-renewal: Role of Hippo and Integrin signaling pathways” at 7th International Conference on Translational Cancer Research by IACR in 2018 in IIT, Chennai
– Poster presentation on “Chemically modified Biopolymers: Synthesis and Applications” at Vidyut 2014 held at Amritavishwavidyapeetham, Amritapuri campus
– Student Volunteer at the International Biotechnology Conference BIOQUEST 2013 organized by Amrita School of Biotechnology held at Amritapuri Campus
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Cover Letter:
I am a passionate research professional with PhD degree with more than 6 years of experience. I had hands on experience in all the molecular biology and cell biology techniques. I am well experienced in identifying a research problem, planning the experiments well ahead and executing them in time, analyzing the data and troubleshooting the unexpected outcomes. My PhD work involved working with different cancer models in vitro and in vivo. It included cancer cell lines, primary cells and xenograft mice models. I have hands-on experience in various assays like FACS, confocal microscopy – both immunohistochemistry and immunocytochemistry, real-time PCR, western blotting and many other self-renewal assays as my work was on cancer stem cells. I have generated a self-renewal reporter construct through cloning, which helped my team to screen the cancer stem cells effectively. I am very much familiar with RT-PCR and qRT-PCR also, which helped in screening the expression of different self renewal genes. I believe my practical knowledge and expertise is competent enough to contribute to the role vacant in your company.
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