AMIT LADHA
Molecular Biotechnologist
[hidden] Molecular Biotechnology
University of Birmingham, UK 2022-2024
BTech in Biotechnology
NIIT University, Neemrana, India
Research Lead
Rancelab Pvt Ltd
sept’2023 to Current
Conducted WES on patient tumour samples to identify somatic mutations. Used Illumina sequencing platforms and analyzed raw data using FASTQC for quality control. Mapped sequences to the human reference genome using BWA and called variants using GATK. Identified potential driver mutations in oncogenes and tumour suppressor genes. Analyzed BRCA1/BRCA2 variants from a targeted gene panel. Used ClinVar and dbSNP to classify mutations as pathogenic, benign, or uncertain significance. STAR was used for alignment, HTSeq for gene expression quantification, and DESeq2 for differential expression analysis. Reviewed literature from PubMed, OMIM, and HGMD to validate clinical significance. Designed multiplex PCR primers for CYP450 gene variants.
[hidden] Research Student
University of Birmingham May’2023 to Sep’2023
Graduated from the University of Birmingham with a background in molecular biotechnology, specializing in investigating cancer biomarkers, designing experiments, and developing innovative approaches for therapeutic interventions.
Enhancement of immune surveillance in breast cancer by targeting hypoxic tumor endothelium: Can it be an immunological switch point? Frontiers in Oncology, 13, 1063051
The chimera of S1 and N proteins of SARS-CoV-2: Can it be a potential vaccine candidate for COVID-19? Expert Review of Vaccines, 21(8), 1071–1086
COVID-19 Pandemic Panic: Prophylactic as Well as Therapeutic Management with Traditional Ethnic Phytopharmaceuticals with Challenging Nanospray Inhaler and Advanced Drug Delivery System 2018-2022 KOLKATA, INDIA
Microsoft Office, Programming Languages: R, C, C++, Python, SQL, Machine Learning, Flutter, Bioinformatics: BLAST, GROMACS, VMD (Visual Molecular Docking), Google Workspace, Standard Operating Procedures (SOP), Knowledge of GLP and GMP, GraphPad Prism, Next-Gen Sequencing, Cell Culture, Statistical Analysis, SDS-PAGE Analysis, ELISA Assay Techniques, Western Blotting, Analytical and Preparative Centrifugation, Bacterial Culturing, Affinity Chromatography Techniques, Protein Quantification Methods, Genetic Material Amplification, High-Throughput Sequencing Analysis, Research and Collaboration, Leadership, Communication with effective Presentation Skills, Attention to Detail, Research Methodology and Design, Regulatory Affairs in Biotechnology, Time Management, Adaptability, Critical Thinking, Data Interpretation, Problem-Solving
“`
Cover Letter:
**Amit Ladha**
Kolkata, India
Date: 11/02/2025
**Hiring Manager**
MedGenome Labs Ltd.
Bangalore, India
**Subject:** Application for Genome Analyst Position
Dear Hiring Manager,
I am writing to express my keen interest in the **Genome Analyst** position at MedGenome. With a **Master’s in Molecular Biotechnology** from the **University of Birmingham** and hands-on experience in **Next-Generation Sequencing (NGS) technologies**, I am eager to contribute to your mission of advancing genomic diagnostics. My background in **variant analysis, molecular diagnostics, and bioinformatics collaboration** aligns well with the role’s requirements.
During my tenure as a **Research Lead at Rancelab Pvt Ltd**, I conducted **Whole Exome Sequencing (WES) on patient tumour samples** using **Illumina platforms**, ensuring data integrity through **FASTQC-based quality control**. I mapped sequences using **BWA** and performed variant calling with **GATK**, successfully identifying **driver mutations in oncogenes and tumour suppressor genes**. My expertise extends to **targeted gene panel sequencing**, where I analyzed **BRCA1/BRCA2 variants**, classified them using **ClinVar and dbSNP**, and assessed clinical relevance through **PubMed and HGMD literature curation**.
My ability to **collaborate across disciplines** is demonstrated by my work with **bioinformatics teams** in processing large genomic datasets. I performed **RNA-Seq analysis** using **STAR, HTSeq, and DESeq2** to identify differentially expressed genes, supporting precision medicine initiatives. Additionally, my experience in **designing molecular diagnostic assays**—including **multiplex PCR primers for CYP450 gene variants**—would be valuable for assay development at MedGenome.
Beyond technical expertise, I bring **strong analytical and communication skills**, allowing me to **interpret sequencing results, prepare clinical reports, and effectively collaborate with cross-functional teams**. My commitment to precision and efficiency is reflected in my role in optimizing **biotinylation efficiency in Turbo-ID constructs**, achieving **85% accuracy in experimental validation**.
MedGenome’s pioneering efforts in clinical genomics and large-scale projects such as **GenomeAsia 100K** resonate with my passion for cutting-edge genomic research. I am excited about the opportunity to apply my expertise in **NGS, human genetics, and molecular diagnostics** while growing within an innovative team. I look forward to discussing how my skills can contribute to MedGenome’s vision.
Thank you for your time and consideration. I have attached my **CV** for your review. I would welcome the opportunity for an interview to explore this further.
Sincerely,
**Amit Ladha**
Leave a Reply