AKANKSHA SINGH – Unleashing the Power of Molecular Biology: A Graduate’s Journey into Biotechnology

AKANKSHA SINGH

New Delhi, IN

OBJECTIVE

Result-driven graduate with knowledge of molecular techniques seeking full-time roles in Biotechnology, Molecular Biology, and its application.

EXPERIENCE

DBT Intern | Advanced Genomics Institute and Laboratory Medicine (AGILE)

JUNE 2024 – DECEMBER 2024

Gained hands-on experience in molecular biology techniques, including genome extraction from human samples and RT-PCR analysis for disease diagnosis.

Developed proficiency in laboratory protocols and data analysis for accurate disease diagnosis.

Project Trainee | ICAR- National Bureau of Plant Genetic Resources

JANUARY 2021- JUNE 2021

Genetic Diversity analysis of naked Barley (Hordeum vulgare L.) germplasm using SSRs marker

• Genomic DNA extracted manually.

• Genotyping using Thermocycler (PCR)

• Heterozygosity calculated using Genalex 6.5 software

Trainee | ICAR- National Bureau of Agriculturally Important Microorganism

MAY 2019 – JULY 2021

Molecular Characterization of U. virens that cause false in Rice

• Fungi genomic DNA extracted manually using CTAB extraction buffer (20 different fungi were used in this study).

• Genotyping using Thermocycler (PCR).

• Characterization analysis using horizontal Dendrogram.

Trainee | MRD Life Sciences- Lucknow

DECEMBER 2018 – JANUARY 2019

Basic Molecular Techniques

• DNA extraction methods.

• Introduce to various equipment of the laboratory.

• Quantification and qualification of DNA, RNA, and Proteins

EDUCATION

Masters of Technology in Biotechnology | Guru Gobind Singh Indraprastha University

2022 – 2024

GPA- 8.04/10

Bachelor of Technology in Biotechnology | Sam Higginbottom Institute of Agriculture Technology and Sciences- DU

2017 – 2021

GPA- 7.4/10

SKILLS

Molecular Techniques: DNA/RNA Extraction (CTAB method, TRIzol method, Phenol-Chloroform method, SDS extraction method), PCR (standard PCR, RT-PCR), Gel electrophoresis (agarose gel, polyacrylamide gels), Cell culture

Protein Analysis: SDS-PAGE, Protein quantification (Bradford assay, BCA assay), Protein purification

Data Analysis: Image analysis (ImageJ), Statistical analysis, Data visualization (MS Excel)

ACTIVITIES

WORKSHOP: “Using Ibin Databases for Research And Education In Ecology And Conservation” (CSIR- CIMAP) 2019 Lucknow, Uttar Pradesh, India

CONFERENCES: National Conference (Biomillap) On “Recent Advances In Agriculture, Food Tech And Human health” (Shiats-DU)- 2019 Prayagraj, Uttar Pradesh, India

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Cover Letter:

With a postgraduate degree in Biotechnology and hands-on experience in molecular biology and genomics workflows, I am passionate about contributing to impactful scientific research and discovery. During my academic journey and research training, I have worked extensively with DNA/RNA extraction methods (manual and automated), PCR and RT-PCR, electrophoresis, and molecular marker analysis. My work at various research institutes has also familiarized me with genotyping, sequence analysis tools, and basic concepts of Next-Generation Sequencing (NGS). These experiences have strengthened my technical foundation and nurtured my deep interest in lab-based research and innovation. I am particularly drawn to roles that involve method development, protocol optimization, or molecular diagnostics, where I can apply my skills and continue to grow in a collaborative and research-focused environment. I bring a disciplined work ethic, attention to detail, and an eagerness to contribute to meaningful scientific advancements.