**AKANKSHA SINGH**
New Delhi, IN
OBJECTIVE
Result-driven graduate with knowledge of molecular techniques seeking full-time roles in Biotechnology, Molecular Biology, and its application.
EXPERIENCE
DBT Intern | Advanced Genomics Institute and Laboratory Medicine (AGILE)
JUNE 2024 – DECEMBER 2024
– Gained hands-on experience in molecular biology techniques, including genome extraction from human samples and RT-PCR analysis for disease diagnosis.
– Developed proficiency in laboratory protocols and data analysis for accurate disease diagnosis.
Project Trainee | ICAR- National Bureau of Plant Genetic Resources
JANUARY 2021 – JUNE 2021
– Genetic Diversity analysis of naked Barley (Hordeum vulgare L.) germplasm using SSRs marker.
– Genomic DNA extracted manually.
– Genotyping using Thermocycler (PCR).
– Heterozygosity calculated using Genalex [hidden] software.
Trainee | ICAR- National Bureau of Agriculturally Important Microorganism
MAY 2019 – JULY 2021
– Molecular Characterization of U. virens that cause false in Rice.
– Fungi genomic DNA extracted manually using CTAB extraction buffer (20 different fungi were used in this study).
– Genotyping using Thermocycler (PCR).
– Characterization analysis using horizontal Dendrogram.
Trainee | MRD Life Sciences- Lucknow
DECEMBER 2018 – JANUARY 2019
– Basic Molecular Techniques.
– DNA extraction methods.
– Introduced to various equipment of the laboratory.
– Quantification and qualification of DNA, RNA, and Proteins.
EDUCATION
Masters of Technology in Biotechnology | Guru Gobind Singh Indraprastha University
2022 – 2024
– GPA: [hidden]
Bachelor of Technology in Biotechnology | Sam Higginbottom Institute of Agriculture Technology and Sciences- DU
2017 – 2021
– GPA: [hidden]
SKILLS
– **Molecular Techniques:** DNA/RNA Extraction (CTAB method, TRIzol method, Phenol-Chloroform method, SDS extraction method), PCR (standard PCR, RT-PCR), Gel eletrophoresis (agarose gel, polyacrylamide gels), Cell culture
– **Protein Analysis:** SDS-PAGE, Protein quantification (Bradford assay, BCA assay), Protein purification
– **Data Analysis:** Image analysis (ImageJ), Statistical analysis, Data visualization (MS Excel)
ACTIVITIES
**WORKSHOP:** “Using Ibin Databases for Research And Education In Ecology And Conservation” (CSIRCIMAP) 2019 Lucknow, Uttar Pradesh, India
**CONFERENCES:** National Conference (Biomillap) On “Recent Advances In Agriculture, Food Tech, And Human health” (Shiats-DU)- 2019 Prayagraj, Uttar Pradesh, India
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Cover Letter:
My recent internship at the Advanced Genomics Institute and Laboratory Medicine (AGILE) has equipped me experience extraction from human samples and performed RT-PCR analysis for accurate disease diagnosis. I am proficient in laboratory protocols and data analysis, ensuring reliable and timely results.
Prior to my internship at AGILE, I gained valuable experience in molecular biology techniques through various projects at esteemed institutions. At ICAR-National Bureau of Plant Genetic Resources, I delved into genetic diversity analysis of barley germplasm using SSR markers. This involved DNA extraction, genotyping, and data analysis using Genalex [hidden] software. Furthermore, my internship at ICAR-National Bureau of Agriculturally Important Microorganisms allowed me to explore molecular characterization of fungi causing false smut in rice. I gained expertise in DNA extraction, PCR-based genotyping, and phylogenetic analysis.
My initial exposure to molecular biology techniques at MRD Life Sciences further solidified my understanding of fundamental laboratory practices, including DNA extraction, quantification, and qualification. I am eager to apply my skills and knowledge to contribute to your ongoing research projects. I am particularly interested in genome sequencing, metagenomics and clinical genomics. I am a highly motivated and detail-oriented individual with excellent problem-solving and communication skills. I am confident in my ability to adapt to new challenges and work collaboratively within a team.
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