Dr. M. ANGEL NIVYA

Indian nationality

Summary

A dedicated and experienced researcher with a Ph.D. in Biotechnology and a strong background in protein purification, molecular biology, and biophysical techniques. Proficient in conducting research and publishing findings in reputable journals.

Professional Experience

Project Scientist II (March 2024 – June 2024)
Translational Health Science and Technology Institute (THSTI), Faridabad, India

Research Associate (April 2023 – February 2024)
Institute of Genomics and Integrative Biology (IGIB), New Delhi, India

Research Associate 1 (March 2019 – April 2022)
Centre for Cellular and Molecular Biology (CCMB), Hyderabad, India

Education

Ph.D. in Biotechnology, 2014 – 2018
Indian Institute of Technology Hyderabad (IITH), Hyderabad, India

M.Sc. in Biotechnology, 2009 – 2011
St. Joseph’s College, Tiruchirappalli, Tamilnadu, India

B.Sc. in Biotechnology, 2006 – 2009
T.U.K Arts College, Thanjavur, Tamilnadu, India

Skills

Protein Skills: Cloning, Expression, and Purification of Proteins, SDS-PAGE, Western blot, Bioinformatics

Molecular Biology Skills: Plasmid preparation, Transformation, PCR, DNA sequencing, RT-PCR

Biophysical Techniques: Fluorescence Polarization, Isothermal Titration Calorimetry

Computational Skills: Molecular Modelling, Docking, Simulation, Sequence Analysis

Research Experience

Peptides based Drugs: Synthesis of Peptides at Indian Institute of Science

Plant Molecular Biology: Trained at the Rajiv Gandhi Center for Biotechnology

CoVID19: Involved in Cloning and Expression of Proteins

Publications

• Tanneru N., Nivya MA., et al. (2023). Plasmodium DDI1 – International Journal for Parasitology.
• Sudhakar R., Nivya MA., et al. (2022). Antibody response in SARS-CoV-2-infected individuals – Indian Journal Medical Research.

References

Prof. Rajakumara Eerappa
Professor and HOD
Dept. of Biotechnology, IIT Hyderabad
Email: [Email Hidden]

Prof. Puran Singh Sijwali
Sr. Principal Scientist
Centre for Cellular and Molecular Biology (CCMB)
Email: [Email Hidden]

Prof. Archana Singh
Principal Scientist
Institute of Genomics and Integrative Biology (IGIB)
Email: [Email Hidden]

Prof. Milan Surjit
Professor
Translational Health science and Technology Institute (THSTI)
Email: [Email Hidden]

Honors & Awards

3rd position in 10th Class
Qualified Graduate Aptitude Test in Engineering (GATE-2014)

Work Style

Strong independent work style with excellent team work skills. Passionate and well-organized.

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Cover Letter:

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Dear Sir,
I am M. Angel Nivya, a graduate student of Prof. Rajakumara Eerappa from the Indian Institute of Technology (IIT) Hyderabad, India. I had a working experience of 3 years in the Centre for cellular and molecular biology (CCMB) under the guidance of Dr. Puran Singh Sijwali and worked as a research associate in the Institute of Genomics and Integrative Biology (IGIB) under the guidance of Dr. Archana Singh for one year. I was working as Project scientist II in the Translational Health Science and Technological Institute (THSTI) under the guidance of Dr Milan Surjit.
Research experience from Ph.D.-My expertise (in research) belongs to the cloning and purification of proteins and their biophysical and computational approaches to investigate the molecular interactions of proteins to histone peptides. Most of my Ph.D. research work focused on the UHRF1 (Ubiquitin-like, containing PHD and RING finger domain). We have demonstrated that the binding affinities of UHRF1 TTD exhibit higher binding affinity towardsH3K9me2 compared to H3K9me1/3through isothermal titration calorimetry (ITC). We have employed a computational tool, Molecular Dynamic (MD) simulation, to characterize the methyl status-specific binding of H3R2K9me by the TTD-PHD, and to analyze the effects of H3K4 di-methylation on H3R2K9me recognition. We have validated the MD simulation data through ITC binding study. These studies establish that the UHRF1 TTD-PHD exhibits lower binding free energy (ΔGbind) towards H3R2K9me2 compared to H3R2K9me3. We also showed that the SRA domain of UHRF1 binds to all 5mC oxidation derivatives.
Research Associate: In CCMB, I was working on biochemical and biophysical characterization of DNA Damage inducible (DDi1) protein from Plasmodium falciparum. Ddi1 is a retroviral aspartyl protease and is involved in the repair of DNA-protein crosslink. Ddi1 also acts as a proteasome shuttle factor. We investigated the Plasmodium DDI1 to identify its roles during parasite development and potential as a therapeutic target. We have also knocked in pfDDi 1 into Saccharomyces cerevisiae to investigate the growth effect.
In IGIB, I was working on isolation of Keratinocytes, Melanocytes and Fibroblast cells from foreskin patient samples and Cloning, Expression and purification of Transglutaminase 1(TGM1) protein from E.coli.
In THSTI, I was involved in the Development of a recombinant vaccine against Hepatitis E Virus and their immunological characterization.
Skills in Brief: I have also trained in molecular biology, biochemistry, and structural biology related techniques and also experienced in mammalian cell culture, Saccharomyces cerevisiae and Pichia pastoris. I am very well experienced in protein biochemistry, biochemical and biophysical characterization of proteins and epigenetics related concepts and experiments.
Research Interest: I am interested in DNA- protein, RNA- Protein and peptide-protein interactions, protein biochemistry, Structural biology and Epigenetics. My main goal is to setup independent research lab in good academic institute.
I believe that based on my research experience and skill set gained, I would perform to the best of ability for the given project. I am a highly motivated, innovative individual who works well independently as well as collaboratively on teams of scientists from various backgrounds. I also had experience in mentoring and training both undergraduate and postgraduate students with little prior research skills.
Thank you.
Sincerely,
Dr. M. Angel Nivya.