**Akanksha Aphale**
Summary:
Aspiring research scientist with a strong background in protein purification and molecular biology, eager to contribute expertise in R&D roles to advance cutting-edge discoveries in the biotechnology sector. Seek a growth-oriented and challenging career path to enhance organizational success through continuous learning and teamwork.
EDUCATION
2023
Master of Science in Biotechnology
Savitribai Phule Pune University
[hidden]
2021
Bachelor of Science in Biotechnology
Savitribai Phule Pune University
[hidden]
SKILLS
Protein Expression and Purification
Molecular Biology techniques
Animal cell culture
Western Blot analysis
Detail-oriented approach
Project Management
Communication
EXPERIENCE
Tata Institute of Fundamental Research, Hyderabad (TIFR)
Junior Research Fellow (One Year Project)
September 2023-September 2024
Project Title: Structural Characterization of p53 Fibrils Implicated in Human Cancers
– Structural analysis of p53 fibrils to understand their role in cancers
– Proficient in AKTA chromatography for protein purification
– Expertise in experimental design and execution
– Knowledge in molecular cloning for improved protein constructs
– Utilized DSC experiments for thermal stability analysis
Savitribai Phule Pune University, Pune
MSc. Dissertation Trainee
February 2023 – May 2023
Project Title: Determining differential gene expression regulation by p53 in colon cancer cells
– Conducted research on wild-type and mutated p53 in colon cancer cells
– Utilized ChIP-seq data analysis and qPCR for gene expression studies
– Collaborated effectively in a multidisciplinary team environment
ACCOMPLISHMENTS
– Qualified GATE 2024
– Qualified GAT B-2021 with AIR 132
– Qualified IIT JAM -2021 with AIR 798
IAS-INSA-NASI Summer Research Fellowship
Summer Research Fellow
August 2022 – October 2023
Project Title: Characterization of a Genetic Cell Line Model of Huntington’s Disease with Extended CAG Repeats
– Researched and analyzed the MTCH-1 gene in Huntington’s Disease contexts
– Applied advanced cell culture techniques and imaging methodologies
– Developed expertise in neurodegenerative disease research
– Proficient in transfection, protein analysis, and cell imaging
REFERENCE
Available upon request.
**Keywords:** Protein Expression, Protein Purification, Molecular Biology, Western Blot, Project Management, Communication, Animal Cell Culture
Cover Letter:
Akanksha Aphale
Pune-413102
18/2/25
Hiring Manager MolPath Labs
Subject: Application for Junior Research Scientist Position
Dear Sir,
I am writing to express my keen interest in the Junior Research Scientist position at MolPath Labs. With a strong background in molecular biology, protein purification, and biophysical characterization, I am excited about the opportunity to contribute to your team’s research efforts.
During my research experience, I have extensively worked on the expression, purification, and structural characterization of the p53 DNA-binding domain (DBD), including investigating its aggregation tendencies. My expertise includes working with FPLC for protein purification, selective amino acid labeling for NMR studies, and fibril generation assays under varying conditions. Additionally, I have experience in qPCR , cell culture which align with the molecular pathology approaches at MolPath Labs. My hands-on experience in biophysical techniques and solid-state NMR sample preparation has strengthened my analytical and problem-solving abilities.
I am particularly drawn to MolPath Labs due to its focus on translational research in molecular pathology. I am eager to apply my expertise to support ongoing research and contribute to advancements in biomarker identification and disease mechanisms. With my technical skills, attention to detail, and passion for scientific discovery, I am confident in my ability to add value to your team.
I would welcome the opportunity to discuss how my experience and skills align with your research objectives. Please find my resume attached for your review. Thank you for your time and consideration.
Sincerely,
Yours Truly,
Akanksha.
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