Vrushali Chaughule – Unleashing the Power of Biotechnology: Meet the Future Innovator in Regenerative Medicine

VRUSHALI CHAUGHULE

EDUCATION

ALAGAPPA UNIVERSITY, Karaikudi, Tamilnadu

June 2023

[hidden] Biotechnology

CGPA: [hidden]

University of Mumbai, Mumbai, Maharashtra

April 2020

[hidden] Biotechnology

CGPA: [hidden]

EXPERIENCE

Satyabhama Institute of Science and Technology, International Research Center

July 2023

Center for Regeneration and Studies Chennai

• One month internship cum mini-project on stem cell activation, regeneration, and wound healing studies using Eudrilus eugeniae and Perionyx excavates
• Skills Acquired: Western Blotting, BSL3, Protein purification and expression, Histology, Immunofluorescence/Immunochemistry, Immunohistology, Cell culture and related assays, animal handling

PUBLICATIONS

• Phenylpropanoid-infused nano-ointment precision healing for antimicrobial and diabetic wounds, leveraging industrially scalable components and nano-innovation for enhanced recovery Journal: The AAPS journal DOI: [hidden]
• Understanding the molecular mechanism of regeneration using Apoptosis Induced Compensatory Proliferation (AICP) in earthworms Journal: In Vitro Cellular and Developmental Biology – Animal DOI: [hidden]

DISSERTATION PROJECTS

Evaluation of nanocomposite as in vitro diabetic wound healing formulation

June 2023

Microbial Technology Dr. SKP Lab Karaikudi, Tamilnadu

• Formulated and optimized loaded metal nanoparticles
• Confirmed its antimicrobial activity of the nanoparticles against various organisms
• Characterized formulated nanoparticles using SEM, TEM, FTIR, XRD, EDX, Fluorescence Microscopy, etc.
• Evaluated the activity In vitro for wound healing and antidiabetic activity with cell lines (HepG2, HaCat), Frantz cell diffusion

Extraction and estimation of pigments from gut flora of Parastromateus niger

Feb 2020

Dr. Veena Maheshwari, Phd Andheri, Mumbai

• Cultivated the gut flora of the Parastromateus niger
• Selected and harvested the pigmented bacteria from mixed culture plates
• Isolating the pigment from organisms and analyzing it using a spectrophotometer

ACHIEVEMENTS

• Acquired Scholarship for supporting [hidden] Biotechnology
• Secured All India Rank-72 in OBC Category in GAT-B examination

WET LAB SKILLS

Molecular techniques

• Separation of biological macromolecules (Animal cell, Plant tissue and microbes)
• PCR (qPCR, multiplex PCR, colony PCR, reverse transcriptase PCR)
• Western blotting, SDS-PAGE, 2-D gel electrophoresis, Gel electrophoresis
• Immunofluorescence, Immunohistology

Microbiological techniques

• Handling, maintaining the microbes
• Disinfection and sterilization methods
• Antibiotic susceptibility testing
• Microbial assays (well-diffusion/spot/disc-diffusion assay, time-kill assay, checker-board assay, etc.)

Formulation development techniques

• Excipient compatibility studies
• Process feasibility studies
• Antibiotic susceptibility testing

Cell Culture techniques

• Handling and maintenance of cell culture
• Wound healing assay
• MTT cytotoxicity assay

In vivo techniques

• Handling and maintenance of mice, zebra fish, Eudrilus eugeniae, and Perionyx excavates
• Good laboratory practices

DRY LAB SKILLS

Bioinformatics

• Programming languages – Python and R
• Phylogenetic analysis, Genome annotation, Primer designing
• Genomic and proteomic data analysis
• Molecular docking, DGE (differential gene expression)
• Statistical data analysis and prediction through SPSS and Origin Pro
• Image processing using ImageJ

Scientific writing and Communication

CONFERENCE PARTICIPATION

• International Genomics India Conference Genotypic Technology Pvt. Ltd.
• National Conference on Biotechnology and Omics Sciences Alagappa University

SEMINAR/WEBMINAR AND WORKSHOPS ATTENDED

• Workshop NGS analysis and Genome informatics program
• Workshop Data Science and Machine Learning with – R
• Webminar Bioinformatics Webminar Series
• Webminar Computer-Aided drug design
• Symposium One day national symposium on IPR (Industry-institutional-MSME collaboration)

SOFT SKILLS

• Presentation and communication skills
• Logical reasoning and scientific temperament
• Flexible and adaptable, Problem solver
• Coordinated
• Leader and team player

Skills: Molecular techniques, Microbiological techniques, Formulation development techniques, Cell Culture techniques, In vivo techniques, Dry Lab Skills, Bioinformatics, Scientific writing and Communication

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Cover Letter:

Dear hiring manager,
Myself Vrushali Chaughule, a recent graduate with biotechnology degree. I found myself applying for this job because MedGenomes groundbreaking work in rare diseases, cancer genomics and population scale sequencing, which resonates with my career aspirations. I was impressed by your platform for cancer immunotherapy OncopeptTm and initiative Firma Genomics for rare disease diagnostics.

My aspirations to contribute into public health services motivated me throughout my studies, and I’m eager to apply my skills to your future initiatives.

I have attended workshops, seminars and webinars for NGS data analysis and genome informatics, but still keen to develop my expertise in the same and more. I have trust that combination of my technical expertise and enthusiasm for genomics makes me strong candidate for this role.

Sincerely,
Vrushali Chaughule