**Purbali Mitra**
Flat B2, Manicktala Housing Estate
Kolkata 700054, West Bengal, India
**EDUCATION**
**University of Southampton**
Southampton, United Kingdom
September 2023 – September 2024
Master of Research (MRes) in Advanced Biological Sciences; Pass
– Relevant modules: Selective toxicity, Biofilms, Evolution and genetics, Current Research
– Relevant coursework: Genomic analysis, critical evaluation, outreach activity, journal clubs
**Gandhi Institute of Technology and Management (GITAM)**
Visakhapatnam, India
July 2018 – November 2020
MSc in Microbiology, first class with distinction
– Relevant modules: Virology, Immunology, Biochemistry, Cell Biology, Molecular Biology, Medical Microbiology, Recombinant DNA technology, Fermentation Technology
– Electives: Cancer Biology, Biotechnology
**Asutosh College, University of Calcutta**
Kolkata, India
July 2015 – June 2018
BSc Honours in Microbiology; Upper second class in honours
– Relevant modules: Cell biology, Biophysics, Biostatistics, Molecular Biology, Biochemistry, Immunology, Medical Microbiology, Industrial Microbiology, Food Microbiology, Stereochemistry
– Electives: Chemistry, Botany, and Environmental Science
**Kendriya Vidyalaya, Central Board of Secondary Education (CBSE)**
Kolkata, India
March 2013 – May 2015
A-levels, Science stream with PCMB, Biology (A1), English (A1), Chemistry (A2), Physics (A2), Mathematics (B1); Percentage 84%
**Kendriya Vidyalaya, Central Board of Secondary Education (CBSE)**
Kolkata, India
March 2011- March 2013
GSCE level, Science (A1), Mathematics (A1), English (A2); CGPA [hidden]
**RESEARCH EXPERIENCES**
**Functional Microbiome Research Lab**
School of Biological Sciences, University of Southampton
Postgraduate researcher
September 2023 – September 2024
Isolation, identification, and drug susceptibility characterization of human microbiome members
– Independently completed two different projects
– Isolated and preserved many colonies from skin, gut, and oral parts; extracted DNA; amplified 16S rRNA genes using PCR; agarose gel electrophoresis for visualizing bands; purified PCR products for Sanger’s sequencing
– Analyzed gene sequences using SnapGene, FinchTV, and CodonCode Aligner; evaluated using tools like NCBI BLAST and eHOMD
**Dr. Rajkumar Banerjee Lab**
Department of Applied Biology, CSIR-IICT, India
Dissertation student
February 2020 – May 2020
Receptor-targeted lipid hybrids for cancer treatment
– Investigated the role of liposomes for target gene delivery in cancerous cell lines with glucocorticoid receptor
**Dr. Anusri Tripathi Lab**
Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata, India
Summer intern
May 2019 – July 2019
Role of gyrA genes in multidrug resistance development among pathogenic Enterobacteriaceae
– Aimed to investigate the gene mutation on multidrug resistance development in pathogenic bacteria from clinical settings
**RELEVANT EXPERIENCES / CERTIFICATIONS**
**Virtual Training**
EDUFABRICA in association with Springfest IIT Kharagpur, IIT Kharagpur, India
October 2020 – November 2020
Molecular Biology and Biochemistry Techniques
– Online certification course on molecular and biochemistry techniques
**Short-Term Training Program**
Department of Biotechnology and Medical Engineering, NIT Rourkela, India
February 2019 – February 2019
Genomic and Proteomic Analysis for Developing Biosimilars (GPP-2019)
– Hands-on training in molecular biology wet lab techniques and dry lab techniques using bioinformatics tools
**Programming Language Training**
The Institute of Biotechnological Research (TIBR), Gujarat, India
November 2024 – December 2024
Hands-on-training in R programming
**Bioinformatics winter internship**
Biotecnika, Bangalore, India
November 2024 – January 2025
Hands-on-training in global research tools and techniques
**RESEARCH SKILLS**
– Biological: Maintaining pure cultures; Anaerobic cultivation; MIC; Antibiotic susceptibility testing (AST); growth analysis; mammalian cell culture and maintenance; DNA/RNA/Plasmid isolation; PCR; agarose gel electrophoresis; genomic analysis; gene sequencing; immunoelectrophoresis; ELISA; TLC; enzymatic assays; handling pathogenic samples; cell transfection; WIDAL & VDRL; immunodiffusion
– Computer skills /data analysis: SnapGene; CodonCode Aligner; FinchTV; ChromasPro; datamonkey; Microsoft Office (Excel / Word / PowerPoint); ExPASy; R programming
– Communication skills: verbal presentations of scientific literature in journal club, written presentations, discussion with diverse peers; outreached project to lay audience; Languages: English, Hindi, Bengali
**PRESENTATIONS**
– Paper presentation: TCA and SSRI Antidepressants Exert Selection Pressure for Efflux-Dependent Antibiotic Resistance Mechanisms in Escherichia coli, Ou J et al., 2022, mBio 13: e02191-22, Functional Microbiome Research Journal Club, School of Biological Science, University of Southampton, November 2023
– Paper presentation: Cholinergic pesticides cause mushroom body neuronal inactivation in honeybees, Palmer et al., 2013, Nat Commun 4, 1634, School of Biological Science, University of Southampton, January 2024
– Paper presentation: Optimizing ex-vivo culture conditions to study the human gut microbiome, Tao X et al., 2023, ISME COMMUN. 3, 38, Functional Microbiome Research Journal Club, School of Biological Science, University of Southampton, April 2024
– Project presentation: Isolation, identification, and drug susceptibility characterisation of the human microbiome members, MRes project, Biological sciences postgraduate seminars, Microbiology Research seminars, School of Biological Sciences, University of Southampton, June 2024 and July 2024
**OTHER EXPERIENCES**
– Student Co-designer, University of Southampton, United Kingdom, November 2023 – May 2024
– Outreach exhibition, School of Biological Sciences, University of Southampton
Information booklet on gut health and antimicrobial resistance; Percentage 62%
**EXTRACURRICULARS**
– Member of the KPOP Society at the University of Southampton (2023-2024)
– Member of the Life Science postgraduate society at the University of Southampton (2023-2024)
– Member of Student Co-design Panel under Student Ambassador (2023-2024)
**Skills**
Molecular Biology, Biochemistry, Microbiology, Genomic Analysis, Data Analysis, Research Skills, Presentation Skills, Communication Skills, Biological Techniques, Bioinformatics, R Programming, Antibiotic Susceptibility Testing, Cell Culture, ELISA, PCR, Gel Electrophoresis, Language Skills.
Cover Letter:
Purbali Mitra
Bangalore, India
11 February, 2025
Hiring Manager
MedGenome Labs Ltd.
Bangalore, India
[Subject: Application for Genome Analyst Position]
Dear Hiring Manager,
I am excited to apply for the Genome Analyst position at MedGenome Labs Ltd. With a strong background in molecular biology, microbiology, and bioinformatics, I am eager to join your mission to advance genomic research and clinical diagnostics.
During my Master of Research (MRes) in Advanced Biological Sciences at the University of Southampton, I had hands-on experience in NGS data analysis, genome sequencing, and variant interpretation. My research focused on microbial genome identification and antibiotic susceptibility, where I applied Sanger sequencing, BLAST analysis, and multiple sequence alignments to detect bacterial species from human microbiomes. I also had experience in R and Python programming for bioinformatics analysis, which enabled the management of large genomic datasets.
My attention to detail, analytical skills, and ability to work with bioinformatics and laboratory teams make me well-positioned to meet this role’s demands. I am particularly excited about the possibility of working with cutting-edge genome sequencing platforms at MedGenome and assisting in variant analysis, literature curation, and molecular diagnostic assay development.
I would appreciate the opportunity to discuss how my experience and skills can benefit MedGenome’s mission. Thank you for your time and consideration.
Sincerely,
Purbali Mitra
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