SUMMARY STATEMENT

Experienced in Molecular biologist with proven experience improving the scientific community by performing accurate research while complying with laboratory safety protocol and standards. Self-initiated genetics graduate and Biotechnology (DBT Sponsored) postgraduate with a record of providing outstanding growth. A great motivator who thrives on positive results and seeks opportunities to continue learning and growing. Excellent work ethic, motivated to perform well both individually and as a team.

PROFESSIONAL EXPERIENCE

09/2024-Present Associate Scientist – Praesidia Biotherapeutics Private Limited, Bangalore

– In vivo: Pharmacokinetics studies on rats for prodrug parent drug, dosing (Intravenous & Oral), restraining animals (rats and mice), collection.
– In vitro: Cancer cell line culture, maintenance, passaging, GLP1 functional assay with HEK 293 cells, RT-PCR (mycoplasma testing), graph pad prism (data analysis).

12/2023-08/2024 Project Associate 1 – Institute for Stem Cell Science & Regenerative Medicine (InStem), Bangalore

– Cloning of SgRNA constructs in Humanized Cas9 containing expression vector, bacterial culture, Nucleic acid isolation, Competent cell preparation (DH5α), transformation, HEK293 cell culture, maintenance, freezing, thawing, transfection (Calcium phosphate), Stem cell line (HEM 20), culture, maintenance, passaging (physical cutting method), transfection (lipofection), freezing, thawing.

10/2023-12/2023 Project Fellow – L V Prasad Eye Hospital, Hyderabad

– Genomic DNA isolation, PCR, Ligation, cloning.

11/2022-01/2023 Project Associate – Nanobiotechnology Research Foundation

01/2022-06/2022 Research Project – Rama Devi Women University, Bhubaneswar

06/2021-02/2022 Internship Trainee – Indian Institute of Science Education and Research Kolkata

01/2020-03/2020 Research Project – Institute of Genetic Engineering

07/2019-08/2019 Internship Trainee

EDUCATION

NRS Medical College

2020-2022 [hidden] (DBT Sponsored) Biotechnology

Rama Devi Women University, Bhubaneswar

2017-2020 [hidden] (Genetics)

Maulana Abul Kalam Azad University (Institute of Genetic Engineering)

2015 Intermediate

Lake Town Govt. Sponsored Girl’s High School

2013 Matriculation

LANGUAGES

English, Hindi, Bengali

SKILLS

Molecular techniques, Microbiology, DNA, RNA isolation, PCR, cDNA synthesis, E. coli culture, RT-PCR, SgRNA constructs cloning, Competent cell preparation (DH5α), Plasmid isolation, Restriction digestion, ligation, antimicrobial assay, Primer designing, transformation, Bioinformatic tools, Bacterial identification, Minimum inhibitory concentration, NGS, Cell Biology, Immunology, Karyotyping, ELISA, Blood group, Computer Knowledge (MS Word, PowerPoint, Excel)

PROJECTS

01/2022-06/2022 ([hidden]) – Biochemical analysis of roasted Koraput Coffee by FTIR and antimicrobial analysis:

First, I made coffee extract using distilled water, acetone, benzoic acid, acetic acid, ethanol, methanol, etc. I used a rotary evaporator to concentrate extracts and used them to analyze antimicrobial properties by agar plate diffusion method. Concluded that Koraput coffee has more phenolic compounds than other coffees, giving it antimicrobial properties.

01/2020-03/2020 ([hidden]) – Polytene chromosomes abnormalities due to artificial food color:

Cultured Drosophila in normal food and food with food color. Counted the number of larvae, pupa, and adult Drosophila from each vial, then compared the numbers between normal culture and food color-treated. Collected salivary glands from normal and treated larvae, made slides of polytene chromosomes, and compared them. Noted that treated adult flies could not fly as high as normal flies, and treated larvae showed locomotory abnormalities compared to normal larvae.

REFERENCES

Dr. Aaveri Sengupta: Chief Scientist (InVivo Team) Praesidia Biotherapeutics, Bangalore – Contact: [Phone Hidden], Email: [hidden]

Dr. Sandeep Barodia: Chief Scientist (InVitro Team) Praesidia Biotherapeutics, Bangalore – Contact: [Phone Hidden], Email: [hidden]

DECLARATION

I hereby declare that all the above information is true to the best of my knowledge.

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Cover Letter:

Respected mam,
I am Anchita Roy currently working as an associate scientist in Preasidia Biotherapeutics which is incubated at CCAMP. I met today with Dr. Mahesh Sahare sir at Mice facility; he suggested me to talk to you. In Preasidia Biotherapeutics, I am working with the in vitro team as well as invivo team. I can join immediately if the role will be suitable for me. I am doing cell culture (mammalian cell line and cancer cell line) and established functional assay as well as NanoBRET assay. I am doing mouse and rat handling dosing organ collection [hidden] I served as a project associate in inStem Bangalore in the Stem cell facility in the project of “CRISP cas9 based reporter cell line generation”. I did cloning transformation, stem cell line culture, HEK293 cell line culture, and transfection. I have done my DBT Sponsored master’s in the field of Biotechnology from the RAMA DEVI WOMEN’s UNIVERSITY, Odisha in 2022. I have gone through the company profile and found that research areas are well matched to my interests. I can also do work on any topic but I need your guidance and supervision. I know techniques regarding mammalian cell culture and analysis and embryonic stem cell culture also, I am really wanting to join your company as a junior scientist. Then if you think that I am deserving, then I will be able to climb ladder there, please give me an opportunity to explore your company research area. I wanted to meet with you in person and if possibilities are there, I will love to give an interview in person cause I am here in Bangalore doing a job in inStem now. Thank you very much for your time and consideration. Here I have attached my CV for your reference.
Yours sincerely,
Anchita Roy