ABOUT ME
A passionate biotechnology researcher with experience of 1 year 7 months in organizations like CSIR-CSMCRI, Bhavnagar, and Genesys biologics, Hyderabad. Currently working in R&D Process development lab of Genesys biologics with a focus on performing upstream process development activities and molecular biology techniques for cell line development for Insulin Glargine and Aspart.
WORK EXPERIENCE
Trainee – R&D Upstream Process Development and Molecular Biology Techniques
GeneSys Biologics: November 2023 – Present
- Upstream Process Development for Insulin Lispro and Glargine
- Seed media, fermenter media, and fermenter feeds preparation
- Fermenter batch handling with mixed feeding and separate feeding
- CIP, FSIP, ESIP of fermenter
- Isolation of inclusion bodies
- Buffer preparation
- Cell lysis
- Solubilization and Refolding of Insulin Proglargine
- DOEs of Solubilization and refolding of Insulin Proglargine
- Shake flasks experiments
- Microscopy
- Gram’s staining
- Preparing Batch processing record of process
- Preparing Process development report of process
- UV/Vis Spectroscopy
- Centrifugation
- Molecular Biology Techniques
- Transformation of recombinant plasmid into cell
- LB media preparation
- Cell bank preparation
- Hands-on in Thermomixer
- Spread plate and pour plate
Project Intern – Applied Phycology and Biotechnology Division
CSIR- Central Salt and Marine Chemicals Research Institute: January 2023 – June 2023
- Thermo-chemical conversion and kinetic evaluation of Dunaliella sp. Biomass to biochar and its utilization in remediating naphthalene from wastewater
- Microalgae sample collection
- Zarrouk’s media preparation
- Cultivation and harvesting of biomass
- Extraction and estimation Protein, lipid and carbohydrate
- Thermogravimetric of analysis of the biomass
- Batch absorption of naphthalene onto biochar
- Sample preparation for FTIR
- Sample preparation for SEM
- Technical report preparation
EDUCATION
[hidden] in Biotechnology ([hidden] CGPA)
Vellore Institute of Technology [2021 – 2023], Address: Vellore, Tamil Nadu (India)
[hidden] in Botany, Chemistry, and Zoology ([hidden] %)
Jaipur National University [2018 – 2021], Address: Jaipur, Rajasthan (India)
PUBLICATIONS
- Thermo-chemical behavior of Dunaliella salina biomass and valorizing their biochar for naphthalene removal from aqueous rural environmental (under the supervision of Dr. Dinesh kumar Ramalingam, Senior Scientist, CSIR-CSMCRI)
Chemosphere [2024]
- Autophagy and Mitophagy as potential therapeutic targets in diabetic heart condition: Harnessing the power the Nanotheranostics
Asian Journal of Pharmaceutical Sciences [2024]
- Nanomaterials-assisted photothermal therapy for breast cancer: State-of-the-art advances and future perspective
Photodiagnosis and Photothermal Therapy [2024]
LANGUAGE SKILLS
- Mother tongue(s): Hindi
- Other language(s): English
SKILLS
Hands-on experience in 20 L Bioreactor, PANDA Homogenizer, 500 mL, 20 L, and 100 L refolding fermenter vessel, LAFU, shaker incubator. Proficient in Microsoft Word, Excel, PowerPoint, and Teams.
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Cover Letter:
A passionate biotechnology researcher with experience of 1 year 7 months in different organizations like CSIR-CSMCRI, Bhavnagar and Genesys biologics, Hyderabad. I have been associated with project- Thermochemical conversion and kinetic evaluation of Dunaliella sp. biomass into biochar and its utilization in remediating naphthalene in CSIR Bhavnagar under the guidance of Dr. Dinesh kumar Ramalingam and Dr. Anil kumar Madhava, Senior Scientists in CSIR-CSMCRI, Bhavnagar. Currently working in R&D Process development lab of Genesys biologics to perform different upstream process development activities and molecular biology techniques for cell line development for Insulin Glargine and Aspart.
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