Umera J. Patwekar
An adept microbiologist with 18+ years of experience in Microbiology, Molecular biology, Biochemistry, and Serology. Looking forward to seeking an active role in a dynamic and growth-oriented organization, where I can contribute my combined knowledge and experience to develop innovative products and processes.
Work History
Technician
National Centre for Microbial Resources, Pune
2017-04 – Current
- DNA/RNA extraction, PCR, Sanger sequencing, curating, and data analysis.
- Involved in paid service of identification by 16S rRNA gene sequencing.
- Performing NGS Illumina Miseq.
- Microbial taxonomy and characterization using various physiological and chemotaxonomic approaches.
Technician
Microbial Culture Collection, Pune
2009-07 – 2017-03
- Isolation, characterization, maintenance, preservation, passage, and supply of microbial cultures.
- Lyophilisation of bacterial cultures.
- Handling more than 25000 bacterial and fungal isolates.
Technician
National Institute of Virology, Pune
2008-01 – 2009-07
- Cell line maintenance, performing ELISA, PCR, Gel electrophoresis.
- Worked on Japanese encephalitis and West Nile viruses.
Laboratory Incharge
Unicare Pvt Ltd, Solapur
2007-01 – 2008-01
- Worked in IVF and ICSI ART procedures.
- Assisted families in navigating complex healthcare systems.
Education
[hidden]: Microbiology
SRTM University – Nanded
GPA: [hidden]
2003-01 – 2005-04
Bachelor of Science: Microbiology
Shivaji University – Kolhapur
GPA: 70%
2000-01 – 2003-04
Diploma in Medical Laboratory Technology
Jijamata Vidypeth – Mumbai
GPA: 64
1999-01 – 2000-04
Skills
- Molecular biology and sequencing
- DNA isolation
- PCR
- Sanger sequencing
- NGS Illumina bioinformatics analysis
- Microbial taxonomy characterization
- 16S rRNA gene-based identification
- MALDI TOF-MS
- FAME
- Polar lipid profile
- Antibiotic sensitivity assays
- Biochemical characterization
- Microscopy and Scanning electron microscopy
- Isolation, maintenance, and preservation of microbial cultures
- Lyophilisation
- Handling antimicrobial resistance bacteria
- Phlebotomy
- Analysis of clinical samples
- Histopathology and Cytology
- Training Instruments
- Monitoring of Dissertation Students
- Scientific record keeping
- Preparing SOPs
- Project Management
- Analytical and problem-solving skills
- Motivates and inspires the team
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Cover Letter:
Dear Hiring Manager,
I am writing to express my keen interest in the position of junior Analyst available at Medgenome. I came to know the vacancy from biotecnika, to introduce my self, I am Umera Patwekar completing my Master degree in Microbiology and working from the esteemed NCMR National Centre for Cell Science, where I currently serve as a Research Technician, I have acquired 18 years of specialized experience in Molecular biology, sequencing techniques, particularly Sanger sequencing and NGS along with Microbiology technics.
Throughout my academic and professional journey, I have excelled in molecular biology, specializing in sequencing methodologies and taxonomically identification of microbes by different approaches within the microbiology domain. My expertise encompasses NGS, Sanger sequencing and investigating pathogenicity by screening antimicrobial compund. With 6 publications to my credit, my track record includes conducting independent research and demonstrating a strong commitment to rigorous scientific inquiry and knowledge dissemination through publishing in reputable scientific journals. Additionally, I possess proficiency in taxonomically identification of microbes by physiological, biochemical (API, BIOLOG, VITEK), Chemotaxonomy ( MALDI TOF, FAME analysis, Polar lipid and quinone.) and by molecular approach 16S rRNA gene sequencing furthermore, I have actively mentored students, trainees and technicians, contributing to their growth and proficiency in the field.
During my tenure at the National Centre for Cell Science, Previously MCC now NCMR, as belong to be a part of a microbial culture collection individually handling, maintaining, supplying and keeping all the data record of 25000 pure bacterial cultures for long term cryopreservation and lyophilisation and passage the same. Along with these I m handling Antimicrobial resistance repository. and actively involve service of Sanger sequencing, Scanning Electron Microscopy and MALDI- TOF Analysis, furthermore I had spearheaded the development of Standardized of polar lipid technique resulting it use for as service providing by NCMR NCCS which is useful for project growth potential. I was actively involved in pioneering research initiatives, including the Isolation, characterisation,identification and screening of Antimicrobial compound. These endeavors showcased my proficiency in microbial taxonomy and next generation Illumina sequencing techniques. Furthermore to contribute in drug discovery now pursuing a course of AI ML in Biology, Bioinformatics & Computational Biology Training Program by BioTenika, working on Natural product drug discovery : Applying machine learning to discover novel drug from natural product, plants and microorganism.
Thank you for considering my application. I am looking forward to the opportunity to discuss how my background and expertise align with the needs of your team. Please find my resume attached for your review.
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