Pooja Tambe
Summary: Highly motivated MSc Biotechnology graduate with a solid foundation in microbiology and immunological techniques. Experienced in green synthesis, extraction, and anticancer property evaluation through dissertation research on saffron Power and MDAMB-231 cell line. Proficient in molecular biology techniques, including plasmid and RNA/DNA isolation. Skilled in bioinformatics tools such as SwissADME, Autodock, and Pyrex. Adept at troubleshooting and passionate about contributing to innovative research and development projects. Seeking a challenging position to apply expertise and drive advancements in the biotechnology field.
Personal Information
- Nationality: Indian
- Religion: Hinduism
- Gender: Female
- Place: Indore
- Date of Birth: June 24, 2001
- Marital Status: Single
Education
- Government Holkar Science College
- BSc Biotechnology – [hidden]
- Dy Patil School of Biotechnology and Bioinformatics
- MSc Biotechnology – [hidden]
Experience
Lifesenz Cancer Research Labs Pvt Ltd
- Dissertation Project Trainee
- Conducted comprehensive research on cancer cell lines, including maintenance and media preparation.
- Investigated the anti-cancer and antioxidant properties of saffron (Crocus sativus) in breast cancer cell lines.
- Performed advanced laboratory techniques including High-Performance Liquid Chromatography (HPLC), Bradford assay for protein estimation, plasmid isolation, and DNA/RNA isolation.
- Utilized agarose gel electrophoresis for nucleic acid analysis and qPCR for gene expression studies.
- Employed lyophilization for sample preservation and analyzed the effects of saffron combined with other drugs such as vanillin and atropine on cancer cell lines.
Conference
Embrace Zebrafish
- Gained in-depth knowledge on the use of zebrafish as a suitable model for biomedical trials, with 80% genetic similarity to the human genome, enhancing its relevance for genetic and disease research.
- Observed and learned about zebrafish feeding and breeding techniques, essential for maintaining healthy experimental populations in research settings.
Skills
- Western Blotting
- Agarose gel electrophoresis
- HPLC
- Mammalian Cell Culture
- Blood Group Analysis
- Molecular biology skills
- Bioinformatics skills
- Troubleshooting
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Cover Letter:
Subject: Application for Genome Analyst Position
Respected sir,
I am excited to apply for the Genome Analyst position at Medgenome. With a Master’s degree in Biotechnology (CGPA: [hidden]) and hands-on experience in molecular biology techniques, bioinformatics tools, and analytical assays, I am eager to contribute my expertise to your team.
During my dissertation, I investigated the anticancer and antioxidant properties of Crocus sativus flowers in cancer cell lines. This research involved green synthesized extraction techniques with assays involved such as MTT, ROS, colony forming assay, etc. I have experience in DNA/RNA isolation, protein estimation assays, analytical skills such as HPLC and qPCR, and computational tools like PyRx, SwissADME, and AutoDock for molecular docking and drug discovery.
My proficiency in both wet lab techniques and bioinformatics equips me to analyze genomic data effectively. I am highly motivated to apply my skills in genomics and data interpretation to advance research and innovation at Medgenome. I welcome the opportunity to discuss how my background aligns with your needs.
Thank you for your time and consideration. I look forward to your response.
Best regards,
Pooja Tambe
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