Ragavan P – Unlocking the Secrets of Biotechnology: A Journey Through Molecular Mastery!

RAGAVAN P
BIOTECHNOLOGIST

SUMMARY
Motivated biotechnologist with hands-on experience in molecular biology, plant tissue culture, gene cloning,
rDNA technology, molecular marker analysis, microbiology, genetic transformation, and bioinformatics
tools. Passionate about advancing research in biotechnology and contributing to cutting-edge solutions in the
field.

EDUCATION
B. Tech Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu

EXPERIENCE
INTERN
Bio seed Research India (a division of DCM Shriram Ltd.), ICRISAT, Telangana (March 2024 – May 2024)

• Conducted molecular biology experiments for the isolation, cloning, and characterization of the cry2 gene from native Bacillus thuringiensis, contributing to research on biopesticides
• Performed DNA extraction, PCR amplification, and agarose gel electrophoresis, ensuring accuracy in gene analysis and downstream applications
• Ligation of the cry2 gene into plasmid vectors and transformation into E. coli (host) cells for gene expression studies, improving the cloning efficiency by 20%
• Analyzed gene expression using IPTG induction and purified recombinant proteins using SDS-PAGE, achieving high-purity protein samples suitable for further studies

Skills Gained at Bioseed Research India:

• Molecular Biology: DNA/RNA isolation, PCR, gene cloning, and gene expression (IPTG induction)
• Protein Analysis: SDS-PAGE, protein isolation, and purification
• Microbiology: Media preparation, sterilization, and microbiological techniques
• Bioinformatics: SnapGene (DNA visualization, primer design, cloning simulations)
• Electrophoresis: Agarose gel electrophoresis, SDS-PAGE

PROJECT
Exploring the Antimicrobial and Food Preservative Properties of Crescentia cujete (Calabash Fruit)

• Conducted extraction and identification of active compounds using GC-MS technique
• Screened Crescentia cujete fruit extracts for phytochemicals
• Investigated antimicrobial, antioxidant, and anti-inflammatory properties and prepared a detailed report on findings

SKILLS AND TECHNIQUES

WET LAB SKILLS

• Molecular Biology: DNA/RNA extraction, PCR, gel electrophoresis, sequencing analysis, plasmid extraction, and competent cell preparation
• Protein & Immunological Techniques: ELISA, recombinant protein expression (bacterial vector), protein purification, SDS-PAGE, antimicrobial testing
• Plant Biotechnology: Agrobacterium-mediated genetic transformation (Tobacco, Tomato), marker-assisted selection
• Plant Tissue Culture: Micropropagation (Banana, Sugarcane, Rose)
• Microbiology: Microbe isolation, plating methods, wine fermentation
• Lab Expertise: Data documentation, troubleshooting experimental protocols

DRY LAB SKILLS

• SnapGene, Mapmaker, WinQTL, NTSYS, TASSEL, BLAST & variants, MEGA Software (Phylogeny tree construction), Homology modeling (SWISS-MODEL), RasMol (Protein and nucleic acid visualization), Sequence submission tool (BankIt, Sequin)

INSTRUMENTS HANDLED

• Molecular Biology & Microbiology: Thermocycler (PCR), centrifuge, gel documentation, microscope
• Protein & Electrophoresis Techniques: SDS-PAGE, gel documentation
• Sterile Techniques: Laminar airflow, pipette handling
• Analytical Instruments: Spectrophotometer, pH meter

Soft skills

• Proficient in Microsoft Word, Excel, PowerPoint
• Strong time management, teamwork, and organizational skills
• Demonstrated leadership in group settings and problem-solving capabilities

CERTIFICATIONS

• Entrepreneurship Development in Commercial Tissue Culture – ICAR
• SWAYAM Online Course – Food Safety and Quality Control
• National Service Scheme Volunteer – Tamil Nadu Agricultural University
• Organizer of National-Level Technical Symposium “Bioconcorrenza 2023”

Keywords: Molecular Biology, Plant Tissue Culture, Gene Cloning, rDNA Technology, Bioinformatics, Microbiology, Genetic Transformation, Protein Analysis, Plant Biotechnology, Wet Lab Skills, Dry Lab Skills, Soft Skills, Certifications
“`

Cover Letter:

I am B.Tech biotechnology graduate with hands on experience in molecular biology and bioinformatics. I believe my background skills and experience has suitable for the applied post. I am looking forward to work with you. Thank you