Sharvari Nitin Paranjape

About

I have completed my [hidden] Biotechnology from Sinhgad College of Engineering. I am a hardworking student looking forward to learn and understand new concepts from a providing organization. Also, to utilize my knowledge to achieve desirable results for achieving organization goals.

Skills

• Molecular biology techniques: PCR, RT-qPCR, Gel Electrophoresis
• Quantification Techniques: Gas Chromatography, TLC, HPLC, SDS-PAGE, Tangential flow filtration
• DNA/RNA isolation
• Microbiology techniques: Streaking, Plating, Pouring
• Instrumentation: Centrifuge, pH meter, Lyophilizer, Nanodrop, UV Spectrophotometer, Inverted microscope
• Biochemistry: Biochemical assays, Isolation and estimation of proteins
• Good laboratory practices

Academics

• [hidden] Biotechnology: Savitribai Phule Pune University – Sinhgad College of Engineering, CGPA [hidden]
• 12th Grade: [hidden] from Dr. Kalmaadi Shamrao Junior College, Pune
• 10th Grade: [hidden] from Abhinav Vidyalaya English Medium High School, Pune

Project

Fragrance production by biotransformation using mixed culture. The transformation of d-limonene was done using a mixed culture of Pseudomonas putida and rhodococcus erythropolis which resulted in fragrant intermediates. Immobilization technique was used to achieve maximum product formation. Cells were immobilized on Siran SIKUG41. Growing cell assays for product formation and limonene toxicity assay were performed to study the effects of limonene on cell growth. Quantification was done using Gas Chromatography. Field Emission Scanning Electron Microscopy (FESEM) was performed with free and immobilized cell beads.

Internship

Bioinformatics internship on Gene Expression Database for Human liver at Sinhagad College of Engineering, Pune.

Certifications

• Introduction to Genomic Technologies – [hidden]“>Certificate Link
• Marine Biology –