Raj Kishore – The Microbiologist Who is Battling Antimicrobial Resistance with Revolutionary Peptide Innovations!

Raj Kishore, PhD

Professional Summary

A dedicated and accomplished microbiologist with a PhD in Microbiology from CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, specializing in peptide biology, antimicrobial resistance, and ESKAPE pathogens. Proven expertise in high throughput in silico screening, peptide synthesis, microbiological assays, and preclinical evaluation of novel peptide mimetics. Skilled in animal handling, cell culture, molecular biology techniques, and advanced bioinformatics tools. Published multiple research articles and contributed to patented innovations in antimicrobial peptide development.

Areas of Expertise

• Microbiology: ESKAPE pathogens, antimicrobial resistance, biofilm studies, virology, mycology.
• Peptide Biology: Design, synthesis, and evaluation of antimicrobial and antiviral peptides.
• Molecular Biology: DNA/RNA isolation, PCR, RT-PCR, Western blotting, gel electrophoresis.
• Virology: RNA isolation and diagnostics of Covid-19 virus through RT-PCR.
• Mycology: Screening of compounds/nanoparticles against candida and aspergillus species.
• Hybridoma technology: Monoclonal antibody production.
• Animal Models: Thigh infection, lung infection, wound infection, and burn wound models.
• Bioinformatics: In silico screening, peptide design, Primer design.
• Instrumentation: HPLC, Lyophilizer, spectrophotometer, nanodrop, RT-PCR, BSL-2 laminar airflow.

Education

• PhD in Microbiology
  • CSIR-Central Drug Research Institute (CSIR-CDRI), Lucknow, India
  • Thesis: Exploring the Sensitivity of ESKAPE Pathogens Towards Novel Peptide Mimetics: An Answer to Antimicrobial Resistance
  • Supervisor: Dr. Mukesh Pasupuleti, Senior Principal Scientist, Microbiology Division
  • Year: 2021–2024 (Thesis Submitted)
• Master of Science ([hidden]) in Biotechnology
  • Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India
  • Score: [hidden]
  • Year: 2011–2013
• Bachelor of Science ([hidden]) in Chemistry and Microbiology
  • Deen Dayal Upadhyay Gorakhpur University, Uttar Pradesh, India
  • Score: [hidden]
  • Year: 2008–2011

Professional Experience

• ICMR-Senior Research Fellow (Peptide Biology and Antimicrobial resistance)
  • CSIR-Central Drug Research Institute, Lucknow, India
  • Duration: January 2021 – Present
  • Tasks:
    • Conducted high-throughput in silico screening of peptide analogs against ESKAPE pathogens.
    • Synthesized peptides using solid-phase peptide synthesis (SPPS) and evaluated their antimicrobial activity.
    • Developed and validated animal infection models (thigh, lung, and wound) for preclinical studies.
• Project Associate (Antiviral Research Diagnostics)
  • CSIR-Central Drug Research Institute, Lucknow, India
  • Duration: September 2020 – January 2021
  • Tasks: Conducted RNA isolation and detection of COVID-19 using RT-PCR.
• … (Please refer to the source for additional professional experiences)

Key Projects

• … (Please refer to the source for key projects details)

Skills

• Peptide Synthesis: Designing and Solid-phase peptide synthesis (SPPS)
• Animal Handling: Swiss, Balb/C, and C57BL/6 mice models
• Cell Culture: Maintenance of A549, VERO, THP-1, and BV2 cell lines
• … (Please refer to the source for additional skills)

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Cover Letter:

Dear Sir,
I am writing to express my keen interest in the Position of Sr data analyst . I have submitted my [hidden] in Microbiology under supervision of Professor Dr Mukesh Pasupuleti, CSIR–Central Drug Research Institute (CSIR-CDRI) Lucknow, India. My doctoral research focused on “Exploring the Sensitivity of ESKAPE Pathogens Towards Novel Peptide Mimetics: An Answer to Antimicrobial Resistance,” emphasizing the development of peptide-based therapeutics against drug-resistant pathogens. Throughout my [hidden] tenure, I have gained extensive experience in drug screening and optimization using various microbiological and cell culture assays.
In addition to my research experience, I possess expertise in a wide range of technical skills, including:

Microbiological and cell culture assays
ELISA, Western blotting, and monoclonal antibody development
Antifungal and antiviral screening
PCR, RT-PCR, and HPLC
Development of infectious disease models, such as lung infection, thigh infection, and burn wound models. along with my wet lab techniques in have data analysis utilizing statistical software such as GraphPad and SigmaPlot. Additionally, I am proficient in using tools like Photoshop, BioRender, MS Office, and AI-based software.
My multidisciplinary background in infectious disease modeling, immunology, and antimicrobial therapeutics enables me to contribute effectively to your team. I have a strong publication record in peer-reviewed journals and experience in patent drafting, reflecting my commitment to scientific innovation and translational research.

I would welcome the opportunity to discuss how my skills align with your research objectives. Please find my CV attached for your review. Thank you for your time and consideration. I look forward to the possibility of contributing to your team.

Best regards,
Raj Kishore
[hidden] Microbiology
CSIR–CDRI, Lucknow