Vaishnavi Pathradkar – Unlocking the Secrets of Biotechnology: A Rising Star’s Journey

Summary:

Vaishnavi Pathradkar

M.Sc. Biotechnology graduate with hands-on experience in laboratory research and techniques. Passionately looking forward to learning about emerging trends and techniques in the field. Highly adaptive to diverse working conditions. Meticulously documents observations in a concise and timely manner with a focus on accuracy and maintaining a sterile and organized work environment.

Education:

• MSc Biotechnology
  Symbiosis School of Biological Sciences, Symbiosis International (Deemed University), Pune
  2022-2024
• BSc Biotechnology
  Devi Ahilya Vishwavidyalaya, Indore
  2019-2022
• HSC
  Central Board of Secondary Education
  2018
• SSC
  Central Board of Secondary Education
  2016

Technical Skills:

• Microbiology: Pure culture isolation, streaking, media preparation, antimicrobial assay, antimicrobial sensitivity assay, CFU assay, Microscopy for microbial identification
• Molecular Biology: PCR, Electrophoresis, cDNA synthesis, DNA/RNA extraction, Plasmid isolation
• Virology: HA test, virus cultivation in tissue culture and embryonated chicken egg
• Animal Tissue Culture: Media preparation, Cell reviving and passaging, Live/dead cell count
• Immunology: Immunodiffusion assays, Blood grouping, ELISA
• Recombinant DNA Technology: Competent cells preparation, Bacterial transformation, Molecular cloning
• Biochemistry: Proteins, Carbohydrates, Amino acids estimation, Enzymology assays
• Bioinformatics: Sequence alignment (EMBOSS), BLAST
• Instruments: HPLC, GC, Spectrophotometer, lyophilizer, laminar flow cabinets, centrifuges, and incubators
• Data and statistical analysis, Microsoft Office, Teamwork, Strong Communication Skills, Decision-making and Problem Solving, Good Laboratory Practices (GLP), Standard Operating Procedures (SOP), Biosafety levels (BSL)

Research Experience:

• Investigation of Adaptive Responses of Lactiplantibacillus plantarum to Acidic pH Stress
  – Symbiosis School of Biological Sciences, Pune
  • Aim of the study was to develop an evolved strain of Lactiplantibacillus plantarum NCIM 2903 with enhanced acidic pH tolerance by performing Adaptive Laboratory Evolution (ALE) under acidic pH stress
  • Adapted populations were assessed to check acidic pH tolerance and cross tolerance to bile stress by CFU assay and fatty acid content by GC-FID
• PCR based incrimination of dengue virus in Aedes mosquito
  – ICMR-NIREH, Bhopal
  • Aim of the study was to detect the prevalence of dengue virus in Aedes mosquito
  • Further, RT-PCR, cDNA synthesis, and PCR amplification of cprm gene to detect the presence of dengue virus in Aedes mosquito
  • Lastly, serotyping of positive dengue samples via PCR amplification

Internship:

• Symbiosis School of Biological Sciences, Pune
  Performed various molecular techniques like nucleic acid isolation, PCR, cDNA synthesis, gel electrophoresis

Extracurricular Activities:

• Performed in AARAMBH 2022 and Y-20 summit 2023 – Symbiosis International University

Skills:

Microbiology, Molecular Biology, Virology, Animal Tissue Culture, Immunology, Recombinant DNA Technology, Biochemistry, Bioinformatics, Instruments, Data and Statistical Analysis, Microsoft Office, Teamwork, Strong Communication Skills, Decision Making and Problem Solving, Good Laboratory Practices (GLP), Standard Operating Procedures (SOP), Biosafety Levels (BSL)

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Cover Letter:

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