Shirin Bijapurkar – Discover the Future of Biotechnology with This Rising Star

Career Objective:
Passionate and inquisitive postgraduate in Biotechnology with a strong foundation in molecular biology, enzymology, and protein analysis. Seeking a role as a research scientist at the Ribosome Research Center to deepen understanding of ribosomal structure-function relationships, translation mechanisms, and molecular techniques in RNA and protein synthesis. Eager to contribute to ongoing research while building technical proficiency in ribosome-related methodologies.

1. Isolation and Characterization of Alkaline Lipase and Alkaline Protease Producing Microorganisms from Natural Sources:
Postgraduate project focuses on isolation, characterization, and production of alkaline lipase and alkaline protease-producing microorganisms. Enzyme-producing microorganisms were successfully isolated, identified, and enzyme production was carried out.

2. Isolation and Purification of Papain Enzyme:
Extraction and purification of papain enzyme from unripe papaya and its biochemical analysis. Enzyme efficiency was performed. Enzyme was successfully isolated, purified, and enzyme activity was analyzed by Biuret and Ninhydrin method.

M.Sc. in Biotechnology – Krishna Institute of Medical Sciences | 2024

B.Sc. in Biotechnology – Vivekanand College, Kolhapur | 2022

• 1st Prize – Student-Teacher Presentation Competition
• 2nd Rank – Semester 3 of Postgraduation
• 3rd Rank – Semester 2 of Postgraduation

• Sustainable Agriculture in Botany
• Experimental Biotechnology – by NPTEL
• Instrumentation in Life Sciences

Gokul Milk Processing Unit, Kolhapur – Internship
Gained industrial exposure to large-scale dairy processing and quality control protocols and understood basic operations of automated processing and hygiene monitoring.

• Proficient in nucleic acid extraction (DNA/RNA) and quantification from microbial and plant sources.
• Skilled in PCR amplification, gel electrophoresis, and enzymatic assays (Biuret, Ninhydrin).
• Experience with SDS-PAGE for protein profiling and preliminary purification.
• Conducted isolation and characterization of microbial enzymes like alkaline protease and lipase.
• Worked on purification and activity analysis of papain enzyme from plant sources.
• Familiar with basic molecular cloning techniques and bacterial transformation.
• Competent in handling lab instruments such as spectrophotometers, centrifuges, thermal cyclers, and incubators.
• Exposure to sterile technique and chromatography setups.
• Skilled in recording experimental protocols and data using MS Office tools.
• Detail-oriented in maintaining lab notebooks and generating basic analytical interpretations.
• Strong academic background, quick learner, and effective communicator.
• Committed to laboratory safety, cleanliness, and collaboration.

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Cover Letter:

Shirin Bijapurkar
New shahupuri, Kolhapur 416001
Maharashtra

Hiring Manager
Oracity lifesciences

Subject: Application for Research Scientist Ribosome research center

Dear Sir/Mam,
I am writing to express my interest in opportunity at the Ribosome Research Center. As a recent postgraduate in Biotechnology with hands-on experience in molecular biology and protein biochemistry, I am deeply fascinated by ribosomal mechanisms and translational control in cellular systems.

During my MSc, I conducted independent projects involving enzyme purification, biochemical assays, and protein analysis. These experiences have not only sharpened my laboratory skills but also deepened my curiosity for structural and functional protein studies—especially those related to ribosomes and their central role in gene expression.

An internship at your center would be an exciting opportunity for me to contribute to advanced molecular research, while gaining exposure to ribosome-specific techniques and analytical approaches. I am enthusiastic, diligent, and eager to learn from the esteemed work being carried out in your lab.

Thank you for considering my application. I look forward to the opportunity to discuss how I can support your research team.

Sincerely,
Shirin Bijapurkar