AMMAN SHAFEEN – Unlocking the Secrets of Microbial Innovation: A Journey Through Medical Biotechnology

AMMAN SHAFEEN

Professional Summary

Medical Biotechnology postgraduate with industry experience in microbiology, molecular biology, and quality assurance. Proficient in microbial testing, biochemical testing, PCR, ELISA, SDS-PAGE, and, with exposure to R&D, experimental design, and quality control. Eager to contribute to innovation in a scientific environment.

Education

• Master of Science (M.Sc.) – Medical Biotechnology, Pravara Institute of Medical Sciences (DU) | CGPA: 7.15 | 2023
• Bachelor of Science (B.Sc.) – Medical Biotechnology, Pravara Institute of Medical Sciences (DU) | 67.8% Aggregate | 2021
• Nano-Diploma in Nanomedicine, Edapt Learning | 2020

Professional Experience

DBT- BITP Intern | Mr. Biologist LLP | June 2024 – Dec 2024

• Quality Control- Microbiologist
• Conduct environment monitoring on day-to-day basis.
• Conducted microbial experiments including- Antimicrobial assay, Bacterial and fungal extraction, identification, pure culture preparation, specialized media preparation. Ensured laboratory quality control and compiled experimental reports.

Apprentice – IPQA | Sun Pharmaceutical Industries Limited | Jan 2024 – May 2024

• Monitored production processes and packing of parenteral products.
• Conducted visual inspection of injectable and lyophilized formulations.
• Maintained documentation in accordance with GMP and regulatory standards.
• Participated in audits and ensured product quality compliance.

Research Projects

• Production and Extraction of Biofilm from Biosurfactant Producing Bacillus spp.
• Molecular Docking Analysis: Investigated molecular interactions between biosurfactants from Bacillus spp. and proteins involved in biofilm formation and stability.
• GC-MS Phytochemical Profiling: Studied antioxidative and antidiabetic properties of methanolic extracts from Pithecellobium dulce seeds and fruit coats.

Technical Skills

Molecular Biology Techniques:

• PCR (Polymerase Chain Reaction)
• ELISA (Enzyme-Linked Immunosorbent Assay)
• SDS-PAGE Analysis
• DNA extraction and purification
• Sanger sequencing and Next-Generation sequencing

Microbiology & Cell Culture:

• Microorganism Identification Test (Microscopy and Biochemical)
• Environmental monitoring and Bioburden test
• Growth promotion test
• Bacterial and Endophytic fungi extraction and culturing
• Media preparation (basic and specialised)
• Endotoxin detection

Instrumentation:

• GC and HPLC
• UV spectrophotometry
• Micro pipette and Multichannel pipette

Laboratory operations:

• Laboratory Quality Control
• Aseptic and sterile handling (autoclaving and fumigation)
• Data Analysis & Experimental Report Compilation

Bioinformatics & Data Analysis:

• Molecular Docking (AutoDock 4.0)
• Phylogenetic Analysis
• Homology Modelling (Modeller)

Software & Systems:

• MS Office Suite
• Windows OS

Publications

• Journal Article:
• Shafeen, A. et al. (2025). Phytoconstituents analysis of ripe Pithecellobium dulce seeds and coats: Insights from Gas Chromatography-Mass Spectrometry (GC-MS) and bioactivity assessment. Journal of Applied and Natural Science, 17(1), 87-95. Link to Article

Certifications

• Certified Microsoft Office Specialist
• Training on “Molecular Principles of Diagnostic Methods” – PIMS (DU)
• Workshop on “Expression and Purification of Recombinant Proteins” – Merck Innovation Laboratory
• Training on “Bioinformatics Tools in Drug Discovery” – Pravara Institute of Medical Sciences

Skills: Molecular Biology, Microbiology, Quality Control, PCR, ELISA, SDS-PAGE, Biochemical Testing, Laboratory Operations, Research, Data Analysis, Bioinformatics, GMP Compliance

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Cover Letter:

To
The Hiring Manager
Hitech Formulations
Baddi, Himachal Pradesh

Subject: Application for the position of QC microbiologist.

Respected Hiring Manager,
I am writing to express my interest in the position of QC Microbiologist (Sr. Officer / Executive) at Hitech Formulations. With a postgraduate degree in Medical Biotechnology and hands-on experience in quality control and microbiology, I am confident in my ability to contribute effectively to your esteemed organization.
During my tenure at MrBiologist LLP as QC-microbiologist personnel, I was responsible for environmental monitoring, microbial media preparation, Growth Promotion Tests, and microbial culture techniques, including bacterial and fungal isolation. Additionally, my apprenticeship at Sun Pharmaceuticals enabled me to closely adhere to GMP guidelines while conducting inspections and maintaining documentation for parenteral products.
I possess a solid understanding of laboratory instrumentation and calibration procedures (autoclave, pH meter, UV spectroscopy, centrifuge), along with a disciplined approach to inventory management, sterilisation practices, and GDP compliance. My training in both academic and industrial settings has equipped me with the analytical skills and attention to detail essential for maintaining product quality and ensuring audit readiness.
I am highly drawn towards Hitech Formulation’s work and their commitment to quality. I am eager to bring my technical background, laboratory discipline, and passion for microbiology to your team.
Thank you for considering my application. I look forward to the opportunity to further discuss how I can contribute to your team.

Yours Sincerely,
Amman Shafeen.