Avula Saidevarshini – Unleashing the Future of Biotechnology: Meet the Rising Star in Cell and Molecular Biology!

Avula Saidevarshini

Summary

Dedicated and passionate Cell and Molecular Biologist, and Microbiologist with hands-on research experience, including a six-month dissertation project and eight months of practical research work. Holds a master’s degree in Genomic Science with a strong academic track record. Skilled in cell culture, fungal biology, and recombinant protein production using Pichia pastoris. Known for a diligent and detail-oriented approach, with a commitment to advancing scientific knowledge. Actively expanding expertise in innovative research and cell handling, and seeking dynamic opportunities to further grow in cutting-edge biological sciences.

Education

• Central University of Kerala
• M.Sc. Genomic Science
• First Class 78.3%

• Sri Vaishnavi Degree College
• B.Sc. Biotechnology, Chemistry, Computer Applications
• First Class 93%

• Sri Chaitanya Junior College
• Botany Zoology Physics Chemistry
• First Class 96.7%

• G.S.M High School
• SSLC
• First Class-10 GPA

Experience

RESEARCH INTERN – MOLECULAR BIOLOGY

Aug 2024 – May 2025

Phyx44 Labs Pvt Ltd

• Molecular Cloning, Vector Construction, and Plasmid Validation.
• Yeast, Fungal, and Bacterial Transformation for Strain Development.
• CRISPR-based Genome Editing and Plasmid Curing for Host Optimization.
• Recombinant Protein Expression and Induction Optimization in Various Media.
• SDS-PAGE and Protein Expression Analysis for High-Yield Clone Selection.

Skills

WET LAB

• Yeast & Fungal transformation
• Plasmid Isolation & Purification
• Electroporation
• Induction using different medias
• SDS-PAGE

• Competent cell preparation
• E.coli transformation
• NEB assembly
• PCR
• Curing of plasmid strains

• qRT-PCR
• shRNA Cloning
• DNA/RNA Isolations & Quantification
• Gel Electrophoresis
• Cell Culture Basic

• Microscopic Knowledge

TECHNICAL SKILLS

• Snap gene
• Benchling
• Image j analysis
• Alpha fold
• PyMOL Doc

• CRISPR Designing
• Microsoft Office
• Bioinformatics tools & databases
• Python language- basic knowledge

Courses & Certifications

• Protein Docking and Visualization – RGCB 2022
• Pipeline to python workshop – 2023
• CRISPR -CAS9: Gene Editing and Bioinformatics Perspectives – Dr. Omics labs 2025

Projects

• CRISPR-Driven Genetic Modulation for High-Titre Protein Expression in Yeast – 2024
• Cloning and Expression of shRNA to Knock-down HOXB9 Gene – Dissertation 2023

Awards

• PRATHIBHA AWARD – Government of Andhra Pradesh – 2015

Communication Skills

English, Telugu, Hindi

Keywords: Wet Lab, Technical Skills, Courses & Certifications, Projects, Awards, Communication Skills

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Cover Letter:

COVER LETTER
Avula Saidevarshini,
Kadapa (dist.), Andhra Pradesh,
04-07-2025
Hiring Manager,
D-NOME Biosciences Pvt. Ltd.
Hyderabad, India.
Subject: Application for Scientist Position – Molecular Biology.
Respected Hiring Manager,
I am writing to express my sincere interest in the Scientist position in Molecular Biology at D-NOME Biosciences, as advertised. With a strong academic background in Genomic Science and practical research experience in molecular cloning, CRISPR-based genome editing, and recombinant protein production, I am enthusiastic about contributing to your organization’s cutting-edge work in life sciences and therapeutics.
During my academic journey, I maintained a steadfast commitment to continuous learning and excellence in the field of Interdisciplinary Sciences, culminating in achieving top grades throughout my master’s program in Genomic Science at the Central University of Kerala. My master’s dissertation focused on the cloning and expression of shRNA to knock down the HOXB9 gene, aiming to understand its role in mesenchymal transition and its therapeutic implications in disease models. My professional journey as a Research Intern at Phyx44 Labs Pvt. Ltd. provided me with valuable hands-on experience, allowing me to refine my practical skills and instilling in me the importance of strategic experiment design with effective time management. My role involves molecular cloning, CRISPR-based genome editing, yeast and bacterial transformation, and recombinant protein expression analysis. These responsibilities have equipped me with a strong foundation in both experimental techniques and analytical understanding.
My research interests and technical background strongly align with the objectives of your team, and I am eager to contribute meaningfully to your innovative projects. With close to 1.5 years of cumulative hands-on research experience—including nearly 10 months in an industry research setting and 6 months of academic laboratory work during my master’s dissertation. While I may not yet meet the full 2-year benchmark, I bring practical, project-driven experience, adaptability, and the mindset to work and learn at the level expected. I am confident that my persistence, scientific curiosity, and ability to take initiative will allow me to integrate quickly and make meaningful contributions to your team.
Thank you for considering my application. I have attached my CV for your review. I would be grateful for the opportunity to further discuss how my background and motivation align with your current needs.