Summary: Dr. Srikanth Manupati is a dedicated researcher with a Ph.D. in Biological Sciences (Immunology) and expertise in gene therapy. He has a strong academic background with a focus on microbiology, genetics, and molecular biology. Dr. Manupati’s doctoral research explored the therapeutic potential of 3,3-diindolylmethane in treating psoriasis, showcasing his innovative approach to medical research.
Personal Information
2-2-185/24/40, Mallikarjuna Nagar, Amberpet, Hyderabad, Telangana-500013
Academic Background
- 2012-2015: B.Sc. Microbiology, Bhavans Vivekananda College of Science, Humanities and Commerce, Osmania University, Hyderabad, India
- 2015-2017: M.Sc. Microbiology, Bhavans Vivekananda College of Science, Humanities and Commerce, Osmania University, Hyderabad, India
- 2018-2019: PGDGC Genetics, Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Hyderabad, India
- 2019-2024: PhD Biological Sciences (Immunology), Vellore Institute of Technology, Vellore, India
- 2024: Post-doc Gene Therapy, Narayana Nethralaya Foundation, Bengaluru, India
Positions and Research Experience
- 2019: Junior Research Fellow at Indian Institute of Millets Research, Department of Biochemistry, Hyderabad, India
- 2020-2023: Teaching Cum Research Assistant at Vellore Institute of Technology, Vellore, Tamil Nadu, India
- Sep 24 – Mar 25: Postdoctoral Fellow at GROW Laboratory, Narayana Nethralaya, Bangalore, India
Research Focus
Worked on determining the efficacy of AAV dual vector editing in CD34+ cells in vivo. To demonstrate the best AAV serotype for CD34+ cell transduction in vivo and test different correction modes for sickle cell disease. Furthermore, to select the finest combination to go for clinical trials.
Doctoral Dissertation
My doctoral research focused on exploring the therapeutic potential of 3,3-diindolylmethane (DIM), a small molecule, as a novel inhibitor of the IL-17A/IL-17RA interaction for treating psoriasis. Unlike current IL-17A inhibitors, DIM aimed to target IL-17A signaling effectively, showcasing its promise as a multifaceted therapeutic agent for psoriasis.
Competitive Exams Cleared
| Exam | Year |
|---|---|
| GATE (Life Sciences) | 2009 |
| ASRB NET (ICAR) | 2009 |
Awards
2022: Raman Research Award
Publications
NCBI Bibliography URL: Publications Link
Total Publications: 5
Citations: 14; h-index: 2; i10-index: 0;
Certifications
- Biosafety Level training: BSL2, VIT, and Narayana Nethralaya for AAV production
- Genetic Counseling: Certified as a Genetic Counselor in 2019 from Osmania University, Hyderabad, India
- Animal Handling and Management Training: ICMR, National institute of nutrition (NIN), Hyderabad, India
Research Skills
- In Vitro: Cell culture, T cell activation, differentiation, and expansion
- In Vivo: Injections, Infection, Immunization, Necropsy, Blood and urine collection
- Viral Culture: Adeno-associated virus (AAV) Culture
- Wet Lab Techniques: Flow cytometry, Western Blotting, qRT-PCR, Immunohistochemistry, Gene cloning, ELISA
- Software: FlowJo, ImageJ, GraphPad Prism, Excel, BioRender
Student Trainees
- Ann Miriam Jose – 2020
- Subhashini – 2021
References
-
Dr. M. Rasool., Ph.D.
Professor & Director of Purchase
School of Bioscience and Technology, Vellore Institute of Technology, Vellore-632014, TN, India
Contact: [Email Hidden], [Email Hidden]
Mobile: + [Phone Hidden] -
Dr. ANAND S. B.,
Associate Professor
Department of Epidemiology and Public Health, Central University of Tamil Nadu, Neelakudi, Thiruvarur-610005, TN, India
Contact: [Email Hidden]
Mobile: + [Phone Hidden] -
Dr. A. Venkateshwari, FTAS
Professor of Genetics
Institute of Genetics & Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad – 500 016, India
Contact: [Email Hidden]
Tel: (O) 23403681
Mobile: + [Phone Hidden]
Keywords
Microbiology, Genetics, Immunology, Molecular Biology, Gene Therapy, Cell Culture, Immunohistochemistry
“`
Cover Letter:
** Dr. Srikanth Manupati**
2-2-185/24/40, Mallikarjuna Nagar
Amberpet, Hyderabad – 500013, Telangana
Date: July 2, 2025
**Hiring Manager**
D-NOME Biosciences
Hyderabad, Telangana, India
Dear Hiring Manager,
I am writing to express my interest in the position of **Scientist – Molecular Biology (B)** at D-NOME Biosciences. With a strong academic foundation (Ph.D. in Biological Sciences – Immunology from VIT), extensive laboratory experience, and postdoctoral work in gene therapy, I am confident in my ability to make meaningful contributions to your team.
My recent postdoctoral work at Narayana Nethralaya Foundation focused on AAV-mediated gene editing in CD34+ cells to develop curative strategies for sickle cell disease. This involved designing viral vectors, optimizing transduction protocols, and testing efficacy in in vivo models. During my Ph.D., I led a project exploring small-molecule inhibitors targeting IL-17A/IL-17RA interactions for psoriasis therapy, employing molecular cloning, protein expression, immunological assays, and flow cytometry. This dual exposure to both immunology and gene therapy has provided me with a unique, multidisciplinary perspective.
Key technical competencies that align with your requirements include:
· **Molecular cloning**, gene expression studies, and protein purification workflows.
· Experience with **flow cytometry**, Western blotting, qRT-PCR, ELISA, and immunohistochemistry.
· Skilled in **AKTA-based chromatography** and downstream analysis.
· Certified in **Biosafety Level 2**, genetic counseling, and animal handling (ICMR – NIN).
With a publication record in reputed journals and experience mentoring junior researchers, I am both a capable bench scientist and a collaborative team member. I am highly motivated to contribute to D-NOME’s mission of advancing molecular biology research and product development.
I have attached my CV for your consideration. I would be delighted to discuss how my skills and experiences can align with D-NOME’s goals. Thank you for your time and consideration.
Sincerely,
**Dr. Srikanth Manupati**
Leave a Reply