Shrilaxmi Bhat

CAREER OBJECTIVE

A self-initiated Biotechnology Undergraduate with a record of achievement and outstanding research skills. Comprehensive knowledge of all standards and Regulations with experience in a fast-paced laboratory environment. Motivated to perform both individually and in a team, seeking opportunities for continuous learning and growth.

EDUCATION

Bachelor of Engineering | Biotechnology
Aug 2015 – July 2019
Bapuji Institute of Engineering and Technology, Affiliated to VTU, Davangere, Karnataka, India

WORK EXPERIENCE

Project Assistant
Mar 21 – July 23
Institute of Bioinformatics and Applied Biotechnology – Synthetic and Genome Engineering Laboratory, Biotech Park, Bengaluru, Karnataka, India

Project: “AraC-based Biosensor for the Detection of Isoprene in E. coli”

Activities:

• Vector and primer Design using Snapgene Software
• Performing PCR, Restriction Digestion, Ligation, Infusion Based cloning
• Performing Agarose Gel Electrophoresis, SDS-PAGE, ELISA
• Handling instruments like Microplate Reader, spectrophotometer, qPCR, Centrifuge, etc.

Research Intern
Nov 2020 – Mar 2021
Innule Biosciences Pvt Ltd, Bengaluru, Karnataka, India

Activities:

• Plasmid Transformation, Protein Purification, SDS-PAGE, etc.

Intern
July 2020 – Sep 2020
Maani Diagnostic Centre Pvt Ltd, Bhatkal, India

Activities:

• Routine Analysis of Blood samples by performing Biochemical Tests

PROJECTS AND RESEARCH

• “AraC-based Biosensor for the Detection of Isoprene in E. coli”
• “Expanding the promoter Library for developing genetic circuits in Synechocystis sp. PCC 6803”
• “Areca Husk Fibers as Agro-waste to Value Added Products in Textile Sector”

PUBLICATIONS

• AC& S Omega – Manuscript Accepted “AraC-based Biosensor for the Detection of Isoprene in E. coli”
• International Journal of Advanced Research “Areca Husk Fibers as Agro-waste to Value Added Products in Textile Sector”

TECHNICAL SKILLS AND KNOWLEDGE

• Molecular Biology
• Microbiology
• Cell Biology
• Protein Isolation and Purification
• Cell Culture Techniques
• Analytical Techniques
• Bioinformatics Tools
• Bioinformatics Databases
• SPL Drug Listing and FDA Labelling
• Food and Drug Label Review
• Microsoft Excel, Powerpoint, Word

CERTIFICATIONS

• LSSSDC Certification
• ICH GCP E6 Certification
• Cellular and Molecular Diagnostics
• Clinical Research and Regulatory Compliance

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Cover Letter:

Dear Hiring Manager,
I am writing to express my interest in the position of Mol Bio Scientist** ** at your Organization as advertised. Having built a strong background in biotechnology and molecular biology with experience in the molecular biology approach for target identification, coupled with knowledge in lead optimization and metabolite identification and characterization. I also have hands-on experience in cell-based assays, coupled with a strong commitment to laboratory safety and data integrity. I am confident in my ability to contribute meaningfully to your team’s scientific objectives, the opportunity to contribute to your team has really excited me.
During my studies and work experience, I have understood biochemical and molecular biological principles properly in relation to the metabolic engineering. I am conversant with molecular biology experiments like designing primers and vector by Snap gene software, performing PCR, Restriction Cloning and Transformation of Genetic Circuit into bacteria. Screening the clone by PCR or Restriction Digestion for best clonal selection, Gene Expression Analysis. Moreover, I also performed the Cell-based Assay for metabolite recognition, specificity, sensitivity.
My research experience has been, to a large degree, oriented around applying metabolic engineering for Industrial Applications. I have used my molecular biology, biochemistry approach in designing the experiment for best metabolite recognition. This expertise is what enables sorting out high-throughput screening through 96-well plate-based assays, ensuring precision and reproducibility. I also have hands-on experience in Molecular Biology experiments, Biochemistry Assay, Analytical Techniques. Cell sorting by flow cytometry and FACS. I have also handled the qPCR instrument for m-RNA Expression level in the cell and analysis of the result.
Apart from the adequate technical skillset, I have experience in hands-on liquid handling systems such as TECAN, which made my work in the laboratory easier and much faster. In turn, it helped me to further develop skills and pay more attention to detail, trouble-shoot, and optimize protocols for several applications.
I am definitely excited about the opportunity to bring my skills and experience to your organization. My biochemistry and molecular biology background, alongside practical training in metabolic engineering and Molecular Biology, will help me fit well with the team. I am also proficient in handling HPLC, GCMS, LCMS analytical instruments since I have used it for identifying the compound and its characterization.
I look forward to the opportunity to discuss how my expertise meets the goals of your organization. Thank you for considering my application. I look forward to the opportunity to make my contribution toward the cutting-edge research in at your organization.
I am excited about the opportunity to bring this experience to your organization and help uphold your reputation for safe, compliant, and high-quality products. I would welcome the chance to further discuss how my skills and experience align with your team’s goals.
Thank you for considering my application. I look forward to the opportunity to contribute to your continued success.
Sincerely,
Shrilaxmi Bhat