Prachi Chauhan – Unlocking Molecular Mysteries: The Rising Star in Biotechnology

Professional Summary

Detail-oriented molecular biologist with a Master’s in Biotechnology and over 2 years of experience in molecular and cell biology research. Strong background in antibody validation workflows including immunoassays, Western blotting, immunofluorescence, and ELISA. Hands-on experience in exosome isolation, cfDNA/miRNA analysis, and gene expression profiling using RT-PCR/qPCR techniques. Adept at experimental planning, meticulous data recording, and scientific communication. Eager to contribute to innovative product validation programs and research excellence.

Education

• M.Sc. Biotechnology | Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP | 2017 – 2019 | CGPA: 9.0/10
• B.Sc. (Chemistry, Botany, Zoology) | Dr. Harisingh Gour Vishwavidyalaya, Sagar, MP | 2014 – 2017 | CGPA: 4.5/8

Professional Experience

Research Assistant | Dept. of Neurology, Banaras Hindu University | Apr 2023 – May 2024

• Worked on hospital-based clinical studies, managed biological data, and collaborated with healthcare professionals.
• Contributed to research documentation, literature mining, and analysis of patient data.

Research Assistant | ICMR-NIREH, Bhopal | Apr 2021 – May 2022

• Involved in exosome isolation, cfDNA and circulating miRNA analysis for cancer biosensing.
• Conducted RT-PCR, qPCR, ELISA, and nucleic acid extraction for molecular diagnostics.
• Maintained precise records in laboratory notebooks and generated publication-quality data.
• Supported experimental design and application-based validation strategies.

Intern | Reliance Industries Limited (BCIL-BITP) | Feb 2020 – Aug 2020

• Project on metabolic engineering of Cyanobacteria for ethanol production.
• Gained exposure in vector design, transformation, and expression analysis.

Key Skills & Techniques

– Immunoassays: Western blotting, ELISA, Immunofluorescence

– Molecular biology: DNA/RNA isolation, RT-PCR, qPCR, site-directed mutagenesis

– Exosome and miRNA analysis, cfDNA profiling

– Protein-protein interaction analysis using immunoprecipitation

– Software: SnapGene, Geneious Prime, Microsoft Office Suite

– Bioinformatics tools: BLAST, FASTA, sequence alignment

– Data interpretation, literature mining, and scientific reporting

Publications

• Surface-enhanced Raman scattering biosensors for detection of oncomiRs in breast cancer Drug Discovery Today (IF: 7.851)
• Integrated mitoepigenetic signalling mechanisms associated with airborne particulate matter exposure: A cross-sectional pilot study Atmospheric Pollution Research (IF: 4.352)
• Nano-engineered vitamins as a potential epigenetic modifier against environmental air pollutants Reviews on Environmental Health (IF: 2.95)
• Prenatal exposure to environmental pro-oxidants induces mitochondria-mediated epigenetic changes: a cross-sectional pilot study Environmental science & pollution research International (IF: 4.306)
• Graphene quantum-dot-based nanophotonic approach for targeted detection of long noncoding RNAs in circulation. ACS Omega (IF: 4.132)
• Mechanism of antibacterial activity of diallyl sulfide against Bacillus cereus. Journal of ayurveda and integrative medicine (IF: 1.7)

Achievements

• Best Poster Award at ISSRF International Conference on Reproductive Healthcare (2022)
• Organized clinical research workshop for doctors and nurses at BHU (2023)
• Certified in Pharmaceutical Production and Drug Discovery & Development by LSSSDC (2022)

Declaration

I hereby declare that the information provided above is true to the best of my knowledge. I am available to join immediately and am open to relocation.

Keywords: Immunoassays, Western blotting, ELISA, Immunofluorescence, DNA/RNA isolation, RT-PCR, qPCR, site-directed mutagenesis, Exosome, miRNA analysis, cfDNA profiling, Protein-protein interaction analysis, SnapGene, Geneious Prime, Microsoft Office Suite, BLAST, FASTA, sequence alignment, Data interpretation, Literature mining, Scientific reporting.

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Cover Letter:

**Prachi Chauhan**
Bhopal, Madhya Pradesh
Date: 03.07.2025

**Hiring Manager**
D-Nome Biosciences
Hyderabad, India

Dear Hiring Manager,
I am writing to express my strong interest in the Scientist – Molecular Biology position at D-Nome Biosciences, Hyderabad. With over two years of hands-on experience in molecular biology and protein biology techniques, I am confident in my ability to contribute effectively to your ongoing and future research programs.
During my previous roles, I have worked extensively with molecular cloning, PCR, plasmid construction, and protein expression and purification. My background includes experience in handling bacterial and mammalian systems, with a focus on optimizing experimental protocols and troubleshooting technical challenges efficiently. I am also well-versed in working within collaborative, interdisciplinary research teams, ensuring high-quality and reproducible results.
What particularly excites me about this opportunity is D-Nome Biosciences’ commitment to innovation in biotechnology and translational research. I am deeply motivated to be a part of such a dynamic environment where cutting-edge molecular biology research is being applied to real-world biological questions.
I am available to join immediately and would welcome the opportunity to contribute to your team. I am confident that my technical skills, research experience, and proactive attitude make me a strong candidate for this role.
Thank you for considering my application. I look forward to the opportunity to discuss how my background aligns with the goals of your organization.
Warm regards,
**Prachi Chauhan**