Dhruva Kumar Kesireddy – Unveiling the Next Breakthrough in Biotechnology and Molecular Research

DHRUVA KUMAR KESIREDDY

Hyderabad, Telangana

SUMMARY

Detail-oriented and skilled researcher with expertise in molecular biology, tissue processing, cell culture, immunohistochemistry, genomics, and chromatin accessibility assays. Strong background in lab operations, inventory management, and vendor coordination to ensure seamless workflow. Committed to driving scientific advancements through precision, efficiency, and innovative problem-solving in biotechnology and life sciences.

EDUCATION

2017 – 2020
Bachelors in Biotechnology, Microbiology and Chemistry
Keshav Memorial Institute of Commerce and Sciences – (affiliated to Osmania University), Hyderabad

2020-2022
Master’s in Animal Biology and Biotechnology
University of Hyderabad

WORK EXPERIENCE

2024 – Present
Project Associate I – CSIR – Centre for Cellular and Molecular Biology
Project Based Student Trainee, CSIR – Centre for Cellular and Molecular Biology

TECHNICAL SKILLS

• Molecular techniques: DNA isolation, Cut-and-Paste cloning, PCR-based cloning, Restriction digestion, Ligation, Transformation, Plasmid isolation (large and small quantities), Gel electrophoresis
• RNA isolation, cDNA preparation, qPCR
• Protein Techniques: Bicinchoninic Acid (BCA) assay, SDS-PAGE, Western blotting
• Tissue Processing: Embedding, slicing spinal cord and brain tissues, Immunohistochemistry, Microscopy techniques for histological analysis
• Cell Culture: Media preparation, Cell splitting, Immunofluorescence techniques
• Genomics: Nuclei Isolation, BULK-ATAC, CUT&RUN, Hi-C
• Software Skills: MS Word, MS Excel, MS PowerPoint, R programming for Data analysis and Visualization

LAB OPERATIONS SUPPORT

• Adeno Associated Virus (AAV) Production: Maintenance of HEK293T cells, Transfection, AAV Production, Cell Harvesting, AAV Purification, Titering, quality control

CERTIFICATIONS

• CMMDR2024 and IAN 2024 poster presentation

INTERNSHIP

Drug Discovery and Development, under the guidance of Dr. Mirza S Baig, IIT Indore

ACTIVITIES

• Organized and managed a stall at CCMB Open Day twice, educating children on Neurobiology

ACHIEVEMENTS

• Awarded Best Guide Prize at the Open Day events conducted by CSIR-CCMB (2023 & 2024)
• Received four gold medals during graduation
• Qualified national entrances like GAT-B and the University of Hyderabad entrance, TS-SET
• 2nd prize in PowerPoint presentation on International Science Day (KMICS)
• 1st prize in poster presentation competition (KMICS – Hematology symposium)
• 1st prize in JAM event on National Science Day (KMICS)
• 3rd prize in inter-college PPT competition on sustainability through horticulture (Andhra Mahila Sabha College)

LANGUAGES

English, Telugu, Hindi

ADDITIONAL INFORMATION

Skills: molecular biology, tissue processing, cell culture, immunohistochemistry, genomics, chromatin accessibility assays, lab operations, inventory management, vendor coordination, DNA isolation, PCR, Western blotting

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Cover Letter:

I am writing to express my keen interest in the position of Scientist – Molecular Biology at D-NOME Biosciences, as advertised. With a Master’s degree in Animal Biology and Biotechnology and over two years of extensive hands-on experience at CSIR – Centre for Cellular and Molecular Biology (CCMB), I am confident in my ability to contribute effectively to your interdisciplinary R&D team. At CCMB, my work has focused on a range of molecular biology techniques that align closely with your requirements: from restriction digestion, ligation, bacterial transformation, plasmid isolation, and PCR to more specialized methods such as bulk ATAC-seq, CUT&RUN, and Hi-C for chromatin accessibility and genomics studies. My familiarity with protein techniques including BCA assays, SDS-PAGE, and Western blotting further complements your core focus on recombinant protein expression and purification. Additionally, my experience includes designing and troubleshooting plasmid-based constructs, optimizing expression conditions and monitoring time-course studies, maintaining and transfecting HEK293T cells as part of AAV production workflows, proficiency with data analysis tools (including R) and strong documentation practices. Beyond technical skills, I have actively supported lab operations by coordinating vendor interactions, managing inventory, and training newcomers—demonstrating organizational skills and a commitment to team success. My enthusiasm for continuous learning and passion for translational research would make me a strong fit for D-NOME Biosciences’ mission. I would welcome the opportunity to discuss how my background can contribute to your ongoing projects in recombinant protein workflows and gene editing. Thank you for considering my application. I look forward to your response. Warm regards, Dhruva Kumar Kesireddy